Characterization of poly (lactic-co-glycolic acid) / atelocollagen hybrid scaffold for intervertebral disc regeneration

Characterization of poly (lactic-co-glycolic acid) / atelocollagen hybrid scaffold for intervertebral disc regeneration

The objective of the present study is to characterize PLGA and atelocollagen type 2 (PLGA-A) hybrid scaffolds in terms of swelling capacity, thermal properties and mechanical strength. PLGA without atelocollagen was used as control.Porous PLGA scaffold is fabricated via salt leaching/ solvent-cast...

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Main Authors: Mohamad, Mohd Yusof, Mohamed Amin, Muhammad Azri Ifwat, Md Nazir, Noorhidayah, Ahmad Radzi, Muhammad Aa’zamuddin, Hashim, Rosyafirah, Mat Nawi, Nur Farhana, Zulkifly, Ahmad Hafiz, Sha’ban, Munirah
Format: Conference or Workshop Item
Language:English
Published: Kulliyah of Engineering, International Islamic University Malaysia 2016
Subjects:
R Medicine (General)
TP248.13 Biotechnology
Online Access:http://irep.iium.edu.my/51690/
http://irep.iium.edu.my/51690/
http://irep.iium.edu.my/51690/1/51690.pdf
id iium-51690
recordtype eprints
spelling iium-516902016-08-19T00:49:33Z http://irep.iium.edu.my/51690/ Characterization of poly (lactic-co-glycolic acid) / atelocollagen hybrid scaffold for intervertebral disc regeneration Mohamad, Mohd Yusof Mohamed Amin, Muhammad Azri Ifwat Md Nazir, Noorhidayah Ahmad Radzi, Muhammad Aa’zamuddin Hashim, Rosyafirah Mat Nawi, Nur Farhana Zulkifly, Ahmad Hafiz Sha’ban, Munirah R Medicine (General) TP248.13 Biotechnology The objective of the present study is to characterize PLGA and atelocollagen type 2 (PLGA-A) hybrid scaffolds in terms of swelling capacity, thermal properties and mechanical strength. PLGA without atelocollagen was used as control.Porous PLGA scaffold is fabricated via salt leaching/ solvent-casting using salt at 350-400 micron. 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and N-Hydroxysuccinimide (NHS) were used to crosslink atelocollagen with PLGA. The incorporation of atelocollagen and PLGA is confirmed by the presence of amide formation of amide bonds at 1650cm-1 using Fourier transform infrared spectroscopy (ATR-FTIR). Scanning Electron Microscopy (SEM) images demonstrated addition of atelocollagen did not jeopardize porous structure of PLGA scaffolds. Differential Scanning Calorimetry (DSC) analysis showed increment of glass transition, Tg of PLGA-A by 13°C. The swelling ratio of PLGA-A scaffold showed 4.8 times increase water uptake within 24-hour period. The mechanical strength of PLGA-A is enhanced by 255.95% when tested with Instron E3000 machine. These findings suggest PLGA incorporation with atelocollagen type 2 may become a promising biomaterial for intervertebral disc regeneration. Kulliyah of Engineering, International Islamic University Malaysia 2016 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/51690/1/51690.pdf Mohamad, Mohd Yusof and Mohamed Amin, Muhammad Azri Ifwat and Md Nazir, Noorhidayah and Ahmad Radzi, Muhammad Aa’zamuddin and Hashim, Rosyafirah and Mat Nawi, Nur Farhana and Zulkifly, Ahmad Hafiz and Sha’ban, Munirah (2016) Characterization of poly (lactic-co-glycolic acid) / atelocollagen hybrid scaffold for intervertebral disc regeneration. In: 4th International Conference on Biotechnology Engineering 2016 (ICBioE 2016), 25th-27th July 2016, Kuala Lumpur. http://www.iium.edu.my/icbioe/2016/index.php/78-the-4th-international-conference-on-biotechnology-engineering
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic R Medicine (General)
TP248.13 Biotechnology
spellingShingle R Medicine (General)
TP248.13 Biotechnology
Mohamad, Mohd Yusof
Mohamed Amin, Muhammad Azri Ifwat
Md Nazir, Noorhidayah
Ahmad Radzi, Muhammad Aa’zamuddin
Hashim, Rosyafirah
Mat Nawi, Nur Farhana
Zulkifly, Ahmad Hafiz
Sha’ban, Munirah
Characterization of poly (lactic-co-glycolic acid) / atelocollagen hybrid scaffold for intervertebral disc regeneration
description The objective of the present study is to characterize PLGA and atelocollagen type 2 (PLGA-A) hybrid scaffolds in terms of swelling capacity, thermal properties and mechanical strength. PLGA without atelocollagen was used as control.Porous PLGA scaffold is fabricated via salt leaching/ solvent-casting using salt at 350-400 micron. 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and N-Hydroxysuccinimide (NHS) were used to crosslink atelocollagen with PLGA. The incorporation of atelocollagen and PLGA is confirmed by the presence of amide formation of amide bonds at 1650cm-1 using Fourier transform infrared spectroscopy (ATR-FTIR). Scanning Electron Microscopy (SEM) images demonstrated addition of atelocollagen did not jeopardize porous structure of PLGA scaffolds. Differential Scanning Calorimetry (DSC) analysis showed increment of glass transition, Tg of PLGA-A by 13°C. The swelling ratio of PLGA-A scaffold showed 4.8 times increase water uptake within 24-hour period. The mechanical strength of PLGA-A is enhanced by 255.95% when tested with Instron E3000 machine. These findings suggest PLGA incorporation with atelocollagen type 2 may become a promising biomaterial for intervertebral disc regeneration.
format Conference or Workshop Item
author Mohamad, Mohd Yusof
Mohamed Amin, Muhammad Azri Ifwat
Md Nazir, Noorhidayah
Ahmad Radzi, Muhammad Aa’zamuddin
Hashim, Rosyafirah
Mat Nawi, Nur Farhana
Zulkifly, Ahmad Hafiz
Sha’ban, Munirah
author_facet Mohamad, Mohd Yusof
Mohamed Amin, Muhammad Azri Ifwat
Md Nazir, Noorhidayah
Ahmad Radzi, Muhammad Aa’zamuddin
Hashim, Rosyafirah
Mat Nawi, Nur Farhana
Zulkifly, Ahmad Hafiz
Sha’ban, Munirah
author_sort Mohamad, Mohd Yusof
title Characterization of poly (lactic-co-glycolic acid) / atelocollagen hybrid scaffold for intervertebral disc regeneration
title_short Characterization of poly (lactic-co-glycolic acid) / atelocollagen hybrid scaffold for intervertebral disc regeneration
title_full Characterization of poly (lactic-co-glycolic acid) / atelocollagen hybrid scaffold for intervertebral disc regeneration
title_fullStr Characterization of poly (lactic-co-glycolic acid) / atelocollagen hybrid scaffold for intervertebral disc regeneration
title_full_unstemmed Characterization of poly (lactic-co-glycolic acid) / atelocollagen hybrid scaffold for intervertebral disc regeneration
title_sort characterization of poly (lactic-co-glycolic acid) / atelocollagen hybrid scaffold for intervertebral disc regeneration
publisher Kulliyah of Engineering, International Islamic University Malaysia
publishDate 2016
url http://irep.iium.edu.my/51690/
http://irep.iium.edu.my/51690/
http://irep.iium.edu.my/51690/1/51690.pdf
first_indexed 2023-09-18T21:13:14Z
last_indexed 2023-09-18T21:13:14Z
_version_ 1777411392421232640

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