Morphological and 18s rRNA sequencing of Euplotes sp.: a potential live feed for marine finfish larvae

Euplotes is a ciliated protozoan that is highly diverse in size and species and has poten-tial to be used as live feed for marine feed larvae. This study was conducted to identify the suitable Euplotes candidate through molecular and morphological methods. Euplotes sp. was characterized by its morph...

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Main Authors:	Waludin, Shaidatul Nadia, Afand, Muhammad Mu’izzuddin, Mohammad Noor, Normawaty, Phang, Ing Chia, Kadowaki, Syusaku, Mukai, Yukinori
Format:	Conference or Workshop Item
Language:	English
Published:	2016
Subjects:	SH Aquaculture. Fisheries. Angling
Online Access:	http://irep.iium.edu.my/52404/ http://irep.iium.edu.my/52404/ http://irep.iium.edu.my/52404/1/52404_MORPHOLOGICAL%20AND%2018S%20rRNA.pdf

id	iium-52404
recordtype	eprints
spelling	iium-524042016-10-27T02:29:23Z http://irep.iium.edu.my/52404/ Morphological and 18s rRNA sequencing of Euplotes sp.: a potential live feed for marine finfish larvae Waludin, Shaidatul Nadia Afand, Muhammad Mu’izzuddin Mohammad Noor, Normawaty Phang, Ing Chia Kadowaki, Syusaku Mukai, Yukinori SH Aquaculture. Fisheries. Angling Euplotes is a ciliated protozoan that is highly diverse in size and species and has poten-tial to be used as live feed for marine feed larvae. This study was conducted to identify the suitable Euplotes candidate through molecular and morphological methods. Euplotes sp. was characterized by its morphological and 18S rRNA gene sequence. The selected Euplotes sp. was identified morphologically based on its unique present of infracillia-ture, dorsal-ventrally flattened, dorsally ridged and oval in shaped. For molecular identi-fication, samples of Euplotes sp. were collected and starved overnight before pelleted through centrifugation to provide closely packed Euplotes species. The extraction and amplification of genomic DNA of Euplotes sp. were done using REDExtract-N-AmpTM Tissue PCR Kit (Sigma). The phylogenetic analyses of the SSU RNA gene was ana-lyzed with evolutionary distance calculated using Kimura-two-parameter and further analyzed by using Maximum Likelihood (ML) and Neighbor-joining (NJ) with Coris julis (accession number HM049942.1) as an out group. The result showed Euplotes sp. is 96% related to Euplotes encysticus (accession number LN864512.1) and Euplotes no-vemcarinatus (accession number HM140407.1). 2016-08 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/52404/1/52404_MORPHOLOGICAL%20AND%2018S%20rRNA.pdf Waludin, Shaidatul Nadia and Afand, Muhammad Mu’izzuddin and Mohammad Noor, Normawaty and Phang, Ing Chia and Kadowaki, Syusaku and Mukai, Yukinori (2016) Morphological and 18s rRNA sequencing of Euplotes sp.: a potential live feed for marine finfish larvae. In: 2nd International Conference on Oceanography and Sustainable Marine Production (ICOSMaP 2016), 23rd-25th Aug. 2016, Kuantan, Pahang. (Unpublished) http://www.iium.edu.my/icosmap/2016/wp-content/uploads/2016/08/ICOSMaP-2016.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	SH Aquaculture. Fisheries. Angling
spellingShingle	SH Aquaculture. Fisheries. Angling Waludin, Shaidatul Nadia Afand, Muhammad Mu’izzuddin Mohammad Noor, Normawaty Phang, Ing Chia Kadowaki, Syusaku Mukai, Yukinori Morphological and 18s rRNA sequencing of Euplotes sp.: a potential live feed for marine finfish larvae
description	Euplotes is a ciliated protozoan that is highly diverse in size and species and has poten-tial to be used as live feed for marine feed larvae. This study was conducted to identify the suitable Euplotes candidate through molecular and morphological methods. Euplotes sp. was characterized by its morphological and 18S rRNA gene sequence. The selected Euplotes sp. was identified morphologically based on its unique present of infracillia-ture, dorsal-ventrally flattened, dorsally ridged and oval in shaped. For molecular identi-fication, samples of Euplotes sp. were collected and starved overnight before pelleted through centrifugation to provide closely packed Euplotes species. The extraction and amplification of genomic DNA of Euplotes sp. were done using REDExtract-N-AmpTM Tissue PCR Kit (Sigma). The phylogenetic analyses of the SSU RNA gene was ana-lyzed with evolutionary distance calculated using Kimura-two-parameter and further analyzed by using Maximum Likelihood (ML) and Neighbor-joining (NJ) with Coris julis (accession number HM049942.1) as an out group. The result showed Euplotes sp. is 96% related to Euplotes encysticus (accession number LN864512.1) and Euplotes no-vemcarinatus (accession number HM140407.1).
format	Conference or Workshop Item
author	Waludin, Shaidatul Nadia Afand, Muhammad Mu’izzuddin Mohammad Noor, Normawaty Phang, Ing Chia Kadowaki, Syusaku Mukai, Yukinori
author_facet	Waludin, Shaidatul Nadia Afand, Muhammad Mu’izzuddin Mohammad Noor, Normawaty Phang, Ing Chia Kadowaki, Syusaku Mukai, Yukinori
author_sort	Waludin, Shaidatul Nadia
title	Morphological and 18s rRNA sequencing of Euplotes sp.: a potential live feed for marine finfish larvae
title_short	Morphological and 18s rRNA sequencing of Euplotes sp.: a potential live feed for marine finfish larvae
title_full	Morphological and 18s rRNA sequencing of Euplotes sp.: a potential live feed for marine finfish larvae
title_fullStr	Morphological and 18s rRNA sequencing of Euplotes sp.: a potential live feed for marine finfish larvae
title_full_unstemmed	Morphological and 18s rRNA sequencing of Euplotes sp.: a potential live feed for marine finfish larvae
title_sort	morphological and 18s rrna sequencing of euplotes sp.: a potential live feed for marine finfish larvae
publishDate	2016
url	http://irep.iium.edu.my/52404/ http://irep.iium.edu.my/52404/ http://irep.iium.edu.my/52404/1/52404_MORPHOLOGICAL%20AND%2018S%20rRNA.pdf
first_indexed	2023-09-18T21:14:15Z
last_indexed	2023-09-18T21:14:15Z
_version_	1777411456524877824

Morphological and 18s rRNA sequencing of Euplotes sp.: a potential live feed for marine finfish larvae

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