Collagen-coated polylactic-glycolic acid (PLGA) seeded with neural-differentiated human mesenchymal stem cells as a potential nerve conduit.

Background. Autologous nerve grafts to bridge nerve gaps pose various drawbacks. Nerve tissue engineering to promote nerve regeneration using artificial neural conduits has emerged as a promising alternative. Objectives. To develop an artificial nerve conduit using collagen-coated polylactic-glyco...

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Main Authors:	Sulong, Ahmad Fadzli, Hassan, Nur Hidayah, Ng, Min Hwei, Lokanathan, Yogeswaran, Naicker, Amaramalar Selvi, Abdullah, Shalimar, Yusof, Mohd Reusmaazran, Htwe, Ohnmar, Idrus, Ruszymah, Mohamed Haflah, Nor Hazla
Format:	Article
Language:	English English English
Published:	Wroclaw Medical University 2014
Subjects:	RD701 Orthopedics
Online Access:	http://irep.iium.edu.my/52511/ http://irep.iium.edu.my/52511/ http://irep.iium.edu.my/52511/1/Collagen-Coated%20Polylactic-Glycolic%20Acid%20%28PLGA%29.PDF http://irep.iium.edu.my/52511/6/52511_Collagen-coated%20polylactic_WOS.pdf http://irep.iium.edu.my/52511/7/52511_Collagen-coated%20polylactic_SCOPUS.pdf

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spelling	iium-525112017-06-02T06:28:09Z http://irep.iium.edu.my/52511/ Collagen-coated polylactic-glycolic acid (PLGA) seeded with neural-differentiated human mesenchymal stem cells as a potential nerve conduit. Sulong, Ahmad Fadzli Hassan, Nur Hidayah Ng, Min Hwei Lokanathan, Yogeswaran Naicker, Amaramalar Selvi Abdullah, Shalimar Yusof, Mohd Reusmaazran Htwe, Ohnmar Idrus, Ruszymah Mohamed Haflah, Nor Hazla RD701 Orthopedics Background. Autologous nerve grafts to bridge nerve gaps pose various drawbacks. Nerve tissue engineering to promote nerve regeneration using artificial neural conduits has emerged as a promising alternative. Objectives. To develop an artificial nerve conduit using collagen-coated polylactic-glycolic acid (PLGA) and to analyse the survivability and propagating ability of the neuro-differentiated human mesenchymal stem cells in this conduit. Material and Methods. The PLGA conduit was constructed by dip-molding method and coated with collagen by immersing the conduit in collagen bath. The ultra structure of the conduits were examined before they were seeded with neural-differentiated human mesenchymal stem cells (nMSC) and implanted sub-muscularly on nude mice thighs. The non-collagen-coated PLGA conduit seeded with nMSC and non-seeded non-collagen-coated PLGA conduit were also implanted for comparison purposes. The survivability and propagation ability of nMSC was studied by histological and immunohistochemical analysis. Results. The collagen-coated conduits had a smooth inner wall and a highly porous outer wall. Conduits coated with collagen and seeded with nMSCs produced the most number of cells after 3 weeks. The best conduit based on the number of cells contained within it after 3 weeks was the collagen-coated PLGA conduit seeded with neuro-transdifferentiated cells. The collagen-coated PLGA conduit found to be suitable for attachment, survival and proliferation of the nMSC. Minimal cell infiltration was found in the implanted conduits where nearly all of the cells found in the cell seeded conduits are non-mouse origin and have neural cell markers, which exhibit the biocompatibility of the conduits. Conclusions. The collagen-coated PLGA conduit is biocompatible, non-cytotoxic and suitable for use as artificial nerve conduits Wroclaw Medical University 2014-05 Article PeerReviewed application/pdf en http://irep.iium.edu.my/52511/1/Collagen-Coated%20Polylactic-Glycolic%20Acid%20%28PLGA%29.PDF application/pdf en http://irep.iium.edu.my/52511/6/52511_Collagen-coated%20polylactic_WOS.pdf application/pdf en http://irep.iium.edu.my/52511/7/52511_Collagen-coated%20polylactic_SCOPUS.pdf Sulong, Ahmad Fadzli and Hassan, Nur Hidayah and Ng, Min Hwei and Lokanathan, Yogeswaran and Naicker, Amaramalar Selvi and Abdullah, Shalimar and Yusof, Mohd Reusmaazran and Htwe, Ohnmar and Idrus, Ruszymah and Mohamed Haflah, Nor Hazla (2014) Collagen-coated polylactic-glycolic acid (PLGA) seeded with neural-differentiated human mesenchymal stem cells as a potential nerve conduit. Advances in Clinical and Experimental Medicine, 23 (3). pp. 353-362. ISSN 1899–5276 E-ISSN 2451-2680 http://www.advances.umed.wroc.pl/pdf/2014/23/3/353.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	RD701 Orthopedics
spellingShingle	RD701 Orthopedics Sulong, Ahmad Fadzli Hassan, Nur Hidayah Ng, Min Hwei Lokanathan, Yogeswaran Naicker, Amaramalar Selvi Abdullah, Shalimar Yusof, Mohd Reusmaazran Htwe, Ohnmar Idrus, Ruszymah Mohamed Haflah, Nor Hazla Collagen-coated polylactic-glycolic acid (PLGA) seeded with neural-differentiated human mesenchymal stem cells as a potential nerve conduit.
description	Background. Autologous nerve grafts to bridge nerve gaps pose various drawbacks. Nerve tissue engineering to promote nerve regeneration using artificial neural conduits has emerged as a promising alternative. Objectives. To develop an artificial nerve conduit using collagen-coated polylactic-glycolic acid (PLGA) and to analyse the survivability and propagating ability of the neuro-differentiated human mesenchymal stem cells in this conduit. Material and Methods. The PLGA conduit was constructed by dip-molding method and coated with collagen by immersing the conduit in collagen bath. The ultra structure of the conduits were examined before they were seeded with neural-differentiated human mesenchymal stem cells (nMSC) and implanted sub-muscularly on nude mice thighs. The non-collagen-coated PLGA conduit seeded with nMSC and non-seeded non-collagen-coated PLGA conduit were also implanted for comparison purposes. The survivability and propagation ability of nMSC was studied by histological and immunohistochemical analysis. Results. The collagen-coated conduits had a smooth inner wall and a highly porous outer wall. Conduits coated with collagen and seeded with nMSCs produced the most number of cells after 3 weeks. The best conduit based on the number of cells contained within it after 3 weeks was the collagen-coated PLGA conduit seeded with neuro-transdifferentiated cells. The collagen-coated PLGA conduit found to be suitable for attachment, survival and proliferation of the nMSC. Minimal cell infiltration was found in the implanted conduits where nearly all of the cells found in the cell seeded conduits are non-mouse origin and have neural cell markers, which exhibit the biocompatibility of the conduits. Conclusions. The collagen-coated PLGA conduit is biocompatible, non-cytotoxic and suitable for use as artificial nerve conduits
format	Article
author	Sulong, Ahmad Fadzli Hassan, Nur Hidayah Ng, Min Hwei Lokanathan, Yogeswaran Naicker, Amaramalar Selvi Abdullah, Shalimar Yusof, Mohd Reusmaazran Htwe, Ohnmar Idrus, Ruszymah Mohamed Haflah, Nor Hazla
author_facet	Sulong, Ahmad Fadzli Hassan, Nur Hidayah Ng, Min Hwei Lokanathan, Yogeswaran Naicker, Amaramalar Selvi Abdullah, Shalimar Yusof, Mohd Reusmaazran Htwe, Ohnmar Idrus, Ruszymah Mohamed Haflah, Nor Hazla
author_sort	Sulong, Ahmad Fadzli
title	Collagen-coated polylactic-glycolic acid (PLGA) seeded with neural-differentiated human mesenchymal stem cells as a potential nerve conduit.
title_short	Collagen-coated polylactic-glycolic acid (PLGA) seeded with neural-differentiated human mesenchymal stem cells as a potential nerve conduit.
title_full	Collagen-coated polylactic-glycolic acid (PLGA) seeded with neural-differentiated human mesenchymal stem cells as a potential nerve conduit.
title_fullStr	Collagen-coated polylactic-glycolic acid (PLGA) seeded with neural-differentiated human mesenchymal stem cells as a potential nerve conduit.
title_full_unstemmed	Collagen-coated polylactic-glycolic acid (PLGA) seeded with neural-differentiated human mesenchymal stem cells as a potential nerve conduit.
title_sort	collagen-coated polylactic-glycolic acid (plga) seeded with neural-differentiated human mesenchymal stem cells as a potential nerve conduit.
publisher	Wroclaw Medical University
publishDate	2014
url	http://irep.iium.edu.my/52511/ http://irep.iium.edu.my/52511/ http://irep.iium.edu.my/52511/1/Collagen-Coated%20Polylactic-Glycolic%20Acid%20%28PLGA%29.PDF http://irep.iium.edu.my/52511/6/52511_Collagen-coated%20polylactic_WOS.pdf http://irep.iium.edu.my/52511/7/52511_Collagen-coated%20polylactic_SCOPUS.pdf
first_indexed	2023-09-18T21:14:23Z
last_indexed	2023-09-18T21:14:23Z
_version_	1777411465092792320

Collagen-coated polylactic-glycolic acid (PLGA) seeded with neural-differentiated human mesenchymal stem cells as a potential nerve conduit.

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