The effects of plant growth regulators to Citrus suhuiensis callus induction

The diseases hampering Citrus suhuiensis (C. suhuiensis) or commonly known as ‘limau madu’ in East Asia has become a major problem to their cultivation and thus, plant cell culture techniques can be one of the solution to overcome this problem. Plant growth regulators (PGRs) play important role in i...

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Main Authors:	M. Fathil, Nur Alia, Mohamad Puad, Noor Illi, Amid, Azura, Azmi, Azlin Suhaida, Ibrahim, Rusli
Format:	Conference or Workshop Item
Language:	English
Published:	Kulliyyah of Engineering, International Islamic University Malaysia 2016
Subjects:	SB Plant culture
Online Access:	http://irep.iium.edu.my/51634/ http://irep.iium.edu.my/51634/6/51634.pdf

id	iium-51634
recordtype	eprints
spelling	iium-516342016-08-16T07:03:58Z http://irep.iium.edu.my/51634/ The effects of plant growth regulators to Citrus suhuiensis callus induction M. Fathil, Nur Alia Mohamad Puad, Noor Illi Amid, Azura Azmi, Azlin Suhaida Ibrahim, Rusli SB Plant culture The diseases hampering Citrus suhuiensis (C. suhuiensis) or commonly known as ‘limau madu’ in East Asia has become a major problem to their cultivation and thus, plant cell culture techniques can be one of the solution to overcome this problem. Plant growth regulators (PGRs) play important role in inducing callus cultures and the correct types and concentration of PGRs will enhance and optimize the growth of callus. This paper investigated the effects of different types of cytokinins (2,4-D and NAA) and auxins (BAP and kinetin) as PGRs at the range of 0.5, 1.0, 2.0, 3.0 and 4.0 mg/L, respectively supplemented with MS media, 3% (w/v) sugar, 500 mg/L malt extract and 0.25% (w/v) agar under the continuous dark condition. The cotyledons from the seeds of C. suhuiensis were excised as the explant and the growth of callus at each treatment was measured as g/L of fresh weight and percentage of callus induction. It was found that 1.0 mg/L 2, 4-D gave the highest growth of callus (0.15g and 100% callus percentage). After identifying the effective PGRs, Central Composite Design (CCD) from Design Expert software version 7.0 was used obtain the optimum concentration of cytokinin and auxin on C. suhuiensis callus culture. Result showed that the highest amount of callus induced was 0.218g when using 1.0 mg/L 2,4-D and 1.0 mg/L kinetin. Kulliyyah of Engineering, International Islamic University Malaysia 2016 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/51634/6/51634.pdf M. Fathil, Nur Alia and Mohamad Puad, Noor Illi and Amid, Azura and Azmi, Azlin Suhaida and Ibrahim, Rusli (2016) The effects of plant growth regulators to Citrus suhuiensis callus induction. In: 4th International Conference on Biotechnology Engineering 2016 (ICBioE 2016), 25th-27th July 2016, Kuala Lumpur.
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	SB Plant culture
spellingShingle	SB Plant culture M. Fathil, Nur Alia Mohamad Puad, Noor Illi Amid, Azura Azmi, Azlin Suhaida Ibrahim, Rusli The effects of plant growth regulators to Citrus suhuiensis callus induction
description	The diseases hampering Citrus suhuiensis (C. suhuiensis) or commonly known as ‘limau madu’ in East Asia has become a major problem to their cultivation and thus, plant cell culture techniques can be one of the solution to overcome this problem. Plant growth regulators (PGRs) play important role in inducing callus cultures and the correct types and concentration of PGRs will enhance and optimize the growth of callus. This paper investigated the effects of different types of cytokinins (2,4-D and NAA) and auxins (BAP and kinetin) as PGRs at the range of 0.5, 1.0, 2.0, 3.0 and 4.0 mg/L, respectively supplemented with MS media, 3% (w/v) sugar, 500 mg/L malt extract and 0.25% (w/v) agar under the continuous dark condition. The cotyledons from the seeds of C. suhuiensis were excised as the explant and the growth of callus at each treatment was measured as g/L of fresh weight and percentage of callus induction. It was found that 1.0 mg/L 2, 4-D gave the highest growth of callus (0.15g and 100% callus percentage). After identifying the effective PGRs, Central Composite Design (CCD) from Design Expert software version 7.0 was used obtain the optimum concentration of cytokinin and auxin on C. suhuiensis callus culture. Result showed that the highest amount of callus induced was 0.218g when using 1.0 mg/L 2,4-D and 1.0 mg/L kinetin.
format	Conference or Workshop Item
author	M. Fathil, Nur Alia Mohamad Puad, Noor Illi Amid, Azura Azmi, Azlin Suhaida Ibrahim, Rusli
author_facet	M. Fathil, Nur Alia Mohamad Puad, Noor Illi Amid, Azura Azmi, Azlin Suhaida Ibrahim, Rusli
author_sort	M. Fathil, Nur Alia
title	The effects of plant growth regulators to Citrus suhuiensis callus induction
title_short	The effects of plant growth regulators to Citrus suhuiensis callus induction
title_full	The effects of plant growth regulators to Citrus suhuiensis callus induction
title_fullStr	The effects of plant growth regulators to Citrus suhuiensis callus induction
title_full_unstemmed	The effects of plant growth regulators to Citrus suhuiensis callus induction
title_sort	effects of plant growth regulators to citrus suhuiensis callus induction
publisher	Kulliyyah of Engineering, International Islamic University Malaysia
publishDate	2016
url	http://irep.iium.edu.my/51634/ http://irep.iium.edu.my/51634/6/51634.pdf
first_indexed	2023-09-18T21:13:07Z
last_indexed	2023-09-18T21:13:07Z
_version_	1777411385213321216

The effects of plant growth regulators to Citrus suhuiensis callus induction

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