Effect of phenotypic switching on the biological properties and susceptibility to chlorhexidine in Candida krusei ATCC 14243

Phenotypic switching is characterized as a virulence factor of Candida spp. This study was carried out to evaluate the phenotypic switching ability of C. krusei ATCC 14243 and to determine its effect on the biological properties, adherence capacity and susceptibility towards chlorhexidine digluconat...

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Main Authors:	Arzmi, Mohd Hafiz, Abdul Razak, Fathilah, Musa, Md Yusoff, Wan Harun, Wan Himratul Aznita
Format:	Article
Language:	English
Published:	Wiley-Blackwell 2012
Subjects:	QR Microbiology RK Dentistry
Online Access:	http://irep.iium.edu.my/53621/ http://irep.iium.edu.my/53621/1/Effect%20of%20phenotypic%20switching%20on%20the%20biological%20properties%20and%20susceptibility%20to%20chlorhexidine%20in%20Candida%20krusei%20ATCC%2014243.pdf

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spelling	iium-536212016-12-30T03:57:56Z http://irep.iium.edu.my/53621/ Effect of phenotypic switching on the biological properties and susceptibility to chlorhexidine in Candida krusei ATCC 14243 Arzmi, Mohd Hafiz Abdul Razak, Fathilah Musa, Md Yusoff Wan Harun, Wan Himratul Aznita QR Microbiology RK Dentistry Phenotypic switching is characterized as a virulence factor of Candida spp. This study was carried out to evaluate the phenotypic switching ability of C. krusei ATCC 14243 and to determine its effect on the biological properties, adherence capacity and susceptibility towards chlorhexidine digluconate (CHX). To induce switched generations C. krusei was cultured under nitrogen-depleted growth conditions by adding phloxine B. These phenotypically switched colo- nies were designated as the 1st generation. Subsequent sub-culturing was per- formed to produce the 2nd, 3rd and 4th switched generations. The recovery of the 3rd generation was the highest at 85.7% while that of the 4th generation was lower at 70.8%, and the recovery of the 1st and 2nd generations gradually reduced to 46.6% and 36.4%, respectively. All generations of C. krusei were susceptible towards CHX. The unswitched C. krusei was the most susceptible but the least adherent to coated hard surfaces. The 2nd generation was the least susceptible, but with the highest adherent ability. The minimum inhibition concentration and minimal fungicidal concentration of C. krusei of all genera- tions were determined at 0.4 mg mL 1. These observations suggest that the switching activity of C. krusei induces changes to its biological properties and susceptibility towards CHX. Wiley-Blackwell 2012-05 Article PeerReviewed application/pdf en http://irep.iium.edu.my/53621/1/Effect%20of%20phenotypic%20switching%20on%20the%20biological%20properties%20and%20susceptibility%20to%20chlorhexidine%20in%20Candida%20krusei%20ATCC%2014243.pdf Arzmi, Mohd Hafiz and Abdul Razak, Fathilah and Musa, Md Yusoff and Wan Harun, Wan Himratul Aznita (2012) Effect of phenotypic switching on the biological properties and susceptibility to chlorhexidine in Candida krusei ATCC 14243. FEMS Yeast Research, 12 (3). pp. 351-358. ISSN 1567-1356 E-ISSN 1567-1364
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QR Microbiology RK Dentistry
spellingShingle	QR Microbiology RK Dentistry Arzmi, Mohd Hafiz Abdul Razak, Fathilah Musa, Md Yusoff Wan Harun, Wan Himratul Aznita Effect of phenotypic switching on the biological properties and susceptibility to chlorhexidine in Candida krusei ATCC 14243
description	Phenotypic switching is characterized as a virulence factor of Candida spp. This study was carried out to evaluate the phenotypic switching ability of C. krusei ATCC 14243 and to determine its effect on the biological properties, adherence capacity and susceptibility towards chlorhexidine digluconate (CHX). To induce switched generations C. krusei was cultured under nitrogen-depleted growth conditions by adding phloxine B. These phenotypically switched colo- nies were designated as the 1st generation. Subsequent sub-culturing was per- formed to produce the 2nd, 3rd and 4th switched generations. The recovery of the 3rd generation was the highest at 85.7% while that of the 4th generation was lower at 70.8%, and the recovery of the 1st and 2nd generations gradually reduced to 46.6% and 36.4%, respectively. All generations of C. krusei were susceptible towards CHX. The unswitched C. krusei was the most susceptible but the least adherent to coated hard surfaces. The 2nd generation was the least susceptible, but with the highest adherent ability. The minimum inhibition concentration and minimal fungicidal concentration of C. krusei of all genera- tions were determined at 0.4 mg mL 1. These observations suggest that the switching activity of C. krusei induces changes to its biological properties and susceptibility towards CHX.
format	Article
author	Arzmi, Mohd Hafiz Abdul Razak, Fathilah Musa, Md Yusoff Wan Harun, Wan Himratul Aznita
author_facet	Arzmi, Mohd Hafiz Abdul Razak, Fathilah Musa, Md Yusoff Wan Harun, Wan Himratul Aznita
author_sort	Arzmi, Mohd Hafiz
title	Effect of phenotypic switching on the biological properties and susceptibility to chlorhexidine in Candida krusei ATCC 14243
title_short	Effect of phenotypic switching on the biological properties and susceptibility to chlorhexidine in Candida krusei ATCC 14243
title_full	Effect of phenotypic switching on the biological properties and susceptibility to chlorhexidine in Candida krusei ATCC 14243
title_fullStr	Effect of phenotypic switching on the biological properties and susceptibility to chlorhexidine in Candida krusei ATCC 14243
title_full_unstemmed	Effect of phenotypic switching on the biological properties and susceptibility to chlorhexidine in Candida krusei ATCC 14243
title_sort	effect of phenotypic switching on the biological properties and susceptibility to chlorhexidine in candida krusei atcc 14243
publisher	Wiley-Blackwell
publishDate	2012
url	http://irep.iium.edu.my/53621/ http://irep.iium.edu.my/53621/1/Effect%20of%20phenotypic%20switching%20on%20the%20biological%20properties%20and%20susceptibility%20to%20chlorhexidine%20in%20Candida%20krusei%20ATCC%2014243.pdf
first_indexed	2023-09-18T21:15:51Z
last_indexed	2023-09-18T21:15:51Z
_version_	1777411556583145472

Effect of phenotypic switching on the biological properties and susceptibility to chlorhexidine in Candida krusei ATCC 14243

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