Screening of β-glucoside kinase directed evolution library to form a sulfur-phosphorus bond

Glycoscience is a field that explores the structures and functions of glycans promising great advances in an array of applications. The objective of this research is to form a sulfur-phosphorus bond at the position 6’ of the sugar involved in many biological processes. A directed evolution strateg...

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Main Authors:	Bellag, Oualid Abdelkader, Ben Belgacem, Farah Fadwa, Noor Batcha, Ibrahim Ali, Mohd Salleh, Hamzah
Format:	Conference or Workshop Item
Language:	English
Published:	Kulliyah of Engineering, International Islamic University Malaysia 2016
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/51693/ http://irep.iium.edu.my/51693/ http://irep.iium.edu.my/51693/1/51693.pdf

id	iium-51693
recordtype	eprints
spelling	iium-516932016-08-19T02:05:15Z http://irep.iium.edu.my/51693/ Screening of β-glucoside kinase directed evolution library to form a sulfur-phosphorus bond Bellag, Oualid Abdelkader Ben Belgacem, Farah Fadwa Noor Batcha, Ibrahim Ali Mohd Salleh, Hamzah TP248.13 Biotechnology Glycoscience is a field that explores the structures and functions of glycans promising great advances in an array of applications. The objective of this research is to form a sulfur-phosphorus bond at the position 6’ of the sugar involved in many biological processes. A directed evolution strategy was adopted with error-prone PCR technique to create random mutagenesis of recombinant plasmid library of the β-glucoside kinase (βglk) recloned into a pTrcHisB plasmid and transformed into Escherichia coli. Following the creation of libraries, high throughput screening by 96-well microplate using fluorescent activated cell sorting (FACS) techniques were carried out. Positive clones were selected, sequenced for identification and enzyme expression and characterization were carried out. Kulliyah of Engineering, International Islamic University Malaysia 2016 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/51693/1/51693.pdf Bellag, Oualid Abdelkader and Ben Belgacem, Farah Fadwa and Noor Batcha, Ibrahim Ali and Mohd Salleh, Hamzah (2016) Screening of β-glucoside kinase directed evolution library to form a sulfur-phosphorus bond. In: 4th International Conference on Biotechnology Engineering 2016 (ICBioE 2016), 25th-27th July 2016, Kuala Lumpur. http://www.iium.edu.my/icbioe/2016/index.php/78-the-4th-international-conference-on-biotechnology-engineering
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Bellag, Oualid Abdelkader Ben Belgacem, Farah Fadwa Noor Batcha, Ibrahim Ali Mohd Salleh, Hamzah Screening of β-glucoside kinase directed evolution library to form a sulfur-phosphorus bond
description	Glycoscience is a field that explores the structures and functions of glycans promising great advances in an array of applications. The objective of this research is to form a sulfur-phosphorus bond at the position 6’ of the sugar involved in many biological processes. A directed evolution strategy was adopted with error-prone PCR technique to create random mutagenesis of recombinant plasmid library of the β-glucoside kinase (βglk) recloned into a pTrcHisB plasmid and transformed into Escherichia coli. Following the creation of libraries, high throughput screening by 96-well microplate using fluorescent activated cell sorting (FACS) techniques were carried out. Positive clones were selected, sequenced for identification and enzyme expression and characterization were carried out.
format	Conference or Workshop Item
author	Bellag, Oualid Abdelkader Ben Belgacem, Farah Fadwa Noor Batcha, Ibrahim Ali Mohd Salleh, Hamzah
author_facet	Bellag, Oualid Abdelkader Ben Belgacem, Farah Fadwa Noor Batcha, Ibrahim Ali Mohd Salleh, Hamzah
author_sort	Bellag, Oualid Abdelkader
title	Screening of β-glucoside kinase directed evolution library to form a sulfur-phosphorus bond
title_short	Screening of β-glucoside kinase directed evolution library to form a sulfur-phosphorus bond
title_full	Screening of β-glucoside kinase directed evolution library to form a sulfur-phosphorus bond
title_fullStr	Screening of β-glucoside kinase directed evolution library to form a sulfur-phosphorus bond
title_full_unstemmed	Screening of β-glucoside kinase directed evolution library to form a sulfur-phosphorus bond
title_sort	screening of β-glucoside kinase directed evolution library to form a sulfur-phosphorus bond
publisher	Kulliyah of Engineering, International Islamic University Malaysia
publishDate	2016
url	http://irep.iium.edu.my/51693/ http://irep.iium.edu.my/51693/ http://irep.iium.edu.my/51693/1/51693.pdf
first_indexed	2023-09-18T21:13:15Z
last_indexed	2023-09-18T21:13:15Z
_version_	1777411392871071744

Screening of β-glucoside kinase directed evolution library to form a sulfur-phosphorus bond

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