Molecular study of hepatitis C viral RNA extracted from local isolates in Pahang, Malaysia: genotyping, subtyping and base sequencing

Hepatitis C virus infection affects approximately 170 million individuals constituting about 3% of the world's population. Most of those infected face the risk of developing liver cirrhosis and/or liver cancer. In Malaysia, hepatitis C prevalence is 1.6% and is still the foremost infection amo...

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Main Authors:	Hamzah, Hairul Aini, Mustafa Mahmoud, Mohammed Imad Al-Deen, Abdul Majid, Mohammed Saad, Hasmoni, Mohamed Hadzri, Abdullah, Nasuruddin
Format:	Conference or Workshop Item
Language:	English
Published:	2010
Subjects:	QR355 Virology
Online Access:	http://irep.iium.edu.my/5172/ http://irep.iium.edu.my/5172/1/Poster_IRIIE_2010.pdf

id	iium-5172
recordtype	eprints
spelling	iium-51722017-06-15T01:04:43Z http://irep.iium.edu.my/5172/ Molecular study of hepatitis C viral RNA extracted from local isolates in Pahang, Malaysia: genotyping, subtyping and base sequencing Hamzah, Hairul Aini Mustafa Mahmoud, Mohammed Imad Al-Deen Abdul Majid, Mohammed Saad Hasmoni, Mohamed Hadzri Abdullah, Nasuruddin QR355 Virology Hepatitis C virus infection affects approximately 170 million individuals constituting about 3% of the world's population. Most of those infected face the risk of developing liver cirrhosis and/or liver cancer. In Malaysia, hepatitis C prevalence is 1.6% and is still the foremost infection among multiple blood transfusion groups. The current mainstay treatment of HCV is pegylated alpha-interferon in combination with ribavirin, incurring considerable expense on local health services. In fact, less than 50% of treated patients respond favorably to the given therapy. Understanding the characteristics of the RNA genome of the local HCV genotypes can serve as foundation for future development of rapid diagnostic techniques. In addition, it has the potential for helping in designing small interfering RNA (siRNA) to be utilized in studies related to specific silencing of vital viral genes. However, despite the plethora of global HCV studies, there is relative scarcity HCV research in Malaysia. In this present study, HCV isolates from infected haemodialysis patients were studied, focusing on the characterization of their genomes, by genotyping and base-sequencing. The nucleotide sequence of the conserved 5’UTR region of HCV genome revealed several sequence patterns across the 4 main HCV genotypes available in the study panel. Phylogenetic analysis of the NS5B region showed a predominance of HCV genotype 3a. The revealed sequence patterns have the potential for designing probes that could differentiate the predominant HCV genotype 3 from other genotypes. Analysis of the secondary structure of genotype 3a showed conserved loop structures that could be targeted by small interfering RNA molecules. In conclusion, molecular studies of local HCV strains provide a new dimension for the improvement of current HCV detection and genotyping methods, aid in better understanding of the molecular epidemiology of the virus infection and may form the basis for future in-vitro studies on viral molecular pathogenetic mechanism 2010-01-26 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/5172/1/Poster_IRIIE_2010.pdf Hamzah, Hairul Aini and Mustafa Mahmoud, Mohammed Imad Al-Deen and Abdul Majid, Mohammed Saad and Hasmoni, Mohamed Hadzri and Abdullah, Nasuruddin (2010) Molecular study of hepatitis C viral RNA extracted from local isolates in Pahang, Malaysia: genotyping, subtyping and base sequencing. In: IIUM Research, Innovation & Invention Exhibition (IRIIE 2010), 26 - 27 January 2010, Kuala Lumpur. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QR355 Virology
spellingShingle	QR355 Virology Hamzah, Hairul Aini Mustafa Mahmoud, Mohammed Imad Al-Deen Abdul Majid, Mohammed Saad Hasmoni, Mohamed Hadzri Abdullah, Nasuruddin Molecular study of hepatitis C viral RNA extracted from local isolates in Pahang, Malaysia: genotyping, subtyping and base sequencing
description	Hepatitis C virus infection affects approximately 170 million individuals constituting about 3% of the world's population. Most of those infected face the risk of developing liver cirrhosis and/or liver cancer. In Malaysia, hepatitis C prevalence is 1.6% and is still the foremost infection among multiple blood transfusion groups. The current mainstay treatment of HCV is pegylated alpha-interferon in combination with ribavirin, incurring considerable expense on local health services. In fact, less than 50% of treated patients respond favorably to the given therapy. Understanding the characteristics of the RNA genome of the local HCV genotypes can serve as foundation for future development of rapid diagnostic techniques. In addition, it has the potential for helping in designing small interfering RNA (siRNA) to be utilized in studies related to specific silencing of vital viral genes. However, despite the plethora of global HCV studies, there is relative scarcity HCV research in Malaysia. In this present study, HCV isolates from infected haemodialysis patients were studied, focusing on the characterization of their genomes, by genotyping and base-sequencing. The nucleotide sequence of the conserved 5’UTR region of HCV genome revealed several sequence patterns across the 4 main HCV genotypes available in the study panel. Phylogenetic analysis of the NS5B region showed a predominance of HCV genotype 3a. The revealed sequence patterns have the potential for designing probes that could differentiate the predominant HCV genotype 3 from other genotypes. Analysis of the secondary structure of genotype 3a showed conserved loop structures that could be targeted by small interfering RNA molecules. In conclusion, molecular studies of local HCV strains provide a new dimension for the improvement of current HCV detection and genotyping methods, aid in better understanding of the molecular epidemiology of the virus infection and may form the basis for future in-vitro studies on viral molecular pathogenetic mechanism
format	Conference or Workshop Item
author	Hamzah, Hairul Aini Mustafa Mahmoud, Mohammed Imad Al-Deen Abdul Majid, Mohammed Saad Hasmoni, Mohamed Hadzri Abdullah, Nasuruddin
author_facet	Hamzah, Hairul Aini Mustafa Mahmoud, Mohammed Imad Al-Deen Abdul Majid, Mohammed Saad Hasmoni, Mohamed Hadzri Abdullah, Nasuruddin
author_sort	Hamzah, Hairul Aini
title	Molecular study of hepatitis C viral RNA extracted from local isolates in Pahang, Malaysia: genotyping, subtyping and base sequencing
title_short	Molecular study of hepatitis C viral RNA extracted from local isolates in Pahang, Malaysia: genotyping, subtyping and base sequencing
title_full	Molecular study of hepatitis C viral RNA extracted from local isolates in Pahang, Malaysia: genotyping, subtyping and base sequencing
title_fullStr	Molecular study of hepatitis C viral RNA extracted from local isolates in Pahang, Malaysia: genotyping, subtyping and base sequencing
title_full_unstemmed	Molecular study of hepatitis C viral RNA extracted from local isolates in Pahang, Malaysia: genotyping, subtyping and base sequencing
title_sort	molecular study of hepatitis c viral rna extracted from local isolates in pahang, malaysia: genotyping, subtyping and base sequencing
publishDate	2010
url	http://irep.iium.edu.my/5172/ http://irep.iium.edu.my/5172/1/Poster_IRIIE_2010.pdf
first_indexed	2023-09-18T20:13:39Z
last_indexed	2023-09-18T20:13:39Z
_version_	1777407643185315840

Molecular study of hepatitis C viral RNA extracted from local isolates in Pahang, Malaysia: genotyping, subtyping and base sequencing

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