An approach to optimize process parameter for peptides extraction from Zophobas morio (Fabricius) using antifungal activity as the response
Antifungal peptides have been successfully extracted from whole body larvae of Zophobas morio (Fabricius) by using acidified isopropanol. To ensure that the extraction is cost effective for maximum yield, Response Surface Methodology (RSM) using a Central Composite Design (CCD) strategy was adopte...
| Main Authors: | , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
Kulliyah of Engineering, International Islamic University Malaysia
2016
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/51732/ http://irep.iium.edu.my/51732/ http://irep.iium.edu.my/51732/1/51732.pdf |
| Summary: | Antifungal peptides have been successfully extracted from whole body larvae of Zophobas morio (Fabricius)
by using acidified isopropanol. To ensure that the extraction is cost effective for maximum yield, Response
Surface Methodology (RSM) using a Central Composite Design (CCD) strategy was adopted to optimize the
extraction process parameters. The effect of independent parameters, namely, the homogenization
temperature (°C), homogenization time (min) and solid (g) to solvent (ml) ratio of the extraction process on
the fungal growth was studied. The extracted samples showed varying degree of antifungal activity against
Aspergillus niger, the selected fungal strain, by using poisoned agar assay technique. Response surface
investigation showed that the optimum values of the extraction parameters for antifungal peptides were 5
minutes homogenization time, 4°C homogenization temperature and 3.5:1 solid to solvent ratio. This study
reports the development of an extraction process that allows careful recovery of antifungal peptides from
insect larvae. In the validation of the experimental model, the error between the actual value and the
predicted value was determined to be 3.54%. |
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