Biotechnological potential of Kuantan mangrove actinomycete, Micromonospora K3-13

Introduction Mangrove is a complex and unique ecosystem which experiences periodical tidal flooding inundated with low, moderate and high saline water. The muddy soil is rich in organic matter derived from efficient nutrient cycling system and decomposition of mangrove leaves. Therefore, the adapta...

Full description

Bibliographic Details
Main Authors:	Mohd Omar, Suhaila, Zainal Abidin, Zaima Azira, Abdul Malek, Nur Fathiah, Mohd Farouk, Norsyafawati, Zainuddin, Zarina, Khan Chowdhury, Ahmed Jalal
Format:	Article
Language:	English English
Published:	Kulliyyah (Faculty) of Medicine, International Islamic University Malaysia 2016
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/53507/ http://irep.iium.edu.my/53507/ http://irep.iium.edu.my/53507/1/poster_medical_researchsymposium.pdf http://irep.iium.edu.my/53507/7/MRS2016-ABSTRACT-BOOK-108.pdf

id	iium-53507
recordtype	eprints
spelling	iium-535072017-12-06T05:03:40Z http://irep.iium.edu.my/53507/ Biotechnological potential of Kuantan mangrove actinomycete, Micromonospora K3-13 Mohd Omar, Suhaila Zainal Abidin, Zaima Azira Abdul Malek, Nur Fathiah Mohd Farouk, Norsyafawati Zainuddin, Zarina Khan Chowdhury, Ahmed Jalal TP248.13 Biotechnology Introduction Mangrove is a complex and unique ecosystem which experiences periodical tidal flooding inundated with low, moderate and high saline water. The muddy soil is rich in organic matter derived from efficient nutrient cycling system and decomposition of mangrove leaves. Therefore, the adaptation of mangrove microorganisms towards these environmental factors could promote the production of interesting bioactive compounds and enzymes. Methodologies This study aimed to unravel the identity and biotechnological potential of a Kuantan mangrove actinomycete, strain K3-13, through morphology observation, molecular identification, antimicrobial test and xylan degradation test. Results The study revealed that this orange-coloured mycelium-forming isolate produced spores after long incubation period which showed characteristics that resembled Micromonaspora colonies. The phylogenetic analysis of the 16S rRNA gene sequence of strain K3-13 indicated that the highest similarity was to Micromonospora carbonacea DSM43168 (99%). Besides, K3-13 produced blue diffusible pigment upon cultivation on three different starch-agar medium. Cross streak assay conducted to detect antimicrobial activities indicated that K1-13 has strong antibacterial activities against Bacillus subtilis and Staphylococcus aureus. K3-13 also showed positive xylanase activities by exhibiting decolourization zone in the agar-based assay using azo-xylan as substrate. Conclusion Nevertheless, it can be concluded that Micromonospora K3-13 could be explored for its potential biotechnological application such as natural dye, antibacterial drugs and xylan biodegraders. Kulliyyah (Faculty) of Medicine, International Islamic University Malaysia 2016 Article PeerReviewed application/pdf en http://irep.iium.edu.my/53507/1/poster_medical_researchsymposium.pdf application/pdf en http://irep.iium.edu.my/53507/7/MRS2016-ABSTRACT-BOOK-108.pdf Mohd Omar, Suhaila and Zainal Abidin, Zaima Azira and Abdul Malek, Nur Fathiah and Mohd Farouk, Norsyafawati and Zainuddin, Zarina and Khan Chowdhury, Ahmed Jalal (2016) Biotechnological potential of Kuantan mangrove actinomycete, Micromonospora K3-13. The International Medical Journal Malaysia, 15 (Supplement Issue). p. 108. ISSN 1823-4631 http://iiumedic.net/imjm/v1/download/Volume%2015%20Supplement/MRS2016-ABSTRACT-BOOK-108.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Mohd Omar, Suhaila Zainal Abidin, Zaima Azira Abdul Malek, Nur Fathiah Mohd Farouk, Norsyafawati Zainuddin, Zarina Khan Chowdhury, Ahmed Jalal Biotechnological potential of Kuantan mangrove actinomycete, Micromonospora K3-13
description	Introduction Mangrove is a complex and unique ecosystem which experiences periodical tidal flooding inundated with low, moderate and high saline water. The muddy soil is rich in organic matter derived from efficient nutrient cycling system and decomposition of mangrove leaves. Therefore, the adaptation of mangrove microorganisms towards these environmental factors could promote the production of interesting bioactive compounds and enzymes. Methodologies This study aimed to unravel the identity and biotechnological potential of a Kuantan mangrove actinomycete, strain K3-13, through morphology observation, molecular identification, antimicrobial test and xylan degradation test. Results The study revealed that this orange-coloured mycelium-forming isolate produced spores after long incubation period which showed characteristics that resembled Micromonaspora colonies. The phylogenetic analysis of the 16S rRNA gene sequence of strain K3-13 indicated that the highest similarity was to Micromonospora carbonacea DSM43168 (99%). Besides, K3-13 produced blue diffusible pigment upon cultivation on three different starch-agar medium. Cross streak assay conducted to detect antimicrobial activities indicated that K1-13 has strong antibacterial activities against Bacillus subtilis and Staphylococcus aureus. K3-13 also showed positive xylanase activities by exhibiting decolourization zone in the agar-based assay using azo-xylan as substrate. Conclusion Nevertheless, it can be concluded that Micromonospora K3-13 could be explored for its potential biotechnological application such as natural dye, antibacterial drugs and xylan biodegraders.
format	Article
author	Mohd Omar, Suhaila Zainal Abidin, Zaima Azira Abdul Malek, Nur Fathiah Mohd Farouk, Norsyafawati Zainuddin, Zarina Khan Chowdhury, Ahmed Jalal
author_facet	Mohd Omar, Suhaila Zainal Abidin, Zaima Azira Abdul Malek, Nur Fathiah Mohd Farouk, Norsyafawati Zainuddin, Zarina Khan Chowdhury, Ahmed Jalal
author_sort	Mohd Omar, Suhaila
title	Biotechnological potential of Kuantan mangrove actinomycete, Micromonospora K3-13
title_short	Biotechnological potential of Kuantan mangrove actinomycete, Micromonospora K3-13
title_full	Biotechnological potential of Kuantan mangrove actinomycete, Micromonospora K3-13
title_fullStr	Biotechnological potential of Kuantan mangrove actinomycete, Micromonospora K3-13
title_full_unstemmed	Biotechnological potential of Kuantan mangrove actinomycete, Micromonospora K3-13
title_sort	biotechnological potential of kuantan mangrove actinomycete, micromonospora k3-13
publisher	Kulliyyah (Faculty) of Medicine, International Islamic University Malaysia
publishDate	2016
url	http://irep.iium.edu.my/53507/ http://irep.iium.edu.my/53507/ http://irep.iium.edu.my/53507/1/poster_medical_researchsymposium.pdf http://irep.iium.edu.my/53507/7/MRS2016-ABSTRACT-BOOK-108.pdf
first_indexed	2023-09-18T21:15:41Z
last_indexed	2023-09-18T21:15:41Z
_version_	1777411546509475840

Biotechnological potential of Kuantan mangrove actinomycete, Micromonospora K3-13

Similar Items