Co-aggregation: Interaction of Candida albicans, Actinomyces naeslundii and Streptococcus mutans

Introduction: Microbial interactions have been found to be associated with the development of oral biofilms. Inter-kingdom co-aggregation within biofilms may be important in enhancing adherence, altering epithelial cytokines, which may ultimately be involved in carcinogenesis. Objective: Our aim wa...

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Main Authors:	Arzmi, Mohd Hafiz, Dashper, Stuart, Reynolds, Eric, McCullough, Michael
Format:	Conference or Workshop Item
Language:	English
Published:	2014
Subjects:	QR Microbiology RK Dentistry
Online Access:	http://irep.iium.edu.my/53742/ http://irep.iium.edu.my/53742/ http://irep.iium.edu.my/53742/7/53742.pdf

id	iium-53742
recordtype	eprints
spelling	iium-537422017-03-16T00:36:32Z http://irep.iium.edu.my/53742/ Co-aggregation: Interaction of Candida albicans, Actinomyces naeslundii and Streptococcus mutans Arzmi, Mohd Hafiz Dashper, Stuart Reynolds, Eric McCullough, Michael QR Microbiology RK Dentistry Introduction: Microbial interactions have been found to be associated with the development of oral biofilms. Inter-kingdom co-aggregation within biofilms may be important in enhancing adherence, altering epithelial cytokines, which may ultimately be involved in carcinogenesis. Objective: Our aim was to determine co-aggregation of C. albicans (CA), A. naeslundii (AN), and S. mutans (SM). Methods: Four C. albicans ATCC strains (32354, MYA-2876, 90234, and 18804), two HIV isolates (genotype-A and genotype-B), 2 oral cancer isolates (OC1 and OC2), A. naeslundii (NCTC 10301) and S. mutans (Ingbritt) were used in this study. In-auto- aggregation assay, C. albicans was grown in RPMI-1640 and artificial saliva medium (ASM) to produce hyphae and yeast forms respectively, whereas bacteria were grown in BHI. C.albicans, A. naeslundii and S. mutans were standardised to give 106 cells/mL, 107 cells/mL and 108 cells/mL respectively, in co-aggregation buffer followed by a 1h incubation at 25°C. The absorbance difference (ΔAbs) between 0h and 1h was recorded. To study co-aggregation, the same protocol was repeated, except combinations of microorganisms were inoculated in the same vial. Results: The mean ΔAbs percentage of C. albicans was significantly higher in ASM than RPMI-1640. The mean ΔAbs percentage of CA-AN, CA-SM and CA-AN-SM was 25.6%, 23.2% and 42.1% lower than C. albicans alone, respectively, in RPMI-1640, whereas in ASM, only CA-AN had a lower ΔAbs percentage by 15.3%. CA-SM and CA-AN-SM in ASM increased ΔAbs percentage by 6.5% and 23.6%, respectively. Conclusion: This study demonstrates that inter-kingdom aggregation of C. albicans, A. naeslundii and S. mutans is dependent on the morphological form of C. albicans. Co-aggregation occurs much more in the yeast than the hyphal form, which may have a relevance to the development of oral biofilms and ultimately oral carcinogenesis. 2014 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/53742/7/53742.pdf Arzmi, Mohd Hafiz and Dashper, Stuart and Reynolds, Eric and McCullough, Michael (2014) Co-aggregation: Interaction of Candida albicans, Actinomyces naeslundii and Streptococcus mutans. In: 54th Annual Scientific Meeting of the International Association for Dental Research Australian & New Zealand Division, 28th September-1st October 2014, Brisbane, Australia. (Unpublished) http://www.iadranz.org.au/media/pdf/Program%20Booklet%20280914.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QR Microbiology RK Dentistry
spellingShingle	QR Microbiology RK Dentistry Arzmi, Mohd Hafiz Dashper, Stuart Reynolds, Eric McCullough, Michael Co-aggregation: Interaction of Candida albicans, Actinomyces naeslundii and Streptococcus mutans
description	Introduction: Microbial interactions have been found to be associated with the development of oral biofilms. Inter-kingdom co-aggregation within biofilms may be important in enhancing adherence, altering epithelial cytokines, which may ultimately be involved in carcinogenesis. Objective: Our aim was to determine co-aggregation of C. albicans (CA), A. naeslundii (AN), and S. mutans (SM). Methods: Four C. albicans ATCC strains (32354, MYA-2876, 90234, and 18804), two HIV isolates (genotype-A and genotype-B), 2 oral cancer isolates (OC1 and OC2), A. naeslundii (NCTC 10301) and S. mutans (Ingbritt) were used in this study. In-auto- aggregation assay, C. albicans was grown in RPMI-1640 and artificial saliva medium (ASM) to produce hyphae and yeast forms respectively, whereas bacteria were grown in BHI. C.albicans, A. naeslundii and S. mutans were standardised to give 106 cells/mL, 107 cells/mL and 108 cells/mL respectively, in co-aggregation buffer followed by a 1h incubation at 25°C. The absorbance difference (ΔAbs) between 0h and 1h was recorded. To study co-aggregation, the same protocol was repeated, except combinations of microorganisms were inoculated in the same vial. Results: The mean ΔAbs percentage of C. albicans was significantly higher in ASM than RPMI-1640. The mean ΔAbs percentage of CA-AN, CA-SM and CA-AN-SM was 25.6%, 23.2% and 42.1% lower than C. albicans alone, respectively, in RPMI-1640, whereas in ASM, only CA-AN had a lower ΔAbs percentage by 15.3%. CA-SM and CA-AN-SM in ASM increased ΔAbs percentage by 6.5% and 23.6%, respectively. Conclusion: This study demonstrates that inter-kingdom aggregation of C. albicans, A. naeslundii and S. mutans is dependent on the morphological form of C. albicans. Co-aggregation occurs much more in the yeast than the hyphal form, which may have a relevance to the development of oral biofilms and ultimately oral carcinogenesis.
format	Conference or Workshop Item
author	Arzmi, Mohd Hafiz Dashper, Stuart Reynolds, Eric McCullough, Michael
author_facet	Arzmi, Mohd Hafiz Dashper, Stuart Reynolds, Eric McCullough, Michael
author_sort	Arzmi, Mohd Hafiz
title	Co-aggregation: Interaction of Candida albicans, Actinomyces naeslundii and Streptococcus mutans
title_short	Co-aggregation: Interaction of Candida albicans, Actinomyces naeslundii and Streptococcus mutans
title_full	Co-aggregation: Interaction of Candida albicans, Actinomyces naeslundii and Streptococcus mutans
title_fullStr	Co-aggregation: Interaction of Candida albicans, Actinomyces naeslundii and Streptococcus mutans
title_full_unstemmed	Co-aggregation: Interaction of Candida albicans, Actinomyces naeslundii and Streptococcus mutans
title_sort	co-aggregation: interaction of candida albicans, actinomyces naeslundii and streptococcus mutans
publishDate	2014
url	http://irep.iium.edu.my/53742/ http://irep.iium.edu.my/53742/ http://irep.iium.edu.my/53742/7/53742.pdf
first_indexed	2023-09-18T21:16:00Z
last_indexed	2023-09-18T21:16:00Z
_version_	1777411566199635968

Co-aggregation: Interaction of Candida albicans, Actinomyces naeslundii and Streptococcus mutans

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