Screening of thermostable and organic solvent tolerant lipase producing fungi in solid state bioconversion of palm kernel cake

Special consideration has been focused on the utilization of lipase enzyme as a suitable alternative method for biodiesel production, which is expected to be more stable and practical. However, the high production costs as well as low productivity have hindered the marketable production of biodies...

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Main Authors:	Riyadi, Fatimah Azizah, Alam, Md. Zahangir, Salleh, Md Noor, Mohd Salleh, Hamzah
Format:	Conference or Workshop Item
Language:	English
Published:	Kulliyah of Engineering, International Islamic University Malaysia 2016
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/51681/ http://irep.iium.edu.my/51681/1/51681.pdf

id	iium-51681
recordtype	eprints
spelling	iium-516812018-02-26T08:12:44Z http://irep.iium.edu.my/51681/ Screening of thermostable and organic solvent tolerant lipase producing fungi in solid state bioconversion of palm kernel cake Riyadi, Fatimah Azizah Alam, Md. Zahangir Salleh, Md Noor Mohd Salleh, Hamzah TP248.13 Biotechnology Special consideration has been focused on the utilization of lipase enzyme as a suitable alternative method for biodiesel production, which is expected to be more stable and practical. However, the high production costs as well as low productivity have hindered the marketable production of biodiesel using the commercial lipase. Isolation of lipases from filamentous fungi, particularly found in agricultural wastes has attracted special interest due to their potential towards developing more sustainable and economical applications. Only lipase possessing thermal stability and organic solvent tolerant can distinctively enhance the biodiesel production. Therefore, in the present study, isolation of thermostable- organic solvent tolerant fungal strains from palm kernel cake (PKC) were conducted based on their ability to grow at 45oC on YpSs (yeast extract + starch + agar) media and in the presence of organic solvent. The isolates were screened based on the clearing zone methods on phenol red as well as on tween 80 agar media. Four isolates were screened for potential lipase production. These isolates were incubated under Solid State Fermentation (SSF) at 45oC for 7 days with PKC as substrate. The most promising strain with the potential to produce lipase was PKC12B2, which showed satisfactory enzyme activity. Kulliyah of Engineering, International Islamic University Malaysia 2016 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/51681/1/51681.pdf Riyadi, Fatimah Azizah and Alam, Md. Zahangir and Salleh, Md Noor and Mohd Salleh, Hamzah (2016) Screening of thermostable and organic solvent tolerant lipase producing fungi in solid state bioconversion of palm kernel cake. In: 4th International Conference on Biotechnology Engineering 2016 (ICBioE 2016), 25th-27th July 2016, Kuala Lumpur.
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Riyadi, Fatimah Azizah Alam, Md. Zahangir Salleh, Md Noor Mohd Salleh, Hamzah Screening of thermostable and organic solvent tolerant lipase producing fungi in solid state bioconversion of palm kernel cake
description	Special consideration has been focused on the utilization of lipase enzyme as a suitable alternative method for biodiesel production, which is expected to be more stable and practical. However, the high production costs as well as low productivity have hindered the marketable production of biodiesel using the commercial lipase. Isolation of lipases from filamentous fungi, particularly found in agricultural wastes has attracted special interest due to their potential towards developing more sustainable and economical applications. Only lipase possessing thermal stability and organic solvent tolerant can distinctively enhance the biodiesel production. Therefore, in the present study, isolation of thermostable- organic solvent tolerant fungal strains from palm kernel cake (PKC) were conducted based on their ability to grow at 45oC on YpSs (yeast extract + starch + agar) media and in the presence of organic solvent. The isolates were screened based on the clearing zone methods on phenol red as well as on tween 80 agar media. Four isolates were screened for potential lipase production. These isolates were incubated under Solid State Fermentation (SSF) at 45oC for 7 days with PKC as substrate. The most promising strain with the potential to produce lipase was PKC12B2, which showed satisfactory enzyme activity.
format	Conference or Workshop Item
author	Riyadi, Fatimah Azizah Alam, Md. Zahangir Salleh, Md Noor Mohd Salleh, Hamzah
author_facet	Riyadi, Fatimah Azizah Alam, Md. Zahangir Salleh, Md Noor Mohd Salleh, Hamzah
author_sort	Riyadi, Fatimah Azizah
title	Screening of thermostable and organic solvent tolerant lipase producing fungi in solid state bioconversion of palm kernel cake
title_short	Screening of thermostable and organic solvent tolerant lipase producing fungi in solid state bioconversion of palm kernel cake
title_full	Screening of thermostable and organic solvent tolerant lipase producing fungi in solid state bioconversion of palm kernel cake
title_fullStr	Screening of thermostable and organic solvent tolerant lipase producing fungi in solid state bioconversion of palm kernel cake
title_full_unstemmed	Screening of thermostable and organic solvent tolerant lipase producing fungi in solid state bioconversion of palm kernel cake
title_sort	screening of thermostable and organic solvent tolerant lipase producing fungi in solid state bioconversion of palm kernel cake
publisher	Kulliyah of Engineering, International Islamic University Malaysia
publishDate	2016
url	http://irep.iium.edu.my/51681/ http://irep.iium.edu.my/51681/1/51681.pdf
first_indexed	2023-09-18T21:13:13Z
last_indexed	2023-09-18T21:13:13Z
_version_	1777411391128338432

Screening of thermostable and organic solvent tolerant lipase producing fungi in solid state bioconversion of palm kernel cake

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