The role of polyamines in growth inhibition induced by Momordica charantia and Gynura procumbens in human lung adenocarcinoma cells, A549

Chemoprevention is a crucial process in overcoming the cancer burden throughout the world. By changing dietary choices, people who are at high risk of developing cancer may slow down the carcinogenesis or preventing it altogether. Some food contains high level of polyamine which is bad due to its ab...

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Main Authors:	AhmadSujangi, Nurhikmah, Abdullah, Nor Zamzila, Abdul Ghani, Radiah
Format:	Conference or Workshop Item
Language:	English English
Published:	2016
Subjects:	RM214 Diet therapy. Diet and dietectics in disease
Online Access:	http://irep.iium.edu.my/51635/ http://irep.iium.edu.my/51635/3/POSTER_ICIP_2016.pdf http://irep.iium.edu.my/51635/10/51635_The_role_of_polyamines_in_growth.pdf

id	iium-51635
recordtype	eprints
spelling	iium-516352016-08-17T00:58:49Z http://irep.iium.edu.my/51635/ The role of polyamines in growth inhibition induced by Momordica charantia and Gynura procumbens in human lung adenocarcinoma cells, A549 AhmadSujangi, Nurhikmah Abdullah, Nor Zamzila Abdul Ghani, Radiah RM214 Diet therapy. Diet and dietectics in disease Chemoprevention is a crucial process in overcoming the cancer burden throughout the world. By changing dietary choices, people who are at high risk of developing cancer may slow down the carcinogenesis or preventing it altogether. Some food contains high level of polyamine which is bad due to its ability for promoting tumor growth. Hence, this research aimed to determine the polyamine content of selected local plants, Momordica charantia and Gynura procumbens, by using High Performance Liquid Chromatographic (HPLC) analysis as well as conducting cytotoxicity study and growth inhibition study of the plant extracts against the human lung adenocarcinoma epithelial cell lines, A549. The polyamine content of M. charantia was found to be high (more than 200 nmol/g/mol) while G. procumbens contained low level of polyamine (less than 100 nmol/g/ml). Both plants induced cytotoxicity to A549 cells after only 24 hours of exposure to different concentrations of treatment which lead to the establishment of the IC50 at 10.0 mg/ml. Growth curve analysis demonstrated that M. charantia and G. procumbens had successfully reduced the cancer cell progression. On the other hand, there was an increase of intracellular polyamines in the treated cells compared to untreated cells for both M. charantia and G. procumbens. Based on these preliminary findings, it can be concluded that M. charantia and G. procumbens are not an excellent plants to be included in the development of chemoprevention strategy as the ideal chemopreventive agents are those that contain low polyamine level, able to reduce the cancer cell progression and can inhibit the biosynthesis of polyamine in cancer cells to avoid recurrence of cancer. However, further investigation on the effect of these plants on polyamine metabolic pathway and cell death pathway shall be elucidated. 2016-08-10 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/51635/3/POSTER_ICIP_2016.pdf application/pdf en http://irep.iium.edu.my/51635/10/51635_The_role_of_polyamines_in_growth.pdf AhmadSujangi, Nurhikmah and Abdullah, Nor Zamzila and Abdul Ghani, Radiah (2016) The role of polyamines in growth inhibition induced by Momordica charantia and Gynura procumbens in human lung adenocarcinoma cells, A549. In: 2nd International Conference on Industrial Pharmacy (ICIP 2016), 15th-16th Aug. 2016, Kuantan, Pahang. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RM214 Diet therapy. Diet and dietectics in disease
spellingShingle	RM214 Diet therapy. Diet and dietectics in disease AhmadSujangi, Nurhikmah Abdullah, Nor Zamzila Abdul Ghani, Radiah The role of polyamines in growth inhibition induced by Momordica charantia and Gynura procumbens in human lung adenocarcinoma cells, A549
description	Chemoprevention is a crucial process in overcoming the cancer burden throughout the world. By changing dietary choices, people who are at high risk of developing cancer may slow down the carcinogenesis or preventing it altogether. Some food contains high level of polyamine which is bad due to its ability for promoting tumor growth. Hence, this research aimed to determine the polyamine content of selected local plants, Momordica charantia and Gynura procumbens, by using High Performance Liquid Chromatographic (HPLC) analysis as well as conducting cytotoxicity study and growth inhibition study of the plant extracts against the human lung adenocarcinoma epithelial cell lines, A549. The polyamine content of M. charantia was found to be high (more than 200 nmol/g/mol) while G. procumbens contained low level of polyamine (less than 100 nmol/g/ml). Both plants induced cytotoxicity to A549 cells after only 24 hours of exposure to different concentrations of treatment which lead to the establishment of the IC50 at 10.0 mg/ml. Growth curve analysis demonstrated that M. charantia and G. procumbens had successfully reduced the cancer cell progression. On the other hand, there was an increase of intracellular polyamines in the treated cells compared to untreated cells for both M. charantia and G. procumbens. Based on these preliminary findings, it can be concluded that M. charantia and G. procumbens are not an excellent plants to be included in the development of chemoprevention strategy as the ideal chemopreventive agents are those that contain low polyamine level, able to reduce the cancer cell progression and can inhibit the biosynthesis of polyamine in cancer cells to avoid recurrence of cancer. However, further investigation on the effect of these plants on polyamine metabolic pathway and cell death pathway shall be elucidated.
format	Conference or Workshop Item
author	AhmadSujangi, Nurhikmah Abdullah, Nor Zamzila Abdul Ghani, Radiah
author_facet	AhmadSujangi, Nurhikmah Abdullah, Nor Zamzila Abdul Ghani, Radiah
author_sort	AhmadSujangi, Nurhikmah
title	The role of polyamines in growth inhibition induced by Momordica charantia and Gynura procumbens in human lung adenocarcinoma cells, A549
title_short	The role of polyamines in growth inhibition induced by Momordica charantia and Gynura procumbens in human lung adenocarcinoma cells, A549
title_full	The role of polyamines in growth inhibition induced by Momordica charantia and Gynura procumbens in human lung adenocarcinoma cells, A549
title_fullStr	The role of polyamines in growth inhibition induced by Momordica charantia and Gynura procumbens in human lung adenocarcinoma cells, A549
title_full_unstemmed	The role of polyamines in growth inhibition induced by Momordica charantia and Gynura procumbens in human lung adenocarcinoma cells, A549
title_sort	role of polyamines in growth inhibition induced by momordica charantia and gynura procumbens in human lung adenocarcinoma cells, a549
publishDate	2016
url	http://irep.iium.edu.my/51635/ http://irep.iium.edu.my/51635/3/POSTER_ICIP_2016.pdf http://irep.iium.edu.my/51635/10/51635_The_role_of_polyamines_in_growth.pdf
first_indexed	2023-09-18T21:13:07Z
last_indexed	2023-09-18T21:13:07Z
_version_	1777411385345441792

The role of polyamines in growth inhibition induced by Momordica charantia and Gynura procumbens in human lung adenocarcinoma cells, A549

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