Statistical optimization of gelatin immobilization to improve PCL microcarrier surface

Growing cells on microcarriers may have overcome the limitation of conventional cell culture system. However, the main challenge remains at ensuring the surface biocompatibility with cells. Polycaprolactone (PCL), a biodegradable polymer, has received considerable attention because of its excellent...

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Main Authors:	Samsudin, Nurhusna, Hashim, Yumi Zuhanis Has-Yun, Arifin, Mohd Azmir, Mel, Maizirwan, Mohd. Salleh, Hamzah, Sopyan, Iis, Jimat, Dzun Noraini
Format:	Conference or Workshop Item
Language:	English
Published:	Kulliyyah of Engineering, International Islamic University Malaysia 2016
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/51646/ http://irep.iium.edu.my/51646/ http://irep.iium.edu.my/51646/1/51646_Statistical_optimization_of_gelatin.pdf

id	iium-51646
recordtype	eprints
spelling	iium-516462017-10-19T06:41:32Z http://irep.iium.edu.my/51646/ Statistical optimization of gelatin immobilization to improve PCL microcarrier surface Samsudin, Nurhusna Hashim, Yumi Zuhanis Has-Yun Arifin, Mohd Azmir Mel, Maizirwan Mohd. Salleh, Hamzah Sopyan, Iis Jimat, Dzun Noraini Q Science (General) Growing cells on microcarriers may have overcome the limitation of conventional cell culture system. However, the main challenge remains at ensuring the surface biocompatibility with cells. Polycaprolactone (PCL), a biodegradable polymer, has received considerable attention because of its excellent mechanical properties and degradation kinetics that suit various applications, but its non-polar hydrocarbon moiety renders it sub-optimal for cell attachment. In this present study, oxygen functional group was introduced via ultraviolet irradiation and ozone aeration (UV/O3) system to allow covalent immobilization of gelatin on the PCL microcarrier surface using zero-length crosslinker reagent 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxysuccinimide (EDAC/NHS). The optimum conditions for maximizing the amount of gelatin on the surface of PCL were as follows: 1.5 of COOH:EDAC ratio, 10mM NHS concentration and, 80 mg/ml gelatin. Kulliyyah of Engineering, International Islamic University Malaysia 2016 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/51646/1/51646_Statistical_optimization_of_gelatin.pdf Samsudin, Nurhusna and Hashim, Yumi Zuhanis Has-Yun and Arifin, Mohd Azmir and Mel, Maizirwan and Mohd. Salleh, Hamzah and Sopyan, Iis and Jimat, Dzun Noraini (2016) Statistical optimization of gelatin immobilization to improve PCL microcarrier surface. In: 4th International Conference on Biotechnology Engineering 2016 (ICBioE 2016), 25th-27th July 2016, Kuala Lumpur. http://www.iium.edu.my/icbioe/2016/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Samsudin, Nurhusna Hashim, Yumi Zuhanis Has-Yun Arifin, Mohd Azmir Mel, Maizirwan Mohd. Salleh, Hamzah Sopyan, Iis Jimat, Dzun Noraini Statistical optimization of gelatin immobilization to improve PCL microcarrier surface
description	Growing cells on microcarriers may have overcome the limitation of conventional cell culture system. However, the main challenge remains at ensuring the surface biocompatibility with cells. Polycaprolactone (PCL), a biodegradable polymer, has received considerable attention because of its excellent mechanical properties and degradation kinetics that suit various applications, but its non-polar hydrocarbon moiety renders it sub-optimal for cell attachment. In this present study, oxygen functional group was introduced via ultraviolet irradiation and ozone aeration (UV/O3) system to allow covalent immobilization of gelatin on the PCL microcarrier surface using zero-length crosslinker reagent 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxysuccinimide (EDAC/NHS). The optimum conditions for maximizing the amount of gelatin on the surface of PCL were as follows: 1.5 of COOH:EDAC ratio, 10mM NHS concentration and, 80 mg/ml gelatin.
format	Conference or Workshop Item
author	Samsudin, Nurhusna Hashim, Yumi Zuhanis Has-Yun Arifin, Mohd Azmir Mel, Maizirwan Mohd. Salleh, Hamzah Sopyan, Iis Jimat, Dzun Noraini
author_facet	Samsudin, Nurhusna Hashim, Yumi Zuhanis Has-Yun Arifin, Mohd Azmir Mel, Maizirwan Mohd. Salleh, Hamzah Sopyan, Iis Jimat, Dzun Noraini
author_sort	Samsudin, Nurhusna
title	Statistical optimization of gelatin immobilization to improve PCL microcarrier surface
title_short	Statistical optimization of gelatin immobilization to improve PCL microcarrier surface
title_full	Statistical optimization of gelatin immobilization to improve PCL microcarrier surface
title_fullStr	Statistical optimization of gelatin immobilization to improve PCL microcarrier surface
title_full_unstemmed	Statistical optimization of gelatin immobilization to improve PCL microcarrier surface
title_sort	statistical optimization of gelatin immobilization to improve pcl microcarrier surface
publisher	Kulliyyah of Engineering, International Islamic University Malaysia
publishDate	2016
url	http://irep.iium.edu.my/51646/ http://irep.iium.edu.my/51646/ http://irep.iium.edu.my/51646/1/51646_Statistical_optimization_of_gelatin.pdf
first_indexed	2023-09-18T21:13:08Z
last_indexed	2023-09-18T21:13:08Z
_version_	1777411386477903872

Statistical optimization of gelatin immobilization to improve PCL microcarrier surface

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