Screening of the factors for hydrogel synthesis using design-of-experiment

The two level factorial design was chosen from design experiment software version 8 (DOE 8, Minnesota, USA) to screen which factor could influence the response. For hydrogel mixture, polyvinyl alcohol (PVA) and polyethylene glycol (PEG) were used. 10% PVA was mixed with 1% PEG (v/v) together with 1%...

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Main Authors:	Ab. Hadi, Hazrina, Abdul. Jalil, Mohd. Azri, Kasmuri, Abdul Razak
Format:	Conference or Workshop Item
Language:	English
Published:	2016
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/52532/ http://irep.iium.edu.my/52532/1/52532.pdf

id	iium-52532
recordtype	eprints
spelling	iium-525322017-06-14T06:13:26Z http://irep.iium.edu.my/52532/ Screening of the factors for hydrogel synthesis using design-of-experiment Ab. Hadi, Hazrina Abdul. Jalil, Mohd. Azri Kasmuri, Abdul Razak RS Pharmacy and materia medica The two level factorial design was chosen from design experiment software version 8 (DOE 8, Minnesota, USA) to screen which factor could influence the response. For hydrogel mixture, polyvinyl alcohol (PVA) and polyethylene glycol (PEG) were used. 10% PVA was mixed with 1% PEG (v/v) together with 1% protein-free agar solution (w/v), 2% dextran (w/v) and 6% honey (v/v). For the PVA aqueous solution, 100g PVA was dissolved in 600mL distilled water for an hour until the solution was clear. 10g of agar was dissolved in 330mL distilled water and heated for about one hour until clear. Then, 20g dextran was dissolved in the mixture. The solution was stirred continuously before 10mL of PEG was added to create agar solution. After that, the mixture of PVA aqueous solution and agar solution were submerged in the ultrasonic water bath at 37°C for an hour and half for degassing purpose. When the temperature reached 45°C, 60mL of honey was added. For a control hydrogel, similar mixture was prepared with the exclusion of honey. They were frozen at −20°C for 18 h and then thawed at 25°C for 6 h to complete a cycle (F-T cycle). They were group to first cycle until third cycle. From the formulation lists, three response (swelling ratio, water vapor transmission and gel fraction) were chosen to study the influence of the factors selected towards the formulations. For the swelling ratio (Y1) response, the most significant (p<0.05) factors were honey concentration (X1), agar concentration (X2) and number of cycles (X3). It indicated that these three factors could influence the swelling ratio response whereas the dextran concentration unable to influence the swelling ratio swelling ratio (Y1) response. A higher value of water vapor transmission rate could cause the wound to dry quickly and eventually produced scar. Whereas, a lower water vapor transmission rate may hinder the healing process due to the accumulating exudates. The acceptable value for water vapor transmission rate are 2000-25000 gm -2 day -1. For the gel fraction percentage, the higher percentage of gel fraction indicated a higher strength of the gel, but the flexibility was decreased. As a conclusion, from this screening tests, three factors which are honey concentration (X1), Agar concentration (X2), number of cycles (X3) showed significant values on the selected response. Based on these results, the optimization test will be conducted to generate the best formulation for hydrogel. 2016 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/52532/1/52532.pdf Ab. Hadi, Hazrina and Abdul. Jalil, Mohd. Azri and Kasmuri, Abdul Razak (2016) Screening of the factors for hydrogel synthesis using design-of-experiment. In: Pharma+BioAsia 2016 Conference, 28th-29th Sept. 2016, Kuala Lumpur. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Ab. Hadi, Hazrina Abdul. Jalil, Mohd. Azri Kasmuri, Abdul Razak Screening of the factors for hydrogel synthesis using design-of-experiment
description	The two level factorial design was chosen from design experiment software version 8 (DOE 8, Minnesota, USA) to screen which factor could influence the response. For hydrogel mixture, polyvinyl alcohol (PVA) and polyethylene glycol (PEG) were used. 10% PVA was mixed with 1% PEG (v/v) together with 1% protein-free agar solution (w/v), 2% dextran (w/v) and 6% honey (v/v). For the PVA aqueous solution, 100g PVA was dissolved in 600mL distilled water for an hour until the solution was clear. 10g of agar was dissolved in 330mL distilled water and heated for about one hour until clear. Then, 20g dextran was dissolved in the mixture. The solution was stirred continuously before 10mL of PEG was added to create agar solution. After that, the mixture of PVA aqueous solution and agar solution were submerged in the ultrasonic water bath at 37°C for an hour and half for degassing purpose. When the temperature reached 45°C, 60mL of honey was added. For a control hydrogel, similar mixture was prepared with the exclusion of honey. They were frozen at −20°C for 18 h and then thawed at 25°C for 6 h to complete a cycle (F-T cycle). They were group to first cycle until third cycle. From the formulation lists, three response (swelling ratio, water vapor transmission and gel fraction) were chosen to study the influence of the factors selected towards the formulations. For the swelling ratio (Y1) response, the most significant (p<0.05) factors were honey concentration (X1), agar concentration (X2) and number of cycles (X3). It indicated that these three factors could influence the swelling ratio response whereas the dextran concentration unable to influence the swelling ratio swelling ratio (Y1) response. A higher value of water vapor transmission rate could cause the wound to dry quickly and eventually produced scar. Whereas, a lower water vapor transmission rate may hinder the healing process due to the accumulating exudates. The acceptable value for water vapor transmission rate are 2000-25000 gm -2 day -1. For the gel fraction percentage, the higher percentage of gel fraction indicated a higher strength of the gel, but the flexibility was decreased. As a conclusion, from this screening tests, three factors which are honey concentration (X1), Agar concentration (X2), number of cycles (X3) showed significant values on the selected response. Based on these results, the optimization test will be conducted to generate the best formulation for hydrogel.
format	Conference or Workshop Item
author	Ab. Hadi, Hazrina Abdul. Jalil, Mohd. Azri Kasmuri, Abdul Razak
author_facet	Ab. Hadi, Hazrina Abdul. Jalil, Mohd. Azri Kasmuri, Abdul Razak
author_sort	Ab. Hadi, Hazrina
title	Screening of the factors for hydrogel synthesis using design-of-experiment
title_short	Screening of the factors for hydrogel synthesis using design-of-experiment
title_full	Screening of the factors for hydrogel synthesis using design-of-experiment
title_fullStr	Screening of the factors for hydrogel synthesis using design-of-experiment
title_full_unstemmed	Screening of the factors for hydrogel synthesis using design-of-experiment
title_sort	screening of the factors for hydrogel synthesis using design-of-experiment
publishDate	2016
url	http://irep.iium.edu.my/52532/ http://irep.iium.edu.my/52532/1/52532.pdf
first_indexed	2023-09-18T21:14:25Z
last_indexed	2023-09-18T21:14:25Z
_version_	1777411466847059968

Screening of the factors for hydrogel synthesis using design-of-experiment

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