Marine streptomyces SP. UKMCC_PT15 producing undecylprodigiosin with algicidal activity

Marine actinomycetes are now in demand as they are capable of producing unique and novel compounds with wide biological activities. Marine Streptomyces sp. UKMCC_PT15 previously isolated from seawater collected in Pulau Tinggi, Johor was used in this study. Molecular identification showed high si...

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Main Authors:	Zainal Abidin, Zaima Azira, Ahmad, Asmat, Latip, Jalifah, Usup, Gires
Format:	Article
Language:	English
Published:	UTM 2016
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/53101/ http://irep.iium.edu.my/53101/ http://irep.iium.edu.my/53101/1/jurnal%20teknologi%20%202016.pdf

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spelling	iium-531012016-12-29T02:47:18Z http://irep.iium.edu.my/53101/ Marine streptomyces SP. UKMCC_PT15 producing undecylprodigiosin with algicidal activity Zainal Abidin, Zaima Azira Ahmad, Asmat Latip, Jalifah Usup, Gires Q Science (General) Marine actinomycetes are now in demand as they are capable of producing unique and novel compounds with wide biological activities. Marine Streptomyces sp. UKMCC_PT15 previously isolated from seawater collected in Pulau Tinggi, Johor was used in this study. Molecular identification showed high similarity of Streptomyces sp. UKMCC_PT15 with Streptomyces fradiae and S. diastaticus subsp. ardesiacus based on partial sequence of 16S rRNA gene. Further characterisations of this bacterium include spore morphology using SEM, growth on various media, salt tolerance test and carbon utilisation profile. This bacterium had straight spore chain with smooth-surfaced spores and was able to tolerate up to 11% NaCl with capabilities of utilising >40 carbon sources. Undecylprodigiosin (C25H35N3O) was successfully purified through succession of column chromatography and finally using HPLC. Structure elucidation was confirmed through NMR spectroscopy, MS and comparison with established data. This compound demonstrated strong antibacterial activities against S. aureus, B. subtilis and C. albicans but weak antibacterial activities against E. coli, P. aeruginosa and Methicillin-resistant S. aureus (MRSA), Interestingly, undecylprodigiosin also demonstrated algicidal activity when tested against toxic dinoflagellates, A. minutum and P. bahamense, both of which are responsible for harmful algal blooms (HABs). Undecylprodiogiosin with concentration of 10-100 μg/ml gave ~100% algicidal activity against both dinoflagellates. Further testing with undecylprodigiosin concentration < 10 µg/ml showed that undecylprodigision was capable of killing significantly high numbers of both dinoflagellates, giving a high algicidal activity. Findings from this study suggested the potential use of undecylprodigiosin as algicidal agent which could be used for the mitigation of HABs. UTM 2016-11-21 Article PeerReviewed application/pdf en http://irep.iium.edu.my/53101/1/jurnal%20teknologi%20%202016.pdf Zainal Abidin, Zaima Azira and Ahmad, Asmat and Latip, Jalifah and Usup, Gires (2016) Marine streptomyces SP. UKMCC_PT15 producing undecylprodigiosin with algicidal activity. Jurnal Teknologi, 78 (11(2)). pp. 55-60. E-ISSN 2180-3722 10.11113/jt.v78.9944
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Zainal Abidin, Zaima Azira Ahmad, Asmat Latip, Jalifah Usup, Gires Marine streptomyces SP. UKMCC_PT15 producing undecylprodigiosin with algicidal activity
description	Marine actinomycetes are now in demand as they are capable of producing unique and novel compounds with wide biological activities. Marine Streptomyces sp. UKMCC_PT15 previously isolated from seawater collected in Pulau Tinggi, Johor was used in this study. Molecular identification showed high similarity of Streptomyces sp. UKMCC_PT15 with Streptomyces fradiae and S. diastaticus subsp. ardesiacus based on partial sequence of 16S rRNA gene. Further characterisations of this bacterium include spore morphology using SEM, growth on various media, salt tolerance test and carbon utilisation profile. This bacterium had straight spore chain with smooth-surfaced spores and was able to tolerate up to 11% NaCl with capabilities of utilising >40 carbon sources. Undecylprodigiosin (C25H35N3O) was successfully purified through succession of column chromatography and finally using HPLC. Structure elucidation was confirmed through NMR spectroscopy, MS and comparison with established data. This compound demonstrated strong antibacterial activities against S. aureus, B. subtilis and C. albicans but weak antibacterial activities against E. coli, P. aeruginosa and Methicillin-resistant S. aureus (MRSA), Interestingly, undecylprodigiosin also demonstrated algicidal activity when tested against toxic dinoflagellates, A. minutum and P. bahamense, both of which are responsible for harmful algal blooms (HABs). Undecylprodiogiosin with concentration of 10-100 μg/ml gave ~100% algicidal activity against both dinoflagellates. Further testing with undecylprodigiosin concentration < 10 µg/ml showed that undecylprodigision was capable of killing significantly high numbers of both dinoflagellates, giving a high algicidal activity. Findings from this study suggested the potential use of undecylprodigiosin as algicidal agent which could be used for the mitigation of HABs.
format	Article
author	Zainal Abidin, Zaima Azira Ahmad, Asmat Latip, Jalifah Usup, Gires
author_facet	Zainal Abidin, Zaima Azira Ahmad, Asmat Latip, Jalifah Usup, Gires
author_sort	Zainal Abidin, Zaima Azira
title	Marine streptomyces SP. UKMCC_PT15 producing undecylprodigiosin with algicidal activity
title_short	Marine streptomyces SP. UKMCC_PT15 producing undecylprodigiosin with algicidal activity
title_full	Marine streptomyces SP. UKMCC_PT15 producing undecylprodigiosin with algicidal activity
title_fullStr	Marine streptomyces SP. UKMCC_PT15 producing undecylprodigiosin with algicidal activity
title_full_unstemmed	Marine streptomyces SP. UKMCC_PT15 producing undecylprodigiosin with algicidal activity
title_sort	marine streptomyces sp. ukmcc_pt15 producing undecylprodigiosin with algicidal activity
publisher	UTM
publishDate	2016
url	http://irep.iium.edu.my/53101/ http://irep.iium.edu.my/53101/ http://irep.iium.edu.my/53101/1/jurnal%20teknologi%20%202016.pdf
first_indexed	2023-09-18T21:15:11Z
last_indexed	2023-09-18T21:15:11Z
_version_	1777411514591870976

Marine streptomyces SP. UKMCC_PT15 producing undecylprodigiosin with algicidal activity

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