Lipid modulation activity of garcinia atroviridis extract on 3T3-L1 cell line
Obesity is a global public health problem and it is a risk factor for many chronic diseases such as diabetes mellitus, cardiovascular illness and cancer. Malaysia has been rated as the highest among Asian countries for obesity. The fruit rind of Garcinia atroviridis, also known as ‘Asam Gelugor’, is...
| Main Authors: | , , |
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| Format: | Conference or Workshop Item |
| Language: | English English |
| Published: |
2016
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/51812/ http://irep.iium.edu.my/51812/1/51812%20Lipid%20Modulation%20Activity.pdf http://irep.iium.edu.my/51812/2/51812.pdf |
| Summary: | Obesity is a global public health problem and it is a risk factor for many chronic diseases such as diabetes mellitus, cardiovascular illness and cancer. Malaysia has been rated as the highest among Asian countries for obesity. The fruit rind of Garcinia atroviridis, also known as ‘Asam Gelugor’, is one of sources for hydroxycitric acid (HCA). HCA is a phytochemical that have potential as weight management agent and it recently gained popularity among public. This present study was done to investigate the lipid modulation ability of G. atroviridis fruit extract in vitro using 3T3-L1 cell line. Fresh fruit of G. atroviridis was macerated using ethanol, rota-vapourised and freeze-dried yielding powdered extract. Then, cell viability assay was conducted using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide salt (MTT). Three concentrations were chosen to be used in adipocyte differentiation, adipolysis and leptin assay. Differentiated adipocytes were analysed qualitatively and quantitatively using Oil Red O stain. Adipolysis and leptin assay was done using adipolysis and leptin ELISA kits respectively. Fruit extract was found to be not toxic on cell line used. At 10, 45 and 60 µg/mL concentrations tested, the extract inhibit adipocytes differentiation significantly as compared to HCA salt. Leptin released was also decreased at increasing concentration used. Meanwhile, the same concentrations tested was found to be promoting adipocyte breakdown as measured by glycerol concentration. Based on our present findings, the G. atroviridis fruit can be proposed as one of weight management agents due to its lipid modulation properties. |
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