Genetic diversity of the orange-spotted grouper (Epinephelus coioides) in Terengganu Malaysia based on mitochondrial cytochrome b sequence data

Orange-spotted grouper (Epinephelus coioides) is known to be one of the economically high values marine. However, its spawning condition is still not well understood by the aqua culturist in Terengganu, Malaysia. Fish farmers depend on natural supply by col-lecting eggs of orange-spotted grouper fro...

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Main Authors:	Waludin, Shaidatul Nadia, Phang, Ing Chia, Mukai, Yukinori
Format:	Conference or Workshop Item
Language:	English
Published:	2016
Subjects:	SH Aquaculture. Fisheries. Angling
Online Access:	http://irep.iium.edu.my/52405/ http://irep.iium.edu.my/52405/ http://irep.iium.edu.my/52405/1/52405_Genetic%20Diversity%20of%20the%20Orange-spotted.pdf

id	iium-52405
recordtype	eprints
spelling	iium-524052016-10-27T03:02:00Z http://irep.iium.edu.my/52405/ Genetic diversity of the orange-spotted grouper (Epinephelus coioides) in Terengganu Malaysia based on mitochondrial cytochrome b sequence data Waludin, Shaidatul Nadia Phang, Ing Chia Mukai, Yukinori SH Aquaculture. Fisheries. Angling Orange-spotted grouper (Epinephelus coioides) is known to be one of the economically high values marine. However, its spawning condition is still not well understood by the aqua culturist in Terengganu, Malaysia. Fish farmers depend on natural supply by col-lecting eggs of orange-spotted grouper from the wild. A study was conducted to deter-mine the genetic diversity of farmed orange-spotted grouper population using partial Cytochrome b (Cyt-b) gene. A total of 20 farmed orange-spotted grouper were col-lected from a fish farm in Besut, Terengganu. A piece of dorsal fin area- (1 cm2) was individually clipped from the orange-spotted grouper and immediately preserved in 95% ethanol at ambient temperature upon sampling and kept on -80 ºC until DNA ex-traction was performed. DNA extraction was carried out using Qiagen Blood and Tis-sue kit. PCR amplification of partial Cyt-b was conducted using a universal Cyt-b pri-mer and amplicons of 491 bp was obtained. The amplicons were then purified, se-quenced and the genetic relationship among individuals was analyzed using MEGA6 software for their pairwise distance and phylogenetic tree. Phylogenetic analysis showed orange-spotted grouper is divided into two major groups. Genetic distance among individuals ranged between 0.000 – 0.014. These findings indicate that orange-spotted grouper has low genetic diversity and most of the individuals might be origi-nating from a small population. 2016-08 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/52405/1/52405_Genetic%20Diversity%20of%20the%20Orange-spotted.pdf Waludin, Shaidatul Nadia and Phang, Ing Chia and Mukai, Yukinori (2016) Genetic diversity of the orange-spotted grouper (Epinephelus coioides) in Terengganu Malaysia based on mitochondrial cytochrome b sequence data. In: 2nd International Conference on Oceanography and Sustainable Marine Production (ICOSMaP 2016), 23rd-25th Aug. 2016, Kuantan, Pahang. (Unpublished) http://www.iium.edu.my/icosmap/2016/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	SH Aquaculture. Fisheries. Angling
spellingShingle	SH Aquaculture. Fisheries. Angling Waludin, Shaidatul Nadia Phang, Ing Chia Mukai, Yukinori Genetic diversity of the orange-spotted grouper (Epinephelus coioides) in Terengganu Malaysia based on mitochondrial cytochrome b sequence data
description	Orange-spotted grouper (Epinephelus coioides) is known to be one of the economically high values marine. However, its spawning condition is still not well understood by the aqua culturist in Terengganu, Malaysia. Fish farmers depend on natural supply by col-lecting eggs of orange-spotted grouper from the wild. A study was conducted to deter-mine the genetic diversity of farmed orange-spotted grouper population using partial Cytochrome b (Cyt-b) gene. A total of 20 farmed orange-spotted grouper were col-lected from a fish farm in Besut, Terengganu. A piece of dorsal fin area- (1 cm2) was individually clipped from the orange-spotted grouper and immediately preserved in 95% ethanol at ambient temperature upon sampling and kept on -80 ºC until DNA ex-traction was performed. DNA extraction was carried out using Qiagen Blood and Tis-sue kit. PCR amplification of partial Cyt-b was conducted using a universal Cyt-b pri-mer and amplicons of 491 bp was obtained. The amplicons were then purified, se-quenced and the genetic relationship among individuals was analyzed using MEGA6 software for their pairwise distance and phylogenetic tree. Phylogenetic analysis showed orange-spotted grouper is divided into two major groups. Genetic distance among individuals ranged between 0.000 – 0.014. These findings indicate that orange-spotted grouper has low genetic diversity and most of the individuals might be origi-nating from a small population.
format	Conference or Workshop Item
author	Waludin, Shaidatul Nadia Phang, Ing Chia Mukai, Yukinori
author_facet	Waludin, Shaidatul Nadia Phang, Ing Chia Mukai, Yukinori
author_sort	Waludin, Shaidatul Nadia
title	Genetic diversity of the orange-spotted grouper (Epinephelus coioides) in Terengganu Malaysia based on mitochondrial cytochrome b sequence data
title_short	Genetic diversity of the orange-spotted grouper (Epinephelus coioides) in Terengganu Malaysia based on mitochondrial cytochrome b sequence data
title_full	Genetic diversity of the orange-spotted grouper (Epinephelus coioides) in Terengganu Malaysia based on mitochondrial cytochrome b sequence data
title_fullStr	Genetic diversity of the orange-spotted grouper (Epinephelus coioides) in Terengganu Malaysia based on mitochondrial cytochrome b sequence data
title_full_unstemmed	Genetic diversity of the orange-spotted grouper (Epinephelus coioides) in Terengganu Malaysia based on mitochondrial cytochrome b sequence data
title_sort	genetic diversity of the orange-spotted grouper (epinephelus coioides) in terengganu malaysia based on mitochondrial cytochrome b sequence data
publishDate	2016
url	http://irep.iium.edu.my/52405/ http://irep.iium.edu.my/52405/ http://irep.iium.edu.my/52405/1/52405_Genetic%20Diversity%20of%20the%20Orange-spotted.pdf
first_indexed	2023-09-18T21:14:15Z
last_indexed	2023-09-18T21:14:15Z
_version_	1777411456670629888

Genetic diversity of the orange-spotted grouper (Epinephelus coioides) in Terengganu Malaysia based on mitochondrial cytochrome b sequence data

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