Effect of some parameters on the extraction process of antifungal peptides from Supermeal worm, Zophobas morio (Fabricius)
Acidified isopropanol extract of whole body larvae of Zophobas morio (Fabricius), which contains peptides, has been shown to exhibit an inhibitory effect towards fungal growth. The larvae, commonly known as supermeal worm are cheap and easily maintained. To make the extraction even more cost effec...
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
Kulliyah of Engineering, International Islamic University Malaysia
2016
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| Online Access: | http://irep.iium.edu.my/51733/ http://irep.iium.edu.my/51733/ http://irep.iium.edu.my/51733/1/51733.pdf |
| Summary: | Acidified isopropanol extract of whole body larvae of Zophobas morio (Fabricius), which contains peptides,
has been shown to exhibit an inhibitory effect towards fungal growth. The larvae, commonly known as
supermeal worm are cheap and easily maintained. To make the extraction even more cost effective, it is
pertinent to maximize the extraction yield and to optimize the extraction process. The aim of this study is to
use the One-Factor-At-a-Time (OFAT) strategy to determine the maximum values of the process parameters
for the extraction of antifungal peptides, where these values can later be used in the design to optimize the
extraction process. Based on importance, three parameters were selected, namely, initial homogenization
temperature, homogenization time and solid (g) to solvent (ml) ratio. Maximum inhibition to fungal growth
was found when the extraction was carried out as follows; using initial homogenization temperature of 4°C,
homogenization time of 5 minutes and a solid (g) to solvent (ml) ratio of 3.5:1. The peptide extract displayed
different degree of antifungal effect towards four selected fungi, Aspergillus niger, Microsporum canis,
Candida albicans and Blastomyces dermatitidsis.
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