Bioprospecting novel cellulose-degrading enzymes from pome metagenomic DNA libraries by enrichment strategy

Green technology is the environment-friendly way to protect the Earth’s natural resources and the environment we inhabit. The functionalmetagenomic approach has proven to be a powerful tool to identify novel biocatalysts indispensables in green technology of biofuel production from plant’s biomass...

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Main Authors:	Ben Belgacem, Farah Fadwa, Bellag, Oualid Abdel Kader, Noor Batcha, Ibrahim Ali, Mohd Salleh , Hamzah
Format:	Conference or Workshop Item
Language:	English
Published:	Kulliyah of Engineering, International Islamic University Malaysia 2016
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/51691/ http://irep.iium.edu.my/51691/ http://irep.iium.edu.my/51691/1/51691.pdf

id	iium-51691
recordtype	eprints
spelling	iium-516912016-08-19T01:50:07Z http://irep.iium.edu.my/51691/ Bioprospecting novel cellulose-degrading enzymes from pome metagenomic DNA libraries by enrichment strategy Ben Belgacem, Farah Fadwa Bellag, Oualid Abdel Kader Noor Batcha, Ibrahim Ali Mohd Salleh , Hamzah TP248.13 Biotechnology Green technology is the environment-friendly way to protect the Earth’s natural resources and the environment we inhabit. The functionalmetagenomic approach has proven to be a powerful tool to identify novel biocatalysts indispensables in green technology of biofuel production from plant’s biomass through metagenomic DNA library construction and high-throughput screening. Culture enrichment strategies are additional pre-screening methods to provide an attractive mean of enhancing the screening hit rate. In this work metagenomic DNA libraries were made from Malaysian palm oil mill effluent (POME) microorganisms after culture enrichment experiment based on natural selection principle. Metagenomic DNA was extracted from both enriched and non-enriched cultures and cloned to pCC1FOS fosmid and transformed into EPI300™-T1RE. coli.Qualitative and quantitative analysis of metagenomic DNA and recombinant clones are provided to confirm the efficiency of enrichment strategy. Kulliyah of Engineering, International Islamic University Malaysia 2016 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/51691/1/51691.pdf Ben Belgacem, Farah Fadwa and Bellag, Oualid Abdel Kader and Noor Batcha, Ibrahim Ali and Mohd Salleh , Hamzah (2016) Bioprospecting novel cellulose-degrading enzymes from pome metagenomic DNA libraries by enrichment strategy. In: 4th International Conference on Biotechnology Engineering 2016 (ICBioE 2016), 25th-27th July 2016, Kuala Lumpur. http://www.iium.edu.my/icbioe/2016/index.php/78-the-4th-international-conference-on-biotechnology-engineering
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Ben Belgacem, Farah Fadwa Bellag, Oualid Abdel Kader Noor Batcha, Ibrahim Ali Mohd Salleh , Hamzah Bioprospecting novel cellulose-degrading enzymes from pome metagenomic DNA libraries by enrichment strategy
description	Green technology is the environment-friendly way to protect the Earth’s natural resources and the environment we inhabit. The functionalmetagenomic approach has proven to be a powerful tool to identify novel biocatalysts indispensables in green technology of biofuel production from plant’s biomass through metagenomic DNA library construction and high-throughput screening. Culture enrichment strategies are additional pre-screening methods to provide an attractive mean of enhancing the screening hit rate. In this work metagenomic DNA libraries were made from Malaysian palm oil mill effluent (POME) microorganisms after culture enrichment experiment based on natural selection principle. Metagenomic DNA was extracted from both enriched and non-enriched cultures and cloned to pCC1FOS fosmid and transformed into EPI300™-T1RE. coli.Qualitative and quantitative analysis of metagenomic DNA and recombinant clones are provided to confirm the efficiency of enrichment strategy.
format	Conference or Workshop Item
author	Ben Belgacem, Farah Fadwa Bellag, Oualid Abdel Kader Noor Batcha, Ibrahim Ali Mohd Salleh , Hamzah
author_facet	Ben Belgacem, Farah Fadwa Bellag, Oualid Abdel Kader Noor Batcha, Ibrahim Ali Mohd Salleh , Hamzah
author_sort	Ben Belgacem, Farah Fadwa
title	Bioprospecting novel cellulose-degrading enzymes from pome metagenomic DNA libraries by enrichment strategy
title_short	Bioprospecting novel cellulose-degrading enzymes from pome metagenomic DNA libraries by enrichment strategy
title_full	Bioprospecting novel cellulose-degrading enzymes from pome metagenomic DNA libraries by enrichment strategy
title_fullStr	Bioprospecting novel cellulose-degrading enzymes from pome metagenomic DNA libraries by enrichment strategy
title_full_unstemmed	Bioprospecting novel cellulose-degrading enzymes from pome metagenomic DNA libraries by enrichment strategy
title_sort	bioprospecting novel cellulose-degrading enzymes from pome metagenomic dna libraries by enrichment strategy
publisher	Kulliyah of Engineering, International Islamic University Malaysia
publishDate	2016
url	http://irep.iium.edu.my/51691/ http://irep.iium.edu.my/51691/ http://irep.iium.edu.my/51691/1/51691.pdf
first_indexed	2023-09-18T21:13:14Z
last_indexed	2023-09-18T21:13:14Z
_version_	1777411392573276160

Bioprospecting novel cellulose-degrading enzymes from pome metagenomic DNA libraries by enrichment strategy

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