Investigation of anticancer (breast cancer) activity of Moringa oleifera leaf extracts

Moringa oleifera (M. oleifera) has been considered as food substance since ancient times and has also been used as a treatment for many diseases. Nowadays various therapeutic effects of M. oleifera such as antimicrobial, anticancer, anti-inflammatory, antidiabetic, and antioxidant effects have bee...

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Main Authors:	Mirghani, Mohamed Elwathig Saeed, Ahmad Raus, Raha, Hossain, Nazia
Format:	Conference or Workshop Item
Language:	English
Published:	Kulliyah of Engineering, International Islamic University Malaysia 2016
Subjects:	QK Botany RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Online Access:	http://irep.iium.edu.my/52205/ http://irep.iium.edu.my/52205/ http://irep.iium.edu.my/52205/1/52205.pdf

id	iium-52205
recordtype	eprints
spelling	iium-522052017-01-16T00:36:01Z http://irep.iium.edu.my/52205/ Investigation of anticancer (breast cancer) activity of Moringa oleifera leaf extracts Mirghani, Mohamed Elwathig Saeed Ahmad Raus, Raha Hossain, Nazia QK Botany RC0254 Neoplasms. Tumors. Oncology (including Cancer) Moringa oleifera (M. oleifera) has been considered as food substance since ancient times and has also been used as a treatment for many diseases. Nowadays various therapeutic effects of M. oleifera such as antimicrobial, anticancer, anti-inflammatory, antidiabetic, and antioxidant effects have been successfully investigated. In this study M. oleifera methanol extraction was done on breast cancer cell (MCF-7) to obtain anti-cancer activity by incorporation with microtitrate tetrazolium (MTT) assay and it inhibited MCF-7 cell line with 87.13% in average at wavelength A570nm. Cell-lines were seeded at 2x105 cells per ml in a 100µl of media (DMEM 90% and serum 10%) per well in 96 well plates. Moreover, an optimization study was done in terms of temperature and incubation time by sonication. The optimum condition was found to be temperature 50⁰C and incubation time 45 min with medium frequency at sonication and it obtained 88.39% cancer cell growth inhibition by this condition. Additionally, this extract was also implemented on normal cell line (VERO) and the extract inhibited only 12.89%. Thus it showed greater cytotoxicity for cancer cells than for normal cells, strongly suggested that it could potentially be an ideal anticancer therapeutic candidate specific to cancer cells. These results suggested that extraction strategy adopted in this work could lead to several industrial applications and the potential therapeutic implications of the soluble extract from M. oleifera leaves extract in the treatment of various types of cancers Kulliyah of Engineering, International Islamic University Malaysia 2016 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/52205/1/52205.pdf Mirghani, Mohamed Elwathig Saeed and Ahmad Raus, Raha and Hossain, Nazia (2016) Investigation of anticancer (breast cancer) activity of Moringa oleifera leaf extracts. In: 4th International Conference on Biotechnology Engineering 2016 (ICBioE 2016), 25th-27th July 2016, Kuala Lumpur. http://www.iium.edu.my/icbioe/2016/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QK Botany RC0254 Neoplasms. Tumors. Oncology (including Cancer)
spellingShingle	QK Botany RC0254 Neoplasms. Tumors. Oncology (including Cancer) Mirghani, Mohamed Elwathig Saeed Ahmad Raus, Raha Hossain, Nazia Investigation of anticancer (breast cancer) activity of Moringa oleifera leaf extracts
description	Moringa oleifera (M. oleifera) has been considered as food substance since ancient times and has also been used as a treatment for many diseases. Nowadays various therapeutic effects of M. oleifera such as antimicrobial, anticancer, anti-inflammatory, antidiabetic, and antioxidant effects have been successfully investigated. In this study M. oleifera methanol extraction was done on breast cancer cell (MCF-7) to obtain anti-cancer activity by incorporation with microtitrate tetrazolium (MTT) assay and it inhibited MCF-7 cell line with 87.13% in average at wavelength A570nm. Cell-lines were seeded at 2x105 cells per ml in a 100µl of media (DMEM 90% and serum 10%) per well in 96 well plates. Moreover, an optimization study was done in terms of temperature and incubation time by sonication. The optimum condition was found to be temperature 50⁰C and incubation time 45 min with medium frequency at sonication and it obtained 88.39% cancer cell growth inhibition by this condition. Additionally, this extract was also implemented on normal cell line (VERO) and the extract inhibited only 12.89%. Thus it showed greater cytotoxicity for cancer cells than for normal cells, strongly suggested that it could potentially be an ideal anticancer therapeutic candidate specific to cancer cells. These results suggested that extraction strategy adopted in this work could lead to several industrial applications and the potential therapeutic implications of the soluble extract from M. oleifera leaves extract in the treatment of various types of cancers
format	Conference or Workshop Item
author	Mirghani, Mohamed Elwathig Saeed Ahmad Raus, Raha Hossain, Nazia
author_facet	Mirghani, Mohamed Elwathig Saeed Ahmad Raus, Raha Hossain, Nazia
author_sort	Mirghani, Mohamed Elwathig Saeed
title	Investigation of anticancer (breast cancer) activity of Moringa oleifera leaf extracts
title_short	Investigation of anticancer (breast cancer) activity of Moringa oleifera leaf extracts
title_full	Investigation of anticancer (breast cancer) activity of Moringa oleifera leaf extracts
title_fullStr	Investigation of anticancer (breast cancer) activity of Moringa oleifera leaf extracts
title_full_unstemmed	Investigation of anticancer (breast cancer) activity of Moringa oleifera leaf extracts
title_sort	investigation of anticancer (breast cancer) activity of moringa oleifera leaf extracts
publisher	Kulliyah of Engineering, International Islamic University Malaysia
publishDate	2016
url	http://irep.iium.edu.my/52205/ http://irep.iium.edu.my/52205/ http://irep.iium.edu.my/52205/1/52205.pdf
first_indexed	2023-09-18T21:14:00Z
last_indexed	2023-09-18T21:14:00Z
_version_	1777411440525705216

Investigation of anticancer (breast cancer) activity of Moringa oleifera leaf extracts

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