Cytokinetic study of uninfected agarwood branch ethanolic extract on breast cancer cells

Breast cancer is the most common malignancy in women with more than 500,000 global death toll annually. Evidences from ethnopharmaceutical practices showed that Aquilaria spp. from Thymelaeceae family has been used traditionally as treatment of a wide range of disorders. However, scientific evidence...

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Main Authors:	Abbas, Phirdaous, Hashim, Yumi Zuhanis Has-Yun, Amid, Azura
Format:	Conference or Workshop Item
Language:	English
Published:	Kulliyyah of Engineering, International Islamic University Malaysia 2016
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/51642/ http://irep.iium.edu.my/51642/ http://irep.iium.edu.my/51642/1/51642_Cytokinetic_study_of_uninfected_agarwood_branch.pdf

id	iium-51642
recordtype	eprints
spelling	iium-516422017-01-16T02:30:27Z http://irep.iium.edu.my/51642/ Cytokinetic study of uninfected agarwood branch ethanolic extract on breast cancer cells Abbas, Phirdaous Hashim, Yumi Zuhanis Has-Yun Amid, Azura Q Science (General) Breast cancer is the most common malignancy in women with more than 500,000 global death toll annually. Evidences from ethnopharmaceutical practices showed that Aquilaria spp. from Thymelaeceae family has been used traditionally as treatment of a wide range of disorders. However, scientific evidences are still lacking in order to support the traditional applications on cancer disease. This study was aimed to investigate the cytotoxic kinetics of uninfected agarwood branch ethanolic extract from Aquilaria subintegra against MCF-7 breast cancer cell line that may help understand the effect and provide baseline towards development of agarwood-based therapeutics. Screening assays conducted revealed that the extract may possess anti-attachment and cytotoxic effects against MCF-7 breast cancer cells with a significant inhibition concentration (IC50) of 8 μg/ml when tested using sulforhodamine B (SRB) assay. Extract was subjected to a series of kinetic study in order to investigate the growth rate, death rate, doubling time and death time of MCF-7 between the treated and control groups. Experiments were conducted in triplicates and results obtained showed that the extract reduced the cell generation number of MCF-7 from 3.52 to 1.22 and the growth rate from 0.0421 h-1 to 0.0158 h-1. Doubling time of MCF-7 cells was extended from 16.4 hours to 43.8 hours. The death rate was increased from 0.0058 h-1 to 0.0563 h-1. Closer observation on the population density images revealed that the extract-treated MCF-7 cells showed irregular appearance with blebs, volume reduction, cell shrinking and loss of cell-cell contact. Data collected made it possible to develop an early model of cancer dose treatment using the first order linear equation as a function of time that can be used to estimate cell size or volume of cancer, speed of growth and possibility of successful dosage/treatments using data from biopsy procedure. These findings suggested that agarwood uninfected branch ethanolic extract affect the cytokinetics of MCF-7 cells which warrants further research and focus in validating the hypothesis and elucidating the effects against cancer. Kulliyyah of Engineering, International Islamic University Malaysia 2016 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/51642/1/51642_Cytokinetic_study_of_uninfected_agarwood_branch.pdf Abbas, Phirdaous and Hashim, Yumi Zuhanis Has-Yun and Amid, Azura (2016) Cytokinetic study of uninfected agarwood branch ethanolic extract on breast cancer cells. In: 4th International Conference on Biotechnology Engineering 2016 (ICBioE 2016), 25th-27th July 2016, Kuala Lumpur. http://www.iium.edu.my/icbioe/2016/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Abbas, Phirdaous Hashim, Yumi Zuhanis Has-Yun Amid, Azura Cytokinetic study of uninfected agarwood branch ethanolic extract on breast cancer cells
description	Breast cancer is the most common malignancy in women with more than 500,000 global death toll annually. Evidences from ethnopharmaceutical practices showed that Aquilaria spp. from Thymelaeceae family has been used traditionally as treatment of a wide range of disorders. However, scientific evidences are still lacking in order to support the traditional applications on cancer disease. This study was aimed to investigate the cytotoxic kinetics of uninfected agarwood branch ethanolic extract from Aquilaria subintegra against MCF-7 breast cancer cell line that may help understand the effect and provide baseline towards development of agarwood-based therapeutics. Screening assays conducted revealed that the extract may possess anti-attachment and cytotoxic effects against MCF-7 breast cancer cells with a significant inhibition concentration (IC50) of 8 μg/ml when tested using sulforhodamine B (SRB) assay. Extract was subjected to a series of kinetic study in order to investigate the growth rate, death rate, doubling time and death time of MCF-7 between the treated and control groups. Experiments were conducted in triplicates and results obtained showed that the extract reduced the cell generation number of MCF-7 from 3.52 to 1.22 and the growth rate from 0.0421 h-1 to 0.0158 h-1. Doubling time of MCF-7 cells was extended from 16.4 hours to 43.8 hours. The death rate was increased from 0.0058 h-1 to 0.0563 h-1. Closer observation on the population density images revealed that the extract-treated MCF-7 cells showed irregular appearance with blebs, volume reduction, cell shrinking and loss of cell-cell contact. Data collected made it possible to develop an early model of cancer dose treatment using the first order linear equation as a function of time that can be used to estimate cell size or volume of cancer, speed of growth and possibility of successful dosage/treatments using data from biopsy procedure. These findings suggested that agarwood uninfected branch ethanolic extract affect the cytokinetics of MCF-7 cells which warrants further research and focus in validating the hypothesis and elucidating the effects against cancer.
format	Conference or Workshop Item
author	Abbas, Phirdaous Hashim, Yumi Zuhanis Has-Yun Amid, Azura
author_facet	Abbas, Phirdaous Hashim, Yumi Zuhanis Has-Yun Amid, Azura
author_sort	Abbas, Phirdaous
title	Cytokinetic study of uninfected agarwood branch ethanolic extract on breast cancer cells
title_short	Cytokinetic study of uninfected agarwood branch ethanolic extract on breast cancer cells
title_full	Cytokinetic study of uninfected agarwood branch ethanolic extract on breast cancer cells
title_fullStr	Cytokinetic study of uninfected agarwood branch ethanolic extract on breast cancer cells
title_full_unstemmed	Cytokinetic study of uninfected agarwood branch ethanolic extract on breast cancer cells
title_sort	cytokinetic study of uninfected agarwood branch ethanolic extract on breast cancer cells
publisher	Kulliyyah of Engineering, International Islamic University Malaysia
publishDate	2016
url	http://irep.iium.edu.my/51642/ http://irep.iium.edu.my/51642/ http://irep.iium.edu.my/51642/1/51642_Cytokinetic_study_of_uninfected_agarwood_branch.pdf
first_indexed	2023-09-18T21:13:08Z
last_indexed	2023-09-18T21:13:08Z
_version_	1777411386183254016

Cytokinetic study of uninfected agarwood branch ethanolic extract on breast cancer cells

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