Characterization of poly (lactic-co-glycolic acid) / atelocollagen hybrid scaffold for intervertebral disc regeneration
The objective of the present study is to characterize PLGA and atelocollagen type 2 (PLGA-A) hybrid scaffolds in terms of swelling capacity, thermal properties and mechanical strength. PLGA without atelocollagen was used as control.Porous PLGA scaffold is fabricated via salt leaching/ solvent-cast...
| Main Authors: | , , , , , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
Kulliyah of Engineering, International Islamic University Malaysia
2016
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/51690/ http://irep.iium.edu.my/51690/ http://irep.iium.edu.my/51690/1/51690.pdf |
| Summary: | The objective of the present study is to characterize PLGA and atelocollagen type 2 (PLGA-A) hybrid
scaffolds in terms of swelling capacity, thermal properties and mechanical strength. PLGA without
atelocollagen was used as control.Porous PLGA scaffold is fabricated via salt leaching/ solvent-casting using
salt at 350-400 micron. 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and N-Hydroxysuccinimide
(NHS) were used to crosslink atelocollagen with PLGA. The incorporation of atelocollagen and PLGA is
confirmed by the presence of amide formation of amide bonds at 1650cm-1 using Fourier transform infrared
spectroscopy (ATR-FTIR). Scanning Electron Microscopy (SEM) images demonstrated addition of
atelocollagen did not jeopardize porous structure of PLGA scaffolds. Differential Scanning Calorimetry
(DSC) analysis showed increment of glass transition, Tg of PLGA-A by 13°C. The swelling ratio of PLGA-A
scaffold showed 4.8 times increase water uptake within 24-hour period. The mechanical strength of PLGA-A
is enhanced by 255.95% when tested with Instron E3000 machine. These findings suggest PLGA
incorporation with atelocollagen type 2 may become a promising biomaterial for intervertebral disc
regeneration. |
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