Antimicrobial activities of actinomycetes isolated from Malaysian tropical mangrove forest of Kuantan, Pahang

Actinomycetes, a group of slow growing, Gram positive bacteria belonging to the order of Actinomycetales are widely distributed in nature. They are biotechnologically important for their renowned capability to produce various bioactive secondary metabolites. Isolation and screening from poorly explo...

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Main Authors:	Zainal Abidin, Zaima Azira, Abdul Malek, Nurfathiah, Chowdhury, Ahmed Jalal Khan, Zainuddin, Zarina
Format:	Conference or Workshop Item
Language:	English
Published:	2016
Subjects:	QR Microbiology
Online Access:	http://irep.iium.edu.my/52964/ http://irep.iium.edu.my/52964/28/52964.pdf

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recordtype	eprints
spelling	iium-529642017-01-05T04:18:37Z http://irep.iium.edu.my/52964/ Antimicrobial activities of actinomycetes isolated from Malaysian tropical mangrove forest of Kuantan, Pahang Zainal Abidin, Zaima Azira Abdul Malek, Nurfathiah Chowdhury, Ahmed Jalal Khan Zainuddin, Zarina QR Microbiology Actinomycetes, a group of slow growing, Gram positive bacteria belonging to the order of Actinomycetales are widely distributed in nature. They are biotechnologically important for their renowned capability to produce various bioactive secondary metabolites. Isolation and screening from poorly explored habitat offers a promising natural source of bioactive actinomycetes. This study aimed to assess the diversity of actinomycetes from mangrove sediments of Kuantan, Pahang and evaluate their potential for the production of antimicrobial secondary metabolites. A total of 100 representative isolates were selected for further investigation from a collection of 3484 isolates recovered from 5 different sampling sites using various isolation procedures. The antimicrobial potential of the representative isolates were preliminary assessed using the cross streak method against Bacillus subtilis, Candida albicans, Escherichia coli, Serratia marcescens and Staphylococcus aureus. Approximately 53.0 % of the total isolates possessed antimicrobial potential against at least one of the test organisms. The crude ethyl acetate extract of these bioactive isolates were further evaluated using disc diffusion method, in which 26.4% (n=14) isolates exhibited inhibitory activity against more than half of the test organisms. Streptomyces sp. K1-01 and Streptomyces sp. K2-03 demonstrated broad spectrum of antimicrobial activity against all test organisms, indicating that genus Streptomyces could provide high quality metabolites for drug discovery. Fourteen selected bioactive isolates were screened for biosynthetic genes encoding the non-ribosomal peptide synthetases (NRPS) and polyketide synthases type I (PKS-I). The NRPS and PKS-I gene were detected in 50.0% and 71.4% of the total isolates, respectively. Six isolates exhibited the presence of both NRPS and PKS-I genes, of which 4 isolates belong to genus Streptomyces and another 2 isolates belong to genus Micromonospora. The occurrence of NRPS and PKS-I genes provides an early evaluation on their biosynthetic capabilities. Mangrove actinomycetes enumerated in this study demonstrated high level of diversity as the 33 isolates chosen for phylogenetic analysis using 16S rRNA gene sequences were distributed among 9 genera. Streptomyces and Micromonospora are the most predominant genera observed; alongside Micrococcus, Gordonia and Nocardia. Besides, this is the first report describing the isolation of Dietzia, Pseudonocardia, Saccharopolyspora and Verrucosispora from Malaysian mangrove sediments. In conclusion, Malaysian mangrove ecosystem has the potential as an important reservoir of actinomycetes for the discovery of antimicrobial secondary metabolites 2016-05 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/52964/28/52964.pdf Zainal Abidin, Zaima Azira and Abdul Malek, Nurfathiah and Chowdhury, Ahmed Jalal Khan and Zainuddin, Zarina (2016) Antimicrobial activities of actinomycetes isolated from Malaysian tropical mangrove forest of Kuantan, Pahang. In: 5th International Conference on Environment, Energy and Biotechnology 2016, 23rd-25th May 2016, Jeju Island, South Korea. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QR Microbiology
spellingShingle	QR Microbiology Zainal Abidin, Zaima Azira Abdul Malek, Nurfathiah Chowdhury, Ahmed Jalal Khan Zainuddin, Zarina Antimicrobial activities of actinomycetes isolated from Malaysian tropical mangrove forest of Kuantan, Pahang
description	Actinomycetes, a group of slow growing, Gram positive bacteria belonging to the order of Actinomycetales are widely distributed in nature. They are biotechnologically important for their renowned capability to produce various bioactive secondary metabolites. Isolation and screening from poorly explored habitat offers a promising natural source of bioactive actinomycetes. This study aimed to assess the diversity of actinomycetes from mangrove sediments of Kuantan, Pahang and evaluate their potential for the production of antimicrobial secondary metabolites. A total of 100 representative isolates were selected for further investigation from a collection of 3484 isolates recovered from 5 different sampling sites using various isolation procedures. The antimicrobial potential of the representative isolates were preliminary assessed using the cross streak method against Bacillus subtilis, Candida albicans, Escherichia coli, Serratia marcescens and Staphylococcus aureus. Approximately 53.0 % of the total isolates possessed antimicrobial potential against at least one of the test organisms. The crude ethyl acetate extract of these bioactive isolates were further evaluated using disc diffusion method, in which 26.4% (n=14) isolates exhibited inhibitory activity against more than half of the test organisms. Streptomyces sp. K1-01 and Streptomyces sp. K2-03 demonstrated broad spectrum of antimicrobial activity against all test organisms, indicating that genus Streptomyces could provide high quality metabolites for drug discovery. Fourteen selected bioactive isolates were screened for biosynthetic genes encoding the non-ribosomal peptide synthetases (NRPS) and polyketide synthases type I (PKS-I). The NRPS and PKS-I gene were detected in 50.0% and 71.4% of the total isolates, respectively. Six isolates exhibited the presence of both NRPS and PKS-I genes, of which 4 isolates belong to genus Streptomyces and another 2 isolates belong to genus Micromonospora. The occurrence of NRPS and PKS-I genes provides an early evaluation on their biosynthetic capabilities. Mangrove actinomycetes enumerated in this study demonstrated high level of diversity as the 33 isolates chosen for phylogenetic analysis using 16S rRNA gene sequences were distributed among 9 genera. Streptomyces and Micromonospora are the most predominant genera observed; alongside Micrococcus, Gordonia and Nocardia. Besides, this is the first report describing the isolation of Dietzia, Pseudonocardia, Saccharopolyspora and Verrucosispora from Malaysian mangrove sediments. In conclusion, Malaysian mangrove ecosystem has the potential as an important reservoir of actinomycetes for the discovery of antimicrobial secondary metabolites
format	Conference or Workshop Item
author	Zainal Abidin, Zaima Azira Abdul Malek, Nurfathiah Chowdhury, Ahmed Jalal Khan Zainuddin, Zarina
author_facet	Zainal Abidin, Zaima Azira Abdul Malek, Nurfathiah Chowdhury, Ahmed Jalal Khan Zainuddin, Zarina
author_sort	Zainal Abidin, Zaima Azira
title	Antimicrobial activities of actinomycetes isolated from Malaysian tropical mangrove forest of Kuantan, Pahang
title_short	Antimicrobial activities of actinomycetes isolated from Malaysian tropical mangrove forest of Kuantan, Pahang
title_full	Antimicrobial activities of actinomycetes isolated from Malaysian tropical mangrove forest of Kuantan, Pahang
title_fullStr	Antimicrobial activities of actinomycetes isolated from Malaysian tropical mangrove forest of Kuantan, Pahang
title_full_unstemmed	Antimicrobial activities of actinomycetes isolated from Malaysian tropical mangrove forest of Kuantan, Pahang
title_sort	antimicrobial activities of actinomycetes isolated from malaysian tropical mangrove forest of kuantan, pahang
publishDate	2016
url	http://irep.iium.edu.my/52964/ http://irep.iium.edu.my/52964/28/52964.pdf
first_indexed	2023-09-18T21:15:00Z
last_indexed	2023-09-18T21:15:00Z
_version_	1777411503485353984

Antimicrobial activities of actinomycetes isolated from Malaysian tropical mangrove forest of Kuantan, Pahang

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