Tualang honey improves non alcoholic steatohepatitis animal model
Introduction: Non-alcoholic Steatohepatitis (NASH) is an emerging chronic liver disease with limited therapy available. Studies utilizing animal models induced with cholesterol diet ranging from 1-2% are hampered by inconsistent yield of NASH features. Therefore, we aimed to establish a NASH animal...
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| Language: | English |
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2016
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| Online Access: | http://irep.iium.edu.my/51857/ http://irep.iium.edu.my/51857/42/51857.pdf |
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iium-51857 |
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eprints |
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iium-518572016-09-09T02:25:38Z http://irep.iium.edu.my/51857/ Tualang honey improves non alcoholic steatohepatitis animal model Abdul Ghani, Azril Shahreez Abdullah, Nor Zamzila Muhammad, Siti Aesah @ Naznin Abdul Rahim, Roslina QP Physiology R Medicine (General) RA Public aspects of medicine RB Pathology RC Internal medicine RD Surgery Introduction: Non-alcoholic Steatohepatitis (NASH) is an emerging chronic liver disease with limited therapy available. Studies utilizing animal models induced with cholesterol diet ranging from 1-2% are hampered by inconsistent yield of NASH features. Therefore, we aimed to establish a NASH animal model utilizing 12% cholesterol diet (CD) and to investigate the effects of Tualang honey (TH) known for its anti-inflammatory and anti-oxidative properties in this model. Methods: Twenty-four Sprague-Dawley rats were divided into 2 groups (12% CD and standard diet) and were fed for 6 weeks. Following the establishment of NASH, the rats in the 12% CD group were subsequently divided into 3 groups. The first group was continued with only 12% CD. In the other 2 groups in addition to the 12% CD they were given TH treatment at different concentrations (1.2 and 2.4 g/kg/day) for 4 weeks. Blood biochemical analysis and histological assessment of liver were subsequently performed. Results: The liver histological sections of the rats fed with 12% CD showed macrovesicular steatosis, ballooning degeneration with lobular and portal inflammation. They also had increased serum alanine aminotransferase (ALT), total cholesterol (TC), low density lipoprotein cholesterol (LDL-C), fasting insulin, HOMA-IR and reduced high density lipoprotein cholesterol (HDL-C). Meanwhile, the TH treatment groups exhibited significant improvement in both the NASH grading and activity scores. The ALT, LDL-C, TC, triglyceride (TG), fasting insulin and HOMA-IR levels were reduced significantly. Conclusions: The 12% CD was able to induce NASH in the animal model. Tualang honey improved insulin sensitivity, dyslipidaemia, steatohepatitis. 2016-08 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/51857/42/51857.pdf Abdul Ghani, Azril Shahreez and Abdullah, Nor Zamzila and Muhammad, Siti Aesah @ Naznin and Abdul Rahim, Roslina (2016) Tualang honey improves non alcoholic steatohepatitis animal model. In: Medical Research Symposium & Pacific Partnership in conjunction with Kuantan Research Day 2016, 3rd-5th August 2016, Kuantan, Pahang, Malaysia. (Unpublished) |
| repository_type |
Digital Repository |
| institution_category |
Local University |
| institution |
International Islamic University Malaysia |
| building |
IIUM Repository |
| collection |
Online Access |
| language |
English |
| topic |
QP Physiology R Medicine (General) RA Public aspects of medicine RB Pathology RC Internal medicine RD Surgery |
| spellingShingle |
QP Physiology R Medicine (General) RA Public aspects of medicine RB Pathology RC Internal medicine RD Surgery Abdul Ghani, Azril Shahreez Abdullah, Nor Zamzila Muhammad, Siti Aesah @ Naznin Abdul Rahim, Roslina Tualang honey improves non alcoholic steatohepatitis animal model |
| description |
Introduction: Non-alcoholic Steatohepatitis (NASH) is an emerging chronic liver disease with limited therapy available. Studies utilizing animal models induced with cholesterol diet ranging from 1-2% are hampered by inconsistent yield of NASH features. Therefore, we aimed to establish a NASH animal model utilizing 12% cholesterol diet (CD) and to investigate the effects of Tualang honey (TH) known for its anti-inflammatory and anti-oxidative properties in this model. Methods: Twenty-four Sprague-Dawley rats were divided into 2 groups (12% CD and standard diet) and were fed for 6 weeks. Following the establishment of NASH, the rats in the 12% CD group were subsequently divided into 3 groups. The first group was continued with only 12% CD. In the other 2 groups in addition to the 12% CD they were given TH treatment at different concentrations (1.2 and 2.4 g/kg/day) for 4 weeks. Blood biochemical analysis and histological assessment of liver were subsequently performed. Results: The liver histological sections of the rats fed with 12% CD showed macrovesicular steatosis, ballooning degeneration with lobular and portal inflammation. They also had increased serum alanine aminotransferase (ALT), total cholesterol (TC), low density lipoprotein cholesterol (LDL-C), fasting insulin, HOMA-IR and reduced high density lipoprotein cholesterol (HDL-C). Meanwhile, the TH treatment groups exhibited significant improvement in both the NASH grading and activity scores. The ALT, LDL-C, TC, triglyceride (TG), fasting insulin and HOMA-IR levels were reduced significantly. Conclusions: The 12% CD was able to induce NASH in the animal model. Tualang honey improved insulin sensitivity, dyslipidaemia, steatohepatitis. |
| format |
Conference or Workshop Item |
| author |
Abdul Ghani, Azril Shahreez Abdullah, Nor Zamzila Muhammad, Siti Aesah @ Naznin Abdul Rahim, Roslina |
| author_facet |
Abdul Ghani, Azril Shahreez Abdullah, Nor Zamzila Muhammad, Siti Aesah @ Naznin Abdul Rahim, Roslina |
| author_sort |
Abdul Ghani, Azril Shahreez |
| title |
Tualang honey improves non alcoholic steatohepatitis animal model |
| title_short |
Tualang honey improves non alcoholic steatohepatitis animal model |
| title_full |
Tualang honey improves non alcoholic steatohepatitis animal model |
| title_fullStr |
Tualang honey improves non alcoholic steatohepatitis animal model |
| title_full_unstemmed |
Tualang honey improves non alcoholic steatohepatitis animal model |
| title_sort |
tualang honey improves non alcoholic steatohepatitis animal model |
| publishDate |
2016 |
| url |
http://irep.iium.edu.my/51857/ http://irep.iium.edu.my/51857/42/51857.pdf |
| first_indexed |
2023-09-18T21:13:31Z |
| last_indexed |
2023-09-18T21:13:31Z |
| _version_ |
1777411410431574016 |