Kinetic performance of cross-linked enzyme aggregates (clea)-amylase from Zophobas morio
Many studies have been done on various species of insects to investigate their potential use in industries. This is because insects have high protein content which could be further manipulated. Due to its eating habit, Zophobas morio larvae, also known as supermeal worm has been shown to have high...
| Main Authors: | , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
Kulliyah of Engineering, International Islamic University Malaysia
2016
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/51744/ http://irep.iium.edu.my/51744/ http://irep.iium.edu.my/51744/1/51744.pdf |
| Summary: | Many studies have been done on various species of insects to investigate their potential use in industries. This
is because insects have high protein content which could be further manipulated. Due to its eating habit,
Zophobas morio larvae, also known as supermeal worm has been shown to have high amylase activity. In this
study, amylase from supermeal worm has been immobilized via Cross-Linked Enzyme Aggregates (CLEA)
technique and its kinetic performance, evaluated. CLEA is one of the best immobilization technology in
respect to enzyme stability and reusability. Kinetic performance of both free and CLEA-amylase were
evaluated based on the Michaelis-Menten model. Results obtained based Hanes-Woolf, Lineweaver-Burk,
Eadie-Hofstee and Hyperbolic Regression plots, showed that the kinetic parameters, Vmax and KM, changed
upon immobilization. For CLEA-amylase, Hanes-Woolf plot had showed the best fitted model based on R2
with Vmax= 1.068 mM/min and KM= 0.182 mM. Lineweaver-Burk plot was used to obtain the kinetic
parameters for free amylase, with Vmax and KM of 17.230 mM/min and 2.470 mM, respectively. The result
from this study might open the new path in discovering the potential of using insects in industrial
applications, for example, in detergent industry |
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