Double gene targeting multiplex polymerase chain reaction–restriction fragment length polymorphism assay discriminates beef, buffalo, and pork substitution in frankfurter products
Beef, buffalo, and pork adulteration in the food chain is an emerging and sensitive issue. Current molecular techniques to authenticate these species depend on polymerase chain reaction (PCR) assays involving long and single targets which break down under natural decomposition and/or processing trea...
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American Chemical Society
2016
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| Online Access: | http://irep.iium.edu.my/53874/ http://irep.iium.edu.my/53874/ http://irep.iium.edu.my/53874/ http://irep.iium.edu.my/53874/2/53874_double%20gene%20targeting_Scopus.pdf http://irep.iium.edu.my/53874/8/53874_Double%20Gene%20Targeting%20Multiplex%20Polymerase%20Chain%20Reaction_wos.pdf http://irep.iium.edu.my/53874/9/53874_Double%20Gene%20Targeting%20Multiplex%20Polymerase%20Chain%20Reaction.pdf |
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iium-538742018-07-10T00:43:53Z http://irep.iium.edu.my/53874/ Double gene targeting multiplex polymerase chain reaction–restriction fragment length polymorphism assay discriminates beef, buffalo, and pork substitution in frankfurter products Hossain, Motalib A.Motalib Ali, Md. Eaqub Abd Hamid, Sharifah Bee Asing, Asing Mustafa, Shuhaimi Mohd Desa, Mohd Nasir Sarker, Md. Zaidul Islam R Medicine (General) Beef, buffalo, and pork adulteration in the food chain is an emerging and sensitive issue. Current molecular techniques to authenticate these species depend on polymerase chain reaction (PCR) assays involving long and single targets which break down under natural decomposition and/or processing treatments. This novel multiplex polymerase chain reaction–restriction fragment length polymorphism assay targeted two different gene sites for each of the bovine, buffalo, and porcine materials. This authentication ensured better security, first through a complementation approach because it is highly unlikely that both sites will be missing under compromised states, and second through molecular fingerprints. Mitochondrial cytochrome b and ND5 genes were targeted, and all targets (73, 90, 106, 120, 138, and 146 bp) were stable under extreme boiling and autoclaving treatments. Target specificity and authenticity were ensured through cross-amplification reaction and restriction digestion of PCR products with AluI, EciI, FatI, and CviKI-1 enzymes. A survey of Malaysian frankfurter products revealed rampant substitution of beef with buffalo but purity in porcine materials. American Chemical Society 2016-08-17 Article PeerReviewed application/pdf en http://irep.iium.edu.my/53874/2/53874_double%20gene%20targeting_Scopus.pdf application/pdf en http://irep.iium.edu.my/53874/8/53874_Double%20Gene%20Targeting%20Multiplex%20Polymerase%20Chain%20Reaction_wos.pdf application/pdf en http://irep.iium.edu.my/53874/9/53874_Double%20Gene%20Targeting%20Multiplex%20Polymerase%20Chain%20Reaction.pdf Hossain, Motalib A.Motalib and Ali, Md. Eaqub and Abd Hamid, Sharifah Bee and Asing, Asing and Mustafa, Shuhaimi and Mohd Desa, Mohd Nasir and Sarker, Md. Zaidul Islam (2016) Double gene targeting multiplex polymerase chain reaction–restriction fragment length polymorphism assay discriminates beef, buffalo, and pork substitution in frankfurter products. Journal of Agricultural and Food Chemistry, 64 (32). pp. 6343-6354. ISSN 0021-8561 E-ISSN 1520-5118 http://pubs.acs.org/doi/abs/10.1021/acs.jafc.6b02224 10.1021/acs.jafc.6b02224 |
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Digital Repository |
| institution_category |
Local University |
| institution |
International Islamic University Malaysia |
| building |
IIUM Repository |
| collection |
Online Access |
| language |
English English English |
| topic |
R Medicine (General) |
| spellingShingle |
R Medicine (General) Hossain, Motalib A.Motalib Ali, Md. Eaqub Abd Hamid, Sharifah Bee Asing, Asing Mustafa, Shuhaimi Mohd Desa, Mohd Nasir Sarker, Md. Zaidul Islam Double gene targeting multiplex polymerase chain reaction–restriction fragment length polymorphism assay discriminates beef, buffalo, and pork substitution in frankfurter products |
| description |
Beef, buffalo, and pork adulteration in the food chain is an emerging and sensitive issue. Current molecular techniques to authenticate these species depend on polymerase chain reaction (PCR) assays involving long and single targets which break down under natural decomposition and/or processing treatments. This novel multiplex polymerase chain reaction–restriction fragment length polymorphism assay targeted two different gene sites for each of the bovine, buffalo, and porcine materials. This authentication ensured better security, first through a complementation approach because it is highly unlikely that both sites will be missing under compromised states, and second through molecular fingerprints. Mitochondrial cytochrome b and ND5 genes were targeted, and all targets (73, 90, 106, 120, 138, and 146 bp) were stable under extreme boiling and autoclaving treatments. Target specificity and authenticity were ensured through cross-amplification reaction and restriction digestion of PCR products with AluI, EciI, FatI, and CviKI-1 enzymes. A survey of Malaysian frankfurter products revealed rampant substitution of beef with buffalo but purity in porcine materials. |
| format |
Article |
| author |
Hossain, Motalib A.Motalib Ali, Md. Eaqub Abd Hamid, Sharifah Bee Asing, Asing Mustafa, Shuhaimi Mohd Desa, Mohd Nasir Sarker, Md. Zaidul Islam |
| author_facet |
Hossain, Motalib A.Motalib Ali, Md. Eaqub Abd Hamid, Sharifah Bee Asing, Asing Mustafa, Shuhaimi Mohd Desa, Mohd Nasir Sarker, Md. Zaidul Islam |
| author_sort |
Hossain, Motalib A.Motalib |
| title |
Double gene targeting multiplex polymerase chain reaction–restriction fragment length polymorphism assay discriminates beef, buffalo, and pork substitution in frankfurter products |
| title_short |
Double gene targeting multiplex polymerase chain reaction–restriction fragment length polymorphism assay discriminates beef, buffalo, and pork substitution in frankfurter products |
| title_full |
Double gene targeting multiplex polymerase chain reaction–restriction fragment length polymorphism assay discriminates beef, buffalo, and pork substitution in frankfurter products |
| title_fullStr |
Double gene targeting multiplex polymerase chain reaction–restriction fragment length polymorphism assay discriminates beef, buffalo, and pork substitution in frankfurter products |
| title_full_unstemmed |
Double gene targeting multiplex polymerase chain reaction–restriction fragment length polymorphism assay discriminates beef, buffalo, and pork substitution in frankfurter products |
| title_sort |
double gene targeting multiplex polymerase chain reaction–restriction fragment length polymorphism assay discriminates beef, buffalo, and pork substitution in frankfurter products |
| publisher |
American Chemical Society |
| publishDate |
2016 |
| url |
http://irep.iium.edu.my/53874/ http://irep.iium.edu.my/53874/ http://irep.iium.edu.my/53874/ http://irep.iium.edu.my/53874/2/53874_double%20gene%20targeting_Scopus.pdf http://irep.iium.edu.my/53874/8/53874_Double%20Gene%20Targeting%20Multiplex%20Polymerase%20Chain%20Reaction_wos.pdf http://irep.iium.edu.my/53874/9/53874_Double%20Gene%20Targeting%20Multiplex%20Polymerase%20Chain%20Reaction.pdf |
| first_indexed |
2023-09-18T21:16:12Z |
| last_indexed |
2023-09-18T21:16:12Z |
| _version_ |
1777411578977583104 |