Bioprospecting novel cellulose-degrading enzymes from pome metagenomic DNA libraries by enrichment strategy
Green technology is the environment-friendly way to protect the Earth’s natural resources and the environment we inhabit. The functionalmetagenomic approach has proven to be a powerful tool to identify novel biocatalysts indispensables in green technology of biofuel production from plant’s biomass...
| Main Authors: | , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
Kulliyah of Engineering, International Islamic University Malaysia
2016
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/51691/ http://irep.iium.edu.my/51691/ http://irep.iium.edu.my/51691/1/51691.pdf |
| Summary: | Green technology is the environment-friendly way to protect the Earth’s natural resources and the
environment we inhabit. The functionalmetagenomic approach has proven to be a powerful tool to identify
novel biocatalysts indispensables in green technology of biofuel production from plant’s biomass through
metagenomic DNA library construction and high-throughput screening. Culture enrichment strategies are
additional pre-screening methods to provide an attractive mean of enhancing the screening hit rate. In this
work metagenomic DNA libraries were made from Malaysian palm oil mill effluent (POME) microorganisms
after culture enrichment experiment based on natural selection principle. Metagenomic DNA was extracted
from both enriched and non-enriched cultures and cloned to pCC1FOS fosmid and transformed into
EPI300™-T1RE. coli.Qualitative and quantitative analysis of metagenomic DNA and recombinant clones are
provided to confirm the efficiency of enrichment strategy. |
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