Effects of flaxseed extract on the viability of stem cells from human exfoliated deciduous teeth

Purpose: To determine the effects different concentrations of flaxseed crude extract on the viability of stem cells from human exfoliated deciduous teeth (SHED). Methods: Whole flaxseeds were ground and extracted using 99.8% ethanol in a Soxhlet chamber. The crude extract was kept in a closed cont...

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Main Authors:	Mokhtar@Makhtar, Khairani Idah, Nordin, Nur Sazwi, Mustafa Al-Ahmad, Basma Ezzat, Lestari, Widya, Arief Ichwan, Solachuddin Jauhari, Darnis, Deny Susanti, Kannan, Thirumulu Ponnuraj
Format:	Conference or Workshop Item
Language:	English
Published:	2016
Subjects:	RK Dentistry
Online Access:	http://irep.iium.edu.my/53257/ http://irep.iium.edu.my/53257/ http://irep.iium.edu.my/53257/1/53257_Effects%20of%20flaxseed%20extract.pdf

id	iium-53257
recordtype	eprints
spelling	iium-532572017-03-07T03:29:19Z http://irep.iium.edu.my/53257/ Effects of flaxseed extract on the viability of stem cells from human exfoliated deciduous teeth Mokhtar@Makhtar, Khairani Idah Nordin, Nur Sazwi Mustafa Al-Ahmad, Basma Ezzat Lestari, Widya Arief Ichwan, Solachuddin Jauhari Darnis, Deny Susanti Kannan, Thirumulu Ponnuraj RK Dentistry Purpose: To determine the effects different concentrations of flaxseed crude extract on the viability of stem cells from human exfoliated deciduous teeth (SHED). Methods: Whole flaxseeds were ground and extracted using 99.8% ethanol in a Soxhlet chamber. The crude extract was kept in a closed container and stored at 4°C until further use. SHED (ALLCells, USA) was cultured and maintained in Alpha-MEM supplemented with FBS and antibiotics at 37°C in a CO2 incubator. Cells were trypsinised and 1000 cells were seeded in a 96-well plate. After 24 hours of incubation, SHED were treated with different concentrations of Flaxseed crude extract (0.0-16 mg/ml). The cell viability was determined using MTT assay after 3 days’ incubation. The morphology of the cells was also observed. Results: The SHED viability was more than 100% until a concentration of 8 mg/ml of flaxseed extract and an IC50 and IC25 values at 11.85 mg/ml and 10.82 mg/ml respectively which lead to cell death. Additionally, there were also morphological changes when treated with these different concentrations. Cells appeared healthy and maintained the fibroblast-like shape at 4 mg/ml. However, at 8 mg/ml, some of the cells cytoplasm started to demonstrate vacuolation and granulation and at 16 mg/ml, all cells appeared non-viable. Conclusions: The cell viability of SHED was more than 100% until 8 mg/ml of the flaxseed extract consistent with the morphological changes indicating the potential of this extract to be used further in research relating to tissue engineering involving stem cells. 2016-11-17 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/53257/1/53257_Effects%20of%20flaxseed%20extract.pdf Mokhtar@Makhtar, Khairani Idah and Nordin, Nur Sazwi and Mustafa Al-Ahmad, Basma Ezzat and Lestari, Widya and Arief Ichwan, Solachuddin Jauhari and Darnis, Deny Susanti and Kannan, Thirumulu Ponnuraj (2016) Effects of flaxseed extract on the viability of stem cells from human exfoliated deciduous teeth. In: 6th MTERMS (Malaysian Tissue Engineering and Regenerative Medicine Scientific Meeting) 2016 in conjunction with 2nd Malaysian Stem Cell Meeting, 17th-18th Nov. 2016, Seberang Jaya, Pulau Pinang. (Unpublished) http://mterms.tesma.org.my/wp-content/uploads/2016/11/Abstract-6th-MTERMS-2016.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RK Dentistry
spellingShingle	RK Dentistry Mokhtar@Makhtar, Khairani Idah Nordin, Nur Sazwi Mustafa Al-Ahmad, Basma Ezzat Lestari, Widya Arief Ichwan, Solachuddin Jauhari Darnis, Deny Susanti Kannan, Thirumulu Ponnuraj Effects of flaxseed extract on the viability of stem cells from human exfoliated deciduous teeth
description	Purpose: To determine the effects different concentrations of flaxseed crude extract on the viability of stem cells from human exfoliated deciduous teeth (SHED). Methods: Whole flaxseeds were ground and extracted using 99.8% ethanol in a Soxhlet chamber. The crude extract was kept in a closed container and stored at 4°C until further use. SHED (ALLCells, USA) was cultured and maintained in Alpha-MEM supplemented with FBS and antibiotics at 37°C in a CO2 incubator. Cells were trypsinised and 1000 cells were seeded in a 96-well plate. After 24 hours of incubation, SHED were treated with different concentrations of Flaxseed crude extract (0.0-16 mg/ml). The cell viability was determined using MTT assay after 3 days’ incubation. The morphology of the cells was also observed. Results: The SHED viability was more than 100% until a concentration of 8 mg/ml of flaxseed extract and an IC50 and IC25 values at 11.85 mg/ml and 10.82 mg/ml respectively which lead to cell death. Additionally, there were also morphological changes when treated with these different concentrations. Cells appeared healthy and maintained the fibroblast-like shape at 4 mg/ml. However, at 8 mg/ml, some of the cells cytoplasm started to demonstrate vacuolation and granulation and at 16 mg/ml, all cells appeared non-viable. Conclusions: The cell viability of SHED was more than 100% until 8 mg/ml of the flaxseed extract consistent with the morphological changes indicating the potential of this extract to be used further in research relating to tissue engineering involving stem cells.
format	Conference or Workshop Item
author	Mokhtar@Makhtar, Khairani Idah Nordin, Nur Sazwi Mustafa Al-Ahmad, Basma Ezzat Lestari, Widya Arief Ichwan, Solachuddin Jauhari Darnis, Deny Susanti Kannan, Thirumulu Ponnuraj
author_facet	Mokhtar@Makhtar, Khairani Idah Nordin, Nur Sazwi Mustafa Al-Ahmad, Basma Ezzat Lestari, Widya Arief Ichwan, Solachuddin Jauhari Darnis, Deny Susanti Kannan, Thirumulu Ponnuraj
author_sort	Mokhtar@Makhtar, Khairani Idah
title	Effects of flaxseed extract on the viability of stem cells from human exfoliated deciduous teeth
title_short	Effects of flaxseed extract on the viability of stem cells from human exfoliated deciduous teeth
title_full	Effects of flaxseed extract on the viability of stem cells from human exfoliated deciduous teeth
title_fullStr	Effects of flaxseed extract on the viability of stem cells from human exfoliated deciduous teeth
title_full_unstemmed	Effects of flaxseed extract on the viability of stem cells from human exfoliated deciduous teeth
title_sort	effects of flaxseed extract on the viability of stem cells from human exfoliated deciduous teeth
publishDate	2016
url	http://irep.iium.edu.my/53257/ http://irep.iium.edu.my/53257/ http://irep.iium.edu.my/53257/1/53257_Effects%20of%20flaxseed%20extract.pdf
first_indexed	2023-09-18T21:15:24Z
last_indexed	2023-09-18T21:15:24Z
_version_	1777411528794832896

Effects of flaxseed extract on the viability of stem cells from human exfoliated deciduous teeth

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