The effects of KGM, mannose and co-supplementation of KGM and mannose on mammalian cells cultured at inside and outside incubator conditions

Mammalian cells including cancer cells, stem cells and cell lines are important in the application of cells for therapies and research activities. They require the cells to be transported from the laboratory or surgery theater to the bedside, making it a challenge to be exposed at outside, shear str...

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Main Authors:	Marzuke, Ainul Bashirah, Wan Kamarul Zaman, Wan Safwani, Shahbuddin, Munira, Aung, Shuh Wen
Format:	Conference or Workshop Item
Language:	English English English
Published:	Springer Verlag 2016
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/53778/ http://irep.iium.edu.my/53778/ http://irep.iium.edu.my/53778/ http://irep.iium.edu.my/53778/16/53778_The%20effects%20of%20KGM%2C%20mannose%20and%20co-supplementation_lengkap.pdf http://irep.iium.edu.my/53778/17/53778_The%20effects%20of%20KGM%2C%20mannose_SCOPUS.pdf http://irep.iium.edu.my/53778/18/53778_The%20effects%20of%20KGM%2C%20mannose%20and%20co-supplementation_wos.pdf

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spelling	iium-537782019-08-25T07:13:54Z http://irep.iium.edu.my/53778/ The effects of KGM, mannose and co-supplementation of KGM and mannose on mammalian cells cultured at inside and outside incubator conditions Marzuke, Ainul Bashirah Wan Kamarul Zaman, Wan Safwani Shahbuddin, Munira Aung, Shuh Wen TP248.13 Biotechnology Mammalian cells including cancer cells, stem cells and cell lines are important in the application of cells for therapies and research activities. They require the cells to be transported from the laboratory or surgery theater to the bedside, making it a challenge to be exposed at outside, shear stress condition and differences especially in the tropical climate countries. In this research, Konjac Glucomannan (KGM) and D-mannose were examined for their potential use in the delivery of cells when subjected to differences in and outside incubator conditions. The objective of this work was to examine the risk and effects of different type of sugar supplementation in the transportation of cells. In this study, we used mammalian cells; stem cells from human extracted deciduous tooth (SHED), human keratinocyte cell lines (HaCaT) and human breast cancer (MCF-7). We hypothesized that the supplementations of KGM and D-mannose in the culture medium will act as protective agents to the cells due to their unique biological properties interacting via carbohydrate-protein interactions. Experiments were conducted in laboratory conditions to compare the effect of these sugars on the viability of different type of cells when placed at inside and outside incubator condition for 24, 48 and 72 hours. Evaluation of cellular viability and proliferation showed that co-supplementation of KGM and D-mannose inhibited the viability of MCF-7 at both inside and outside incubator conditions while supplementation of these sugars to other cells cultured at outside incubator condition did improve cellular viability and morphology compared to the cells cultured at inside the incubator, elucidating the potential benefit of sugar glycobiology in cellular transportation. © International Federation for Medical and Biological Engineering 2016. Springer Verlag 2016 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/53778/16/53778_The%20effects%20of%20KGM%2C%20mannose%20and%20co-supplementation_lengkap.pdf application/pdf en http://irep.iium.edu.my/53778/17/53778_The%20effects%20of%20KGM%2C%20mannose_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/53778/18/53778_The%20effects%20of%20KGM%2C%20mannose%20and%20co-supplementation_wos.pdf Marzuke, Ainul Bashirah and Wan Kamarul Zaman, Wan Safwani and Shahbuddin, Munira and Aung, Shuh Wen (2016) The effects of KGM, mannose and co-supplementation of KGM and mannose on mammalian cells cultured at inside and outside incubator conditions. In: International Conference for Innovation in Biomedical Engineering and Life Sciences, ICIBEL 2015, 6th-8th Dec 2015, Putrajaya. https://link.springer.com/chapter/10.1007/978-981-10-0266-3_43 10.1007/978-981-10-0266-3_43
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Marzuke, Ainul Bashirah Wan Kamarul Zaman, Wan Safwani Shahbuddin, Munira Aung, Shuh Wen The effects of KGM, mannose and co-supplementation of KGM and mannose on mammalian cells cultured at inside and outside incubator conditions
description	Mammalian cells including cancer cells, stem cells and cell lines are important in the application of cells for therapies and research activities. They require the cells to be transported from the laboratory or surgery theater to the bedside, making it a challenge to be exposed at outside, shear stress condition and differences especially in the tropical climate countries. In this research, Konjac Glucomannan (KGM) and D-mannose were examined for their potential use in the delivery of cells when subjected to differences in and outside incubator conditions. The objective of this work was to examine the risk and effects of different type of sugar supplementation in the transportation of cells. In this study, we used mammalian cells; stem cells from human extracted deciduous tooth (SHED), human keratinocyte cell lines (HaCaT) and human breast cancer (MCF-7). We hypothesized that the supplementations of KGM and D-mannose in the culture medium will act as protective agents to the cells due to their unique biological properties interacting via carbohydrate-protein interactions. Experiments were conducted in laboratory conditions to compare the effect of these sugars on the viability of different type of cells when placed at inside and outside incubator condition for 24, 48 and 72 hours. Evaluation of cellular viability and proliferation showed that co-supplementation of KGM and D-mannose inhibited the viability of MCF-7 at both inside and outside incubator conditions while supplementation of these sugars to other cells cultured at outside incubator condition did improve cellular viability and morphology compared to the cells cultured at inside the incubator, elucidating the potential benefit of sugar glycobiology in cellular transportation. © International Federation for Medical and Biological Engineering 2016.
format	Conference or Workshop Item
author	Marzuke, Ainul Bashirah Wan Kamarul Zaman, Wan Safwani Shahbuddin, Munira Aung, Shuh Wen
author_facet	Marzuke, Ainul Bashirah Wan Kamarul Zaman, Wan Safwani Shahbuddin, Munira Aung, Shuh Wen
author_sort	Marzuke, Ainul Bashirah
title	The effects of KGM, mannose and co-supplementation of KGM and mannose on mammalian cells cultured at inside and outside incubator conditions
title_short	The effects of KGM, mannose and co-supplementation of KGM and mannose on mammalian cells cultured at inside and outside incubator conditions
title_full	The effects of KGM, mannose and co-supplementation of KGM and mannose on mammalian cells cultured at inside and outside incubator conditions
title_fullStr	The effects of KGM, mannose and co-supplementation of KGM and mannose on mammalian cells cultured at inside and outside incubator conditions
title_full_unstemmed	The effects of KGM, mannose and co-supplementation of KGM and mannose on mammalian cells cultured at inside and outside incubator conditions
title_sort	effects of kgm, mannose and co-supplementation of kgm and mannose on mammalian cells cultured at inside and outside incubator conditions
publisher	Springer Verlag
publishDate	2016
url	http://irep.iium.edu.my/53778/ http://irep.iium.edu.my/53778/ http://irep.iium.edu.my/53778/ http://irep.iium.edu.my/53778/16/53778_The%20effects%20of%20KGM%2C%20mannose%20and%20co-supplementation_lengkap.pdf http://irep.iium.edu.my/53778/17/53778_The%20effects%20of%20KGM%2C%20mannose_SCOPUS.pdf http://irep.iium.edu.my/53778/18/53778_The%20effects%20of%20KGM%2C%20mannose%20and%20co-supplementation_wos.pdf
first_indexed	2023-09-18T21:16:04Z
last_indexed	2023-09-18T21:16:04Z
_version_	1777411570560663552

The effects of KGM, mannose and co-supplementation of KGM and mannose on mammalian cells cultured at inside and outside incubator conditions

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