Antioxidant and antimicrobial properties of Tinospora Crispa (Putarwali) stems methanolic extract

Apart from being the primary source of food to other living things, plants also have medicinal value to treat various kinds of diseases. In recent years, it has been proposed that the extract from plants may be used as natural antioxidants which can help to prevent the generation of carcinogens in h...

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Main Authors:	Sulaiman, Fatimatul Akmal, Fuad, Nurfarahin, Rahman, Farawahida, Iqbal, Anwar, Darnis, Deny Susanti
Format:	Article
Language:	English English
Published:	Universiti Teknologi Malaysia 2016
Subjects:	QD Chemistry
Online Access:	http://irep.iium.edu.my/53160/ http://irep.iium.edu.my/53160/ http://irep.iium.edu.my/53160/ http://irep.iium.edu.my/53160/1/Tinospora9941-27851-2-PB.pdf http://irep.iium.edu.my/53160/7/53160_antioxidant%20and%20antimicrobial_scopus.pdf

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spelling	iium-531602017-04-03T03:28:52Z http://irep.iium.edu.my/53160/ Antioxidant and antimicrobial properties of Tinospora Crispa (Putarwali) stems methanolic extract Sulaiman, Fatimatul Akmal Fuad, Nurfarahin Rahman, Farawahida Iqbal, Anwar Darnis, Deny Susanti QD Chemistry Apart from being the primary source of food to other living things, plants also have medicinal value to treat various kinds of diseases. In recent years, it has been proposed that the extract from plants may be used as natural antioxidants which can help to prevent the generation of carcinogens in human body. In addition, plants also have antimicrobial agents to inhibit the growth of pathogenic microbes. This study was intended to investigate the antioxidant properties and antimicrobial activity of methanolic extract of Tinospora crispa stems extracted using soxhlet extraction method. The antimicrobial properties of T. crispa stems extract were tested using disc diffusion method against Staphylococcus aureus ATCC 25923, Bacillus cereus ATCC 11778, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Candida albicans IMR C S23/11 A and Saccharomyces cerevisiae IMR S 617/068. The antioxidant properties of the extract were investigated by using Total Phenolics Content (TPC), Total Flavonoids Content (TFC), DPPH free radical scavenging and –carotene bleaching assays. The TPC value was 6.12 g GAE/100 g of dried extract while the TFC value was 55.58 g QE/100 g of dried extract. The IC50 of DPPH scavenging assay for the extract and ascorbic acid were 0.21 and 0.04 mg/mL, respectively. The average percentage of – carotene bleaching assay was 38.3 % as compared to BHT, which was 45.1%. The disc diffusion method showed no inhibition zone against all the strains of microorganisms at all concentrations of the extracts (0.5, 1.0, 2.5 and 5.0 mg/disc). Universiti Teknologi Malaysia 2016 Article PeerReviewed application/pdf en http://irep.iium.edu.my/53160/1/Tinospora9941-27851-2-PB.pdf application/pdf en http://irep.iium.edu.my/53160/7/53160_antioxidant%20and%20antimicrobial_scopus.pdf Sulaiman, Fatimatul Akmal and Fuad, Nurfarahin and Rahman, Farawahida and Iqbal, Anwar and Darnis, Deny Susanti (2016) Antioxidant and antimicrobial properties of Tinospora Crispa (Putarwali) stems methanolic extract. Jurnal Teknologi, 78 (11). pp. 35-40. E-ISSN 21803722 http://www.jurnalteknologi.utm.my/index.php/jurnalteknologi/article/view/9941/5979 10.11113/jt.v78.9941
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	QD Chemistry
spellingShingle	QD Chemistry Sulaiman, Fatimatul Akmal Fuad, Nurfarahin Rahman, Farawahida Iqbal, Anwar Darnis, Deny Susanti Antioxidant and antimicrobial properties of Tinospora Crispa (Putarwali) stems methanolic extract
description	Apart from being the primary source of food to other living things, plants also have medicinal value to treat various kinds of diseases. In recent years, it has been proposed that the extract from plants may be used as natural antioxidants which can help to prevent the generation of carcinogens in human body. In addition, plants also have antimicrobial agents to inhibit the growth of pathogenic microbes. This study was intended to investigate the antioxidant properties and antimicrobial activity of methanolic extract of Tinospora crispa stems extracted using soxhlet extraction method. The antimicrobial properties of T. crispa stems extract were tested using disc diffusion method against Staphylococcus aureus ATCC 25923, Bacillus cereus ATCC 11778, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Candida albicans IMR C S23/11 A and Saccharomyces cerevisiae IMR S 617/068. The antioxidant properties of the extract were investigated by using Total Phenolics Content (TPC), Total Flavonoids Content (TFC), DPPH free radical scavenging and –carotene bleaching assays. The TPC value was 6.12 g GAE/100 g of dried extract while the TFC value was 55.58 g QE/100 g of dried extract. The IC50 of DPPH scavenging assay for the extract and ascorbic acid were 0.21 and 0.04 mg/mL, respectively. The average percentage of – carotene bleaching assay was 38.3 % as compared to BHT, which was 45.1%. The disc diffusion method showed no inhibition zone against all the strains of microorganisms at all concentrations of the extracts (0.5, 1.0, 2.5 and 5.0 mg/disc).
format	Article
author	Sulaiman, Fatimatul Akmal Fuad, Nurfarahin Rahman, Farawahida Iqbal, Anwar Darnis, Deny Susanti
author_facet	Sulaiman, Fatimatul Akmal Fuad, Nurfarahin Rahman, Farawahida Iqbal, Anwar Darnis, Deny Susanti
author_sort	Sulaiman, Fatimatul Akmal
title	Antioxidant and antimicrobial properties of Tinospora Crispa (Putarwali) stems methanolic extract
title_short	Antioxidant and antimicrobial properties of Tinospora Crispa (Putarwali) stems methanolic extract
title_full	Antioxidant and antimicrobial properties of Tinospora Crispa (Putarwali) stems methanolic extract
title_fullStr	Antioxidant and antimicrobial properties of Tinospora Crispa (Putarwali) stems methanolic extract
title_full_unstemmed	Antioxidant and antimicrobial properties of Tinospora Crispa (Putarwali) stems methanolic extract
title_sort	antioxidant and antimicrobial properties of tinospora crispa (putarwali) stems methanolic extract
publisher	Universiti Teknologi Malaysia
publishDate	2016
url	http://irep.iium.edu.my/53160/ http://irep.iium.edu.my/53160/ http://irep.iium.edu.my/53160/ http://irep.iium.edu.my/53160/1/Tinospora9941-27851-2-PB.pdf http://irep.iium.edu.my/53160/7/53160_antioxidant%20and%20antimicrobial_scopus.pdf
first_indexed	2023-09-18T21:15:15Z
last_indexed	2023-09-18T21:15:15Z
_version_	1777411519515983872

Antioxidant and antimicrobial properties of Tinospora Crispa (Putarwali) stems methanolic extract

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