Co-encapsulation of Nigella sativa oil and plasmid DNA for enhanced gene therapy of Alzheimer’s disease

Alzheimer disease involves genetic and non-genetic factors and hence it is rational to be treated with genetic and non-genetic therapeutic agents. Nigella sativa has multiple therapeutic properties including neuroregeneration. Nigella sativa oil (NSO) was encapsulated in PLGA nanoparticles and pDNA...

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Main Authors:	Doolaanea, Abd Almonem, Mansor, Nur 'Izzati, Mohd Nor, Nurul Hafizah, Mohamed, Farahidah
Format:	Article
Language:	English English English
Published:	TAYLOR & FRANCIS LTD 2016
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/53282/ http://irep.iium.edu.my/53282/ http://irep.iium.edu.my/53282/ http://irep.iium.edu.my/53282/1/53282_Co-encapsulation.pdf http://irep.iium.edu.my/53282/2/53282_Co-encapsulation_WOS.pdf http://irep.iium.edu.my/53282/13/53282_Co-encapsulation%20of%20Nigella%20sativa_SCOPUS.pdf

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recordtype	eprints
spelling	iium-532822017-04-05T13:25:16Z http://irep.iium.edu.my/53282/ Co-encapsulation of Nigella sativa oil and plasmid DNA for enhanced gene therapy of Alzheimer’s disease Doolaanea, Abd Almonem Mansor, Nur 'Izzati Mohd Nor, Nurul Hafizah Mohamed, Farahidah RS Pharmacy and materia medica Alzheimer disease involves genetic and non-genetic factors and hence it is rational to be treated with genetic and non-genetic therapeutic agents. Nigella sativa has multiple therapeutic properties including neuroregeneration. Nigella sativa oil (NSO) was encapsulated in PLGA nanoparticles and pDNA was loaded either by adsorption on chitosan-modified particles or encapsulation within PLGA nanoparticles. The particle size and zeta potential of NSO-pDNA-chitosan-PLGA nanoparticles were highly dependent on the medium and exhibited high burst release. Meanwhile, NSO-pDNA-PLGA nanoparticles were more consistent with lower burst release. The fabricated nanoparticles revealed the expected outcomes of both pDNA and NSO. The pDNA transfected N2a cell while the encapsulated NSO promoted neurite outgrowth that is crucial for neuroregeneration. Results from this study suggest that NSO could be added to the gene delivery carrier to enhance treatment benefits for Alzheimer disease. TAYLOR & FRANCIS LTD 2016-02-17 Article PeerReviewed application/pdf en http://irep.iium.edu.my/53282/1/53282_Co-encapsulation.pdf application/pdf en http://irep.iium.edu.my/53282/2/53282_Co-encapsulation_WOS.pdf application/pdf en http://irep.iium.edu.my/53282/13/53282_Co-encapsulation%20of%20Nigella%20sativa_SCOPUS.pdf Doolaanea, Abd Almonem and Mansor, Nur 'Izzati and Mohd Nor, Nurul Hafizah and Mohamed, Farahidah (2016) Co-encapsulation of Nigella sativa oil and plasmid DNA for enhanced gene therapy of Alzheimer’s disease. Journal of Microencapsulation, 33 (2). pp. 114-126. ISSN 0265-2048 E-ISSN 1464-5246 http://www.tandfonline.com/toc/imnc20/curren 10.3109/02652048.2015.1134689
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Doolaanea, Abd Almonem Mansor, Nur 'Izzati Mohd Nor, Nurul Hafizah Mohamed, Farahidah Co-encapsulation of Nigella sativa oil and plasmid DNA for enhanced gene therapy of Alzheimer’s disease
description	Alzheimer disease involves genetic and non-genetic factors and hence it is rational to be treated with genetic and non-genetic therapeutic agents. Nigella sativa has multiple therapeutic properties including neuroregeneration. Nigella sativa oil (NSO) was encapsulated in PLGA nanoparticles and pDNA was loaded either by adsorption on chitosan-modified particles or encapsulation within PLGA nanoparticles. The particle size and zeta potential of NSO-pDNA-chitosan-PLGA nanoparticles were highly dependent on the medium and exhibited high burst release. Meanwhile, NSO-pDNA-PLGA nanoparticles were more consistent with lower burst release. The fabricated nanoparticles revealed the expected outcomes of both pDNA and NSO. The pDNA transfected N2a cell while the encapsulated NSO promoted neurite outgrowth that is crucial for neuroregeneration. Results from this study suggest that NSO could be added to the gene delivery carrier to enhance treatment benefits for Alzheimer disease.
format	Article
author	Doolaanea, Abd Almonem Mansor, Nur 'Izzati Mohd Nor, Nurul Hafizah Mohamed, Farahidah
author_facet	Doolaanea, Abd Almonem Mansor, Nur 'Izzati Mohd Nor, Nurul Hafizah Mohamed, Farahidah
author_sort	Doolaanea, Abd Almonem
title	Co-encapsulation of Nigella sativa oil and plasmid DNA for enhanced gene therapy of Alzheimer’s disease
title_short	Co-encapsulation of Nigella sativa oil and plasmid DNA for enhanced gene therapy of Alzheimer’s disease
title_full	Co-encapsulation of Nigella sativa oil and plasmid DNA for enhanced gene therapy of Alzheimer’s disease
title_fullStr	Co-encapsulation of Nigella sativa oil and plasmid DNA for enhanced gene therapy of Alzheimer’s disease
title_full_unstemmed	Co-encapsulation of Nigella sativa oil and plasmid DNA for enhanced gene therapy of Alzheimer’s disease
title_sort	co-encapsulation of nigella sativa oil and plasmid dna for enhanced gene therapy of alzheimer’s disease
publisher	TAYLOR & FRANCIS LTD
publishDate	2016
url	http://irep.iium.edu.my/53282/ http://irep.iium.edu.my/53282/ http://irep.iium.edu.my/53282/ http://irep.iium.edu.my/53282/1/53282_Co-encapsulation.pdf http://irep.iium.edu.my/53282/2/53282_Co-encapsulation_WOS.pdf http://irep.iium.edu.my/53282/13/53282_Co-encapsulation%20of%20Nigella%20sativa_SCOPUS.pdf
first_indexed	2023-09-18T21:15:25Z
last_indexed	2023-09-18T21:15:25Z
_version_	1777411530102407168

Co-encapsulation of Nigella sativa oil and plasmid DNA for enhanced gene therapy of Alzheimer’s disease

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