Biotechnological potential of Kuantan mangrove actinomycete, Micromonospora K3-13
Introduction Mangrove is a complex and unique ecosystem which experiences periodical tidal flooding inundated with low, moderate and high saline water. The muddy soil is rich in organic matter derived from efficient nutrient cycling system and decomposition of mangrove leaves. Therefore, the adapta...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Kulliyyah (Faculty) of Medicine, International Islamic University Malaysia
2016
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/53507/ http://irep.iium.edu.my/53507/ http://irep.iium.edu.my/53507/1/poster_medical_researchsymposium.pdf http://irep.iium.edu.my/53507/7/MRS2016-ABSTRACT-BOOK-108.pdf |
| Summary: | Introduction
Mangrove is a complex and unique ecosystem which experiences periodical tidal flooding inundated with low, moderate and high saline water. The muddy soil is rich in organic matter derived from efficient nutrient cycling system and decomposition of mangrove leaves. Therefore, the adaptation of mangrove microorganisms towards these environmental factors could promote the production of interesting bioactive compounds and enzymes.
Methodologies
This study aimed to unravel the identity and biotechnological potential of a Kuantan mangrove actinomycete, strain K3-13, through morphology observation, molecular identification, antimicrobial test and xylan degradation test.
Results
The study revealed that this orange-coloured mycelium-forming isolate produced spores after long incubation period which showed characteristics that resembled Micromonaspora colonies. The phylogenetic analysis of the 16S rRNA gene sequence of strain K3-13 indicated that the highest similarity was to Micromonospora carbonacea DSM43168 (99%). Besides, K3-13 produced blue diffusible pigment upon cultivation on three different starch-agar medium. Cross streak assay conducted to detect antimicrobial activities indicated that K1-13 has strong antibacterial activities against Bacillus subtilis and Staphylococcus aureus. K3-13 also showed positive xylanase activities by exhibiting decolourization zone in the agar-based assay using azo-xylan as substrate.
Conclusion
Nevertheless, it can be concluded that Micromonospora K3-13 could be explored for its potential biotechnological application such as natural dye, antibacterial drugs and xylan biodegraders. |
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