Method for preparing plasmid DNA
A METHOD FOR PREPARING PLASMID DNA, COMPRISING THE STEPS OF CULTURING HOST CELLS CONTAINING PLASMID DNA; LYSING THE HOST CELLS TO OBTAIN A LYSATE; ISOLATING SAID PLASMID DNA FROM SAID LYSATE TO FORM ISOLATED PLASMID DNA; AND PURIFYING THE ISOLATED PLASMID DNA; CHARACTERIZED IN THAT THE LYSATE IS SUB...
| Main Authors: | , , |
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| Format: | Patent |
| Language: | English |
| Published: |
2015
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/53741/ http://irep.iium.edu.my/53741/ http://irep.iium.edu.my/53741/ http://irep.iium.edu.my/53741/1/METHOD%20FOR%20PREPARING%20PLASMID%20DNA.pdf |
| Summary: | A METHOD FOR PREPARING PLASMID DNA, COMPRISING THE STEPS OF CULTURING HOST CELLS CONTAINING PLASMID DNA; LYSING THE HOST CELLS TO OBTAIN A LYSATE; ISOLATING SAID PLASMID DNA FROM SAID LYSATE TO FORM ISOLATED PLASMID DNA; AND PURIFYING THE ISOLATED PLASMID DNA; CHARACTERIZED IN THAT THE LYSATE IS SUBSTANTIALLY LYOPHILIZED AND SUBSEQUENTLY RESUSPENDED WITH WATER, PRIOR TO ISOLATION. (FIG 5A) |
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