Poly-microbia biofilms are Candida albicans strain and morphology dependent

Introduction: Oral biofilms are formed by various microorganisms such as C. albicans, Actinomyces naeslundii and Streptococcus mutans. Biofilm forming ability is one of the virulent factors of C. albicans and this may be involved oral carcinogenesis. Our objective was to assess C. albicans, A. naesl...

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Main Authors:	Arzmi, Mohd Hafiz, Dashper, Stuart, Reynolds, Eric, McCullough, Michael
Format:	Conference or Workshop Item
Language:	English
Published:	2015
Subjects:	QR Microbiology RK Dentistry
Online Access:	http://irep.iium.edu.my/53768/ http://irep.iium.edu.my/53768/7/53768.pdf

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recordtype	eprints
spelling	iium-537682017-03-16T01:30:26Z http://irep.iium.edu.my/53768/ Poly-microbia biofilms are Candida albicans strain and morphology dependent Arzmi, Mohd Hafiz Dashper, Stuart Reynolds, Eric McCullough, Michael QR Microbiology RK Dentistry Introduction: Oral biofilms are formed by various microorganisms such as C. albicans, Actinomyces naeslundii and Streptococcus mutans. Biofilm forming ability is one of the virulent factors of C. albicans and this may be involved oral carcinogenesis. Our objective was to assess C. albicans, A. naeslundii and S. mutans interaction in the formation of poly-microbial biofilms. Methods: Four C. albicans ATCC strains (32354, MYA-2876, 90234, and 18804), two HIV isolates (genotype-A and genotype-B), two oral cancer isolates (OC1 and OC2), A. naeslundii (NCTC 10301) and S. mutans (Ingbritt) were used in this study. To study intra-kingdom biofilm formation, 24 h culture of C. albicans, A. naeslundii and S. mutans grown in Sabauraud’s dextrose agar, blood agar and Todd-Hewitt yeast extract agar, respectively, were suspended in artificial saliva medium (ASM) or RPMI-1640 to give 106 cells mL-1, 107 cells mL-1 and 108 cells mL-1, respectively in the well of 96-well plate and incubated for 72 h at 37°C. Subsequently, biofilm was stained with crystal violet or XTT to measure biomass and metabolic activity, respectively, and the absorbance at 620nm wavelength measured. To study inter- kingdom biofilms, the same protocol was repeated, except combinations of microorganisms were inoculated in a same well. Results: Growth of C. albicans was predominantly by yeast and hyphal form when grown in ASM and RPMI-1640, respectively. Biofilm biomass of C. albicans co- cultured with A. naeslundii and/or S. mutans was C. albicans strain and morphology dependent. More often high biofilm biomass (HBB) observed in ASM-grown biofilm (43.7%), and more often high metabolic activity (HMA) was observed in RPMI- grown biofilm (65.6%). Conclusion: This study demonstrates that inter-kingdom biofilm biomass and metabolic activity of C. albicans, A. naeslundii and S. mutans is dependent on the strain and morphological form of C. albicans. 2015 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/53768/7/53768.pdf Arzmi, Mohd Hafiz and Dashper, Stuart and Reynolds, Eric and McCullough, Michael (2015) Poly-microbia biofilms are Candida albicans strain and morphology dependent. In: 55th Annual Scientific Meeting of the IADR Australia & New Zealand Division, 23rd-26th August 2015, Dunedin, New Zealand. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QR Microbiology RK Dentistry
spellingShingle	QR Microbiology RK Dentistry Arzmi, Mohd Hafiz Dashper, Stuart Reynolds, Eric McCullough, Michael Poly-microbia biofilms are Candida albicans strain and morphology dependent
description	Introduction: Oral biofilms are formed by various microorganisms such as C. albicans, Actinomyces naeslundii and Streptococcus mutans. Biofilm forming ability is one of the virulent factors of C. albicans and this may be involved oral carcinogenesis. Our objective was to assess C. albicans, A. naeslundii and S. mutans interaction in the formation of poly-microbial biofilms. Methods: Four C. albicans ATCC strains (32354, MYA-2876, 90234, and 18804), two HIV isolates (genotype-A and genotype-B), two oral cancer isolates (OC1 and OC2), A. naeslundii (NCTC 10301) and S. mutans (Ingbritt) were used in this study. To study intra-kingdom biofilm formation, 24 h culture of C. albicans, A. naeslundii and S. mutans grown in Sabauraud’s dextrose agar, blood agar and Todd-Hewitt yeast extract agar, respectively, were suspended in artificial saliva medium (ASM) or RPMI-1640 to give 106 cells mL-1, 107 cells mL-1 and 108 cells mL-1, respectively in the well of 96-well plate and incubated for 72 h at 37°C. Subsequently, biofilm was stained with crystal violet or XTT to measure biomass and metabolic activity, respectively, and the absorbance at 620nm wavelength measured. To study inter- kingdom biofilms, the same protocol was repeated, except combinations of microorganisms were inoculated in a same well. Results: Growth of C. albicans was predominantly by yeast and hyphal form when grown in ASM and RPMI-1640, respectively. Biofilm biomass of C. albicans co- cultured with A. naeslundii and/or S. mutans was C. albicans strain and morphology dependent. More often high biofilm biomass (HBB) observed in ASM-grown biofilm (43.7%), and more often high metabolic activity (HMA) was observed in RPMI- grown biofilm (65.6%). Conclusion: This study demonstrates that inter-kingdom biofilm biomass and metabolic activity of C. albicans, A. naeslundii and S. mutans is dependent on the strain and morphological form of C. albicans.
format	Conference or Workshop Item
author	Arzmi, Mohd Hafiz Dashper, Stuart Reynolds, Eric McCullough, Michael
author_facet	Arzmi, Mohd Hafiz Dashper, Stuart Reynolds, Eric McCullough, Michael
author_sort	Arzmi, Mohd Hafiz
title	Poly-microbia biofilms are Candida albicans strain and morphology dependent
title_short	Poly-microbia biofilms are Candida albicans strain and morphology dependent
title_full	Poly-microbia biofilms are Candida albicans strain and morphology dependent
title_fullStr	Poly-microbia biofilms are Candida albicans strain and morphology dependent
title_full_unstemmed	Poly-microbia biofilms are Candida albicans strain and morphology dependent
title_sort	poly-microbia biofilms are candida albicans strain and morphology dependent
publishDate	2015
url	http://irep.iium.edu.my/53768/ http://irep.iium.edu.my/53768/7/53768.pdf
first_indexed	2023-09-18T21:16:03Z
last_indexed	2023-09-18T21:16:03Z
_version_	1777411569250992128

Poly-microbia biofilms are Candida albicans strain and morphology dependent

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