Coaggregation of Candida albicans, Actinomyces naeslundii and Streptococcus mutans is Candida albicans strain dependent
Microbial interactions are necessarily associated with the development of polymicrobial oral bio lms. The objective of this study was to determine the coaggregation of eight strains of Candida albicans with Actinomyces naeslundii and Streptococcus mutans. In autoaggregation assays, C. albicans strai...
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Wiley-Blackwell
2015
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| Online Access: | http://irep.iium.edu.my/53622/ http://irep.iium.edu.my/53622/1/Co-aggregation%20of%20C.%20Albicans%2C%20A.%20naeslundii%20and%20S.%20mutans.pdf |
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iium-536222017-01-02T07:27:06Z http://irep.iium.edu.my/53622/ Coaggregation of Candida albicans, Actinomyces naeslundii and Streptococcus mutans is Candida albicans strain dependent Arzmi, Mohd Hafiz Stuart, Dashper Nicola, Cirillo Eric, Reynolds Michael, McCullough QR Microbiology RK Dentistry Microbial interactions are necessarily associated with the development of polymicrobial oral bio lms. The objective of this study was to determine the coaggregation of eight strains of Candida albicans with Actinomyces naeslundii and Streptococcus mutans. In autoaggregation assays, C. albicans strains were grown in RPMI-1640 and arti cial saliva medium (ASM) whereas bacteria were grown in heart infusion broth. C. albicans, A. naeslundii and S. mutans were suspended to give 106, 107 and 108 cells mL−1 respectively, in coaggregation buffer followed by a 1 h incubation. The absorbance difference at 620 nm ( Abs) between 0 h and 1 h was recorded. To study coaggregation, the same protocol was used, except combinations of microorganisms were incubated together. The mean Abs% of autoaggregation of the majority of RPMI-1640-grown C. albicans was higher than in ASM grown. Coaggregation of C. albicans with A. naeslundii and/or S. mutans was variable among C. albicans strains. Scanning electron microscopy images showed that A. naeslundii and S. mutans coaggregated with C. albicans in dual- and triculture. In conclusion, the coaggregation of C. albicans, A. naeslundii and S. mutans is C. albicans strain dependent. Wiley-Blackwell 2015-06-08 Article PeerReviewed application/pdf en http://irep.iium.edu.my/53622/1/Co-aggregation%20of%20C.%20Albicans%2C%20A.%20naeslundii%20and%20S.%20mutans.pdf Arzmi, Mohd Hafiz and Stuart, Dashper and Nicola, Cirillo and Eric, Reynolds and Michael, McCullough (2015) Coaggregation of Candida albicans, Actinomyces naeslundii and Streptococcus mutans is Candida albicans strain dependent. FEMS Yeast Research, 15 (5). pp. 1-7. ISSN 1567-1356 E-ISSN 1567-1364 |
| repository_type |
Digital Repository |
| institution_category |
Local University |
| institution |
International Islamic University Malaysia |
| building |
IIUM Repository |
| collection |
Online Access |
| language |
English |
| topic |
QR Microbiology RK Dentistry |
| spellingShingle |
QR Microbiology RK Dentistry Arzmi, Mohd Hafiz Stuart, Dashper Nicola, Cirillo Eric, Reynolds Michael, McCullough Coaggregation of Candida albicans, Actinomyces naeslundii and Streptococcus mutans is Candida albicans strain dependent |
| description |
Microbial interactions are necessarily associated with the development of polymicrobial oral bio lms. The objective of this study was to determine the coaggregation of eight strains of Candida albicans with Actinomyces naeslundii and Streptococcus mutans. In autoaggregation assays, C. albicans strains were grown in RPMI-1640 and arti cial saliva medium (ASM) whereas bacteria were grown in heart infusion broth. C. albicans, A. naeslundii and S. mutans were suspended to give 106, 107 and
108 cells mL−1 respectively, in coaggregation buffer followed by a 1 h incubation. The absorbance difference at 620 nm ( Abs) between 0 h and 1 h was recorded. To study coaggregation, the same protocol was used, except combinations of microorganisms were incubated together. The mean Abs% of autoaggregation of the majority of RPMI-1640-grown
C. albicans was higher than in ASM grown. Coaggregation of C. albicans with A. naeslundii and/or S. mutans was variable among C. albicans strains. Scanning electron microscopy images showed that A. naeslundii and S. mutans coaggregated with C. albicans in dual- and triculture. In conclusion, the coaggregation of C. albicans, A. naeslundii and S. mutans is C. albicans strain dependent. |
| format |
Article |
| author |
Arzmi, Mohd Hafiz Stuart, Dashper Nicola, Cirillo Eric, Reynolds Michael, McCullough |
| author_facet |
Arzmi, Mohd Hafiz Stuart, Dashper Nicola, Cirillo Eric, Reynolds Michael, McCullough |
| author_sort |
Arzmi, Mohd Hafiz |
| title |
Coaggregation of Candida albicans, Actinomyces
naeslundii and Streptococcus mutans is Candida albicans
strain dependent |
| title_short |
Coaggregation of Candida albicans, Actinomyces
naeslundii and Streptococcus mutans is Candida albicans
strain dependent |
| title_full |
Coaggregation of Candida albicans, Actinomyces
naeslundii and Streptococcus mutans is Candida albicans
strain dependent |
| title_fullStr |
Coaggregation of Candida albicans, Actinomyces
naeslundii and Streptococcus mutans is Candida albicans
strain dependent |
| title_full_unstemmed |
Coaggregation of Candida albicans, Actinomyces
naeslundii and Streptococcus mutans is Candida albicans
strain dependent |
| title_sort |
coaggregation of candida albicans, actinomyces
naeslundii and streptococcus mutans is candida albicans
strain dependent |
| publisher |
Wiley-Blackwell |
| publishDate |
2015 |
| url |
http://irep.iium.edu.my/53622/ http://irep.iium.edu.my/53622/1/Co-aggregation%20of%20C.%20Albicans%2C%20A.%20naeslundii%20and%20S.%20mutans.pdf |
| first_indexed |
2023-09-18T21:15:51Z |
| last_indexed |
2023-09-18T21:15:51Z |
| _version_ |
1777411556727848960 |