51-Chromiumethylenediaminetetraacetic acid (51Cr-EDTA) glomerular filtration Rate (GFR) analysis: comparing two blood sampling with multiple blood sampling

51-Chromiumethylenediaminetetraacetic acid (51Cr-EDTA) glomerular filtration Rate (GFR) analysis: comparing two blood sampling with multiple blood sampling

The glomerular filtration rate (GFR) is the commonest method used to quantify renal function. Chromium-51-Ethylenediaminetetraaceticacid (51Cr-EDTA) plasma clearance has been accepted as a gold standard method in measuring GFR. Several methods have been proposed in which the glomerular filtration ra...

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Bibliographic Details
Main Author: Abdul Rahim, Mohamad Shahrir
Format: Conference or Workshop Item
Language:English
English
Published: 2015
Subjects:
RC Internal medicine
Online Access:http://irep.iium.edu.my/52642/
http://irep.iium.edu.my/52642/
http://irep.iium.edu.my/52642/1/MRS2015%20Poster%20CrEDTA%20GFR.pdf
http://irep.iium.edu.my/52642/8/List%20of%20presenters%20%E2%80%93%20Medical%20Research%20Symposium%202015.pdf
id iium-52642
recordtype eprints
spelling iium-526422017-03-31T03:55:19Z http://irep.iium.edu.my/52642/ 51-Chromiumethylenediaminetetraacetic acid (51Cr-EDTA) glomerular filtration Rate (GFR) analysis: comparing two blood sampling with multiple blood sampling Abdul Rahim, Mohamad Shahrir RC Internal medicine The glomerular filtration rate (GFR) is the commonest method used to quantify renal function. Chromium-51-Ethylenediaminetetraaceticacid (51Cr-EDTA) plasma clearance has been accepted as a gold standard method in measuring GFR. Several methods have been proposed in which the glomerular filtration rate is estimated from using only one or two blood samples to using multiple blood samples. The purpose of this study is to determine the accuracy of using two blood samples compared to multiple blood samples, for calculation of GFR by the 51Cr-EDTA method. In addition, the study aimed to determine the best combination time of two blood sampling and correlation with gender and ethnicity of the patients. 51Cr-EDTA tracer solution is injected into a vein in the upper arm. Six mls of whole blood is then drawn from the opposite arm at 120, 150, 180 and 240 minutes, centrifuged and 2 ml aliquots of plasma are pipetted and counted for a period of 5 minute each time, in an automatic gamma well counter. GFR is calculated by measuring Cr-EDTA plasma clearance using slope intercept method. The patients’ height and weight is used to correct the GFR to body surface area. Correlations of the values are tested by Pearson and Intraclass correlation, and mean and standard deviation derived from pair t-test. All time point combinations using two blood sampling shows a high correlation with multiple blood sampling method (p value < 0.001, r = 0.909 to 0.989). The best combination using two blood sampling is at 2 and 4 hour time point (r = 0.989 with 99% consistency). 51Cr-EDTA GFR estimation using two blood sampling method is accurate and reliable. The two and four hour time point is found to be the best. Gender and ethnicity does not influence GFR estimation using two blood sampling method. 2015-12-10 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/52642/1/MRS2015%20Poster%20CrEDTA%20GFR.pdf application/pdf en http://irep.iium.edu.my/52642/8/List%20of%20presenters%20%E2%80%93%20Medical%20Research%20Symposium%202015.pdf Abdul Rahim, Mohamad Shahrir (2015) 51-Chromiumethylenediaminetetraacetic acid (51Cr-EDTA) glomerular filtration Rate (GFR) analysis: comparing two blood sampling with multiple blood sampling. In: Medical Research Symposium 2015, 10th Dec. 2015, Kuantan, Pahang. http://iiumedic.net/mrs2015/programme/medical-research-symposium-2015/list-of-presenters/
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
English
topic RC Internal medicine
spellingShingle RC Internal medicine
Abdul Rahim, Mohamad Shahrir
51-Chromiumethylenediaminetetraacetic acid (51Cr-EDTA) glomerular filtration Rate (GFR) analysis: comparing two blood sampling with multiple blood sampling
description The glomerular filtration rate (GFR) is the commonest method used to quantify renal function. Chromium-51-Ethylenediaminetetraaceticacid (51Cr-EDTA) plasma clearance has been accepted as a gold standard method in measuring GFR. Several methods have been proposed in which the glomerular filtration rate is estimated from using only one or two blood samples to using multiple blood samples. The purpose of this study is to determine the accuracy of using two blood samples compared to multiple blood samples, for calculation of GFR by the 51Cr-EDTA method. In addition, the study aimed to determine the best combination time of two blood sampling and correlation with gender and ethnicity of the patients. 51Cr-EDTA tracer solution is injected into a vein in the upper arm. Six mls of whole blood is then drawn from the opposite arm at 120, 150, 180 and 240 minutes, centrifuged and 2 ml aliquots of plasma are pipetted and counted for a period of 5 minute each time, in an automatic gamma well counter. GFR is calculated by measuring Cr-EDTA plasma clearance using slope intercept method. The patients’ height and weight is used to correct the GFR to body surface area. Correlations of the values are tested by Pearson and Intraclass correlation, and mean and standard deviation derived from pair t-test. All time point combinations using two blood sampling shows a high correlation with multiple blood sampling method (p value < 0.001, r = 0.909 to 0.989). The best combination using two blood sampling is at 2 and 4 hour time point (r = 0.989 with 99% consistency). 51Cr-EDTA GFR estimation using two blood sampling method is accurate and reliable. The two and four hour time point is found to be the best. Gender and ethnicity does not influence GFR estimation using two blood sampling method.
format Conference or Workshop Item
author Abdul Rahim, Mohamad Shahrir
author_facet Abdul Rahim, Mohamad Shahrir
author_sort Abdul Rahim, Mohamad Shahrir
title 51-Chromiumethylenediaminetetraacetic acid (51Cr-EDTA) glomerular filtration Rate (GFR) analysis: comparing two blood sampling with multiple blood sampling
title_short 51-Chromiumethylenediaminetetraacetic acid (51Cr-EDTA) glomerular filtration Rate (GFR) analysis: comparing two blood sampling with multiple blood sampling
title_full 51-Chromiumethylenediaminetetraacetic acid (51Cr-EDTA) glomerular filtration Rate (GFR) analysis: comparing two blood sampling with multiple blood sampling
title_fullStr 51-Chromiumethylenediaminetetraacetic acid (51Cr-EDTA) glomerular filtration Rate (GFR) analysis: comparing two blood sampling with multiple blood sampling
title_full_unstemmed 51-Chromiumethylenediaminetetraacetic acid (51Cr-EDTA) glomerular filtration Rate (GFR) analysis: comparing two blood sampling with multiple blood sampling
title_sort 51-chromiumethylenediaminetetraacetic acid (51cr-edta) glomerular filtration rate (gfr) analysis: comparing two blood sampling with multiple blood sampling
publishDate 2015
url http://irep.iium.edu.my/52642/
http://irep.iium.edu.my/52642/
http://irep.iium.edu.my/52642/1/MRS2015%20Poster%20CrEDTA%20GFR.pdf
http://irep.iium.edu.my/52642/8/List%20of%20presenters%20%E2%80%93%20Medical%20Research%20Symposium%202015.pdf
first_indexed 2023-09-18T21:14:33Z
last_indexed 2023-09-18T21:14:33Z
_version_ 1777411475101450240

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