Effect of media compositions on bioprotein production by mixed culture of Phanerochaete chrysosporium and Candida utilis using palm kernel cake and seaweed as substrates

In aqua-cultural production, feeding represent over 50% of the production cost of which protein constitute the most expensive dietary source. Bioprotein is a promising replacement for the commercial fish feed supplement. In this study, the enrichment of the bioprotein content after solid state fer...

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Main Authors:	Ilias, Nurul Najihah, Jamal, Parveen, Sulaiman, Sarina, Jaswir, Irwandi, Azmi, Azlin Suhaida, Zainudin , Zaki
Format:	Conference or Workshop Item
Language:	English
Published:	Kulliyah of Engineering, International Islamic University Malaysia 2016
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/51761/ http://irep.iium.edu.my/51761/ http://irep.iium.edu.my/51761/1/51761.pdf

id	iium-51761
recordtype	eprints
spelling	iium-517612017-01-17T01:32:29Z http://irep.iium.edu.my/51761/ Effect of media compositions on bioprotein production by mixed culture of Phanerochaete chrysosporium and Candida utilis using palm kernel cake and seaweed as substrates Ilias, Nurul Najihah Jamal, Parveen Sulaiman, Sarina Jaswir, Irwandi Azmi, Azlin Suhaida Zainudin , Zaki TP248.13 Biotechnology In aqua-cultural production, feeding represent over 50% of the production cost of which protein constitute the most expensive dietary source. Bioprotein is a promising replacement for the commercial fish feed supplement. In this study, the enrichment of the bioprotein content after solid state fermentation using palm kernel cake and seaweed by the white rot fungus; Phanerochaete chrysoporium and yeast; Candida utilis was investigated. The growth media components were selected from 11 types of media using Plackett-Burman design (PBD) and were optimized by one-factor-at-a-time (OFAT) method with bioprotein concentration (mg/g) as the response. From the screening result using PBD, three media components; K2HPO4, CuSO4.5H2O and MnSO4.H2O were selected for further optimization using OFAT method due to their positive contributions to the response. The final results showed that 5.0 g/L K2HPO4, 3.0 g/L CuSO4.5H2O and 0.1 g/L MnSO4.H2O were found to be the optimum media constituents with 9.0 g/L, MgSO4.7H2O, 0.1 g/L, CaCl2.H2O, 3.0 g/L FeSO4.7H2O and 3.0 g/L peptone as fixed compositions. At this optimum concentration, the protein increment of 11% was observed as compared to the results obtained by using PBD. It was found that mixed cultures can improve the protein concentrations which can be used as fish feed. Kulliyah of Engineering, International Islamic University Malaysia 2016 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/51761/1/51761.pdf Ilias, Nurul Najihah and Jamal, Parveen and Sulaiman, Sarina and Jaswir, Irwandi and Azmi, Azlin Suhaida and Zainudin , Zaki (2016) Effect of media compositions on bioprotein production by mixed culture of Phanerochaete chrysosporium and Candida utilis using palm kernel cake and seaweed as substrates. In: 4th International Conference on Biotechnology Engineering 2016 (ICBioE 2016), 25th-27th July 2016, Kuala Lumpur. http://www.iium.edu.my/icbioe/2016/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Ilias, Nurul Najihah Jamal, Parveen Sulaiman, Sarina Jaswir, Irwandi Azmi, Azlin Suhaida Zainudin , Zaki Effect of media compositions on bioprotein production by mixed culture of Phanerochaete chrysosporium and Candida utilis using palm kernel cake and seaweed as substrates
description	In aqua-cultural production, feeding represent over 50% of the production cost of which protein constitute the most expensive dietary source. Bioprotein is a promising replacement for the commercial fish feed supplement. In this study, the enrichment of the bioprotein content after solid state fermentation using palm kernel cake and seaweed by the white rot fungus; Phanerochaete chrysoporium and yeast; Candida utilis was investigated. The growth media components were selected from 11 types of media using Plackett-Burman design (PBD) and were optimized by one-factor-at-a-time (OFAT) method with bioprotein concentration (mg/g) as the response. From the screening result using PBD, three media components; K2HPO4, CuSO4.5H2O and MnSO4.H2O were selected for further optimization using OFAT method due to their positive contributions to the response. The final results showed that 5.0 g/L K2HPO4, 3.0 g/L CuSO4.5H2O and 0.1 g/L MnSO4.H2O were found to be the optimum media constituents with 9.0 g/L, MgSO4.7H2O, 0.1 g/L, CaCl2.H2O, 3.0 g/L FeSO4.7H2O and 3.0 g/L peptone as fixed compositions. At this optimum concentration, the protein increment of 11% was observed as compared to the results obtained by using PBD. It was found that mixed cultures can improve the protein concentrations which can be used as fish feed.
format	Conference or Workshop Item
author	Ilias, Nurul Najihah Jamal, Parveen Sulaiman, Sarina Jaswir, Irwandi Azmi, Azlin Suhaida Zainudin , Zaki
author_facet	Ilias, Nurul Najihah Jamal, Parveen Sulaiman, Sarina Jaswir, Irwandi Azmi, Azlin Suhaida Zainudin , Zaki
author_sort	Ilias, Nurul Najihah
title	Effect of media compositions on bioprotein production by mixed culture of Phanerochaete chrysosporium and Candida utilis using palm kernel cake and seaweed as substrates
title_short	Effect of media compositions on bioprotein production by mixed culture of Phanerochaete chrysosporium and Candida utilis using palm kernel cake and seaweed as substrates
title_full	Effect of media compositions on bioprotein production by mixed culture of Phanerochaete chrysosporium and Candida utilis using palm kernel cake and seaweed as substrates
title_fullStr	Effect of media compositions on bioprotein production by mixed culture of Phanerochaete chrysosporium and Candida utilis using palm kernel cake and seaweed as substrates
title_full_unstemmed	Effect of media compositions on bioprotein production by mixed culture of Phanerochaete chrysosporium and Candida utilis using palm kernel cake and seaweed as substrates
title_sort	effect of media compositions on bioprotein production by mixed culture of phanerochaete chrysosporium and candida utilis using palm kernel cake and seaweed as substrates
publisher	Kulliyah of Engineering, International Islamic University Malaysia
publishDate	2016
url	http://irep.iium.edu.my/51761/ http://irep.iium.edu.my/51761/ http://irep.iium.edu.my/51761/1/51761.pdf
first_indexed	2023-09-18T21:13:22Z
last_indexed	2023-09-18T21:13:22Z
_version_	1777411400757411840

Effect of media compositions on bioprotein production by mixed culture of Phanerochaete chrysosporium and Candida utilis using palm kernel cake and seaweed as substrates

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