Lipid modulation activity of garcinia atroviridis extract on 3T3-L1 cell line

Obesity is a global public health problem and it is a risk factor for many chronic diseases such as diabetes mellitus, cardiovascular illness and cancer. Malaysia has been rated as the highest among Asian countries for obesity. The fruit rind of Garcinia atroviridis, also known as ‘Asam Gelugor’, is...

Full description

Bibliographic Details
Main Authors:	Hamidon, Hanisuhana, Bakhtiar, M. Taher, Darnis, Deny Susanti
Format:	Conference or Workshop Item
Language:	English English
Published:	2016
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/51812/ http://irep.iium.edu.my/51812/1/51812%20Lipid%20Modulation%20Activity.pdf http://irep.iium.edu.my/51812/2/51812.pdf

id	iium-51812
recordtype	eprints
spelling	iium-518122018-02-25T09:33:35Z http://irep.iium.edu.my/51812/ Lipid modulation activity of garcinia atroviridis extract on 3T3-L1 cell line Hamidon, Hanisuhana Bakhtiar, M. Taher Darnis, Deny Susanti RS Pharmacy and materia medica Obesity is a global public health problem and it is a risk factor for many chronic diseases such as diabetes mellitus, cardiovascular illness and cancer. Malaysia has been rated as the highest among Asian countries for obesity. The fruit rind of Garcinia atroviridis, also known as ‘Asam Gelugor’, is one of sources for hydroxycitric acid (HCA). HCA is a phytochemical that have potential as weight management agent and it recently gained popularity among public. This present study was done to investigate the lipid modulation ability of G. atroviridis fruit extract in vitro using 3T3-L1 cell line. Fresh fruit of G. atroviridis was macerated using ethanol, rota-vapourised and freeze-dried yielding powdered extract. Then, cell viability assay was conducted using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide salt (MTT). Three concentrations were chosen to be used in adipocyte differentiation, adipolysis and leptin assay. Differentiated adipocytes were analysed qualitatively and quantitatively using Oil Red O stain. Adipolysis and leptin assay was done using adipolysis and leptin ELISA kits respectively. Fruit extract was found to be not toxic on cell line used. At 10, 45 and 60 µg/mL concentrations tested, the extract inhibit adipocytes differentiation significantly as compared to HCA salt. Leptin released was also decreased at increasing concentration used. Meanwhile, the same concentrations tested was found to be promoting adipocyte breakdown as measured by glycerol concentration. Based on our present findings, the G. atroviridis fruit can be proposed as one of weight management agents due to its lipid modulation properties. 2016-08 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/51812/1/51812%20Lipid%20Modulation%20Activity.pdf application/pdf en http://irep.iium.edu.my/51812/2/51812.pdf Hamidon, Hanisuhana and Bakhtiar, M. Taher and Darnis, Deny Susanti (2016) Lipid modulation activity of garcinia atroviridis extract on 3T3-L1 cell line. In: 30th Scientific Meeting of Malaysian Society of Pharmacology and Physiology (MSPP) 2016, 15th-16th Aug. 2016, Putrajaya.
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Hamidon, Hanisuhana Bakhtiar, M. Taher Darnis, Deny Susanti Lipid modulation activity of garcinia atroviridis extract on 3T3-L1 cell line
description	Obesity is a global public health problem and it is a risk factor for many chronic diseases such as diabetes mellitus, cardiovascular illness and cancer. Malaysia has been rated as the highest among Asian countries for obesity. The fruit rind of Garcinia atroviridis, also known as ‘Asam Gelugor’, is one of sources for hydroxycitric acid (HCA). HCA is a phytochemical that have potential as weight management agent and it recently gained popularity among public. This present study was done to investigate the lipid modulation ability of G. atroviridis fruit extract in vitro using 3T3-L1 cell line. Fresh fruit of G. atroviridis was macerated using ethanol, rota-vapourised and freeze-dried yielding powdered extract. Then, cell viability assay was conducted using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide salt (MTT). Three concentrations were chosen to be used in adipocyte differentiation, adipolysis and leptin assay. Differentiated adipocytes were analysed qualitatively and quantitatively using Oil Red O stain. Adipolysis and leptin assay was done using adipolysis and leptin ELISA kits respectively. Fruit extract was found to be not toxic on cell line used. At 10, 45 and 60 µg/mL concentrations tested, the extract inhibit adipocytes differentiation significantly as compared to HCA salt. Leptin released was also decreased at increasing concentration used. Meanwhile, the same concentrations tested was found to be promoting adipocyte breakdown as measured by glycerol concentration. Based on our present findings, the G. atroviridis fruit can be proposed as one of weight management agents due to its lipid modulation properties.
format	Conference or Workshop Item
author	Hamidon, Hanisuhana Bakhtiar, M. Taher Darnis, Deny Susanti
author_facet	Hamidon, Hanisuhana Bakhtiar, M. Taher Darnis, Deny Susanti
author_sort	Hamidon, Hanisuhana
title	Lipid modulation activity of garcinia atroviridis extract on 3T3-L1 cell line
title_short	Lipid modulation activity of garcinia atroviridis extract on 3T3-L1 cell line
title_full	Lipid modulation activity of garcinia atroviridis extract on 3T3-L1 cell line
title_fullStr	Lipid modulation activity of garcinia atroviridis extract on 3T3-L1 cell line
title_full_unstemmed	Lipid modulation activity of garcinia atroviridis extract on 3T3-L1 cell line
title_sort	lipid modulation activity of garcinia atroviridis extract on 3t3-l1 cell line
publishDate	2016
url	http://irep.iium.edu.my/51812/ http://irep.iium.edu.my/51812/1/51812%20Lipid%20Modulation%20Activity.pdf http://irep.iium.edu.my/51812/2/51812.pdf
first_indexed	2023-09-18T21:13:27Z
last_indexed	2023-09-18T21:13:27Z
_version_	1777411406191132672

Lipid modulation activity of garcinia atroviridis extract on 3T3-L1 cell line

Similar Items