Piper sarmentosum (Daun kaduk) induced apoptosis via mitochondrial pathway in human lung adenocarcinoma cells, A549

Lung cancer has been reported as one of the top diseases in the world. Current cancer treatments like chemotherapy do not completely cure cancer and cause some side effects to the patient. Therefore, alternative strategies are being explored for cancer treatment and one of them is local herbs invest...

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Main Authors:	Sirajudeen, Azila, Mohd Ali, Aisyah Hanani, Abdul Ghani, Radiah
Format:	Conference or Workshop Item
Language:	English English
Published:	2016
Subjects:	RM214 Diet therapy. Diet and dietectics in disease
Online Access:	http://irep.iium.edu.my/51628/ http://irep.iium.edu.my/51628/ http://irep.iium.edu.my/51628/2/POSTER_MRS_2016.pdf http://irep.iium.edu.my/51628/6/51628_abstract.pdf

id	iium-51628
recordtype	eprints
spelling	iium-516282016-08-16T06:41:37Z http://irep.iium.edu.my/51628/ Piper sarmentosum (Daun kaduk) induced apoptosis via mitochondrial pathway in human lung adenocarcinoma cells, A549 Sirajudeen, Azila Mohd Ali, Aisyah Hanani Abdul Ghani, Radiah RM214 Diet therapy. Diet and dietectics in disease Lung cancer has been reported as one of the top diseases in the world. Current cancer treatments like chemotherapy do not completely cure cancer and cause some side effects to the patient. Therefore, alternative strategies are being explored for cancer treatment and one of them is local herbs investigation. In relation to this, Piper sarmentosum (daun kaduk) has received an attention due to its’ anti-cancer properties. It has been reported that both herbs induce anti-proliferative effects in human lung carcinoma cells, A549. Besides, one of the characteristics of anticancer herbs is the ability to induce cell death through apoptosis, which cause little side effects to the normal cells. In this study, the cell cycle profile and mechanism of cell death induced by daun kaduk was investigated using flow cytometer. The cell cycle profile changes were observed using Propidium iodide staining and it is found that P.sarmentosum arrested at G0/G1 phase in A549 cycles. Meanwhile, the Annexin V analysis revealed that P. sarmentosum showed significantly induction of apoptotic cells compared to untreated A549 cells. Furthermore, caspase analysis showed that P. sarmentosum induces apoptosis through mitochondrial pathway, by the activation of caspase 3 and caspase 9 significantly. Meanwhile, cytochrome c analysis reveals that P. sarmentosum induce a mitochondrial pathway of cell death mechanism by release of cytochrome c. Based on these preliminary findings, P. sarmentosum has a great potential as a dietary cancer treatment for lung cancer and may be used for lung cancer pharmacotherapy in clinical settings in future. 2016-08-11 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/51628/2/POSTER_MRS_2016.pdf application/pdf en http://irep.iium.edu.my/51628/6/51628_abstract.pdf Sirajudeen, Azila and Mohd Ali, Aisyah Hanani and Abdul Ghani, Radiah (2016) Piper sarmentosum (Daun kaduk) induced apoptosis via mitochondrial pathway in human lung adenocarcinoma cells, A549. In: Medical Research Symposium & Pacific Partnership in conjunction with Kuantan Research Day 2016, 3rd-5th August 2016, Kuantan, Pahang. (Unpublished) http://iiumedic.net/web/events/mrs2016/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RM214 Diet therapy. Diet and dietectics in disease
spellingShingle	RM214 Diet therapy. Diet and dietectics in disease Sirajudeen, Azila Mohd Ali, Aisyah Hanani Abdul Ghani, Radiah Piper sarmentosum (Daun kaduk) induced apoptosis via mitochondrial pathway in human lung adenocarcinoma cells, A549
description	Lung cancer has been reported as one of the top diseases in the world. Current cancer treatments like chemotherapy do not completely cure cancer and cause some side effects to the patient. Therefore, alternative strategies are being explored for cancer treatment and one of them is local herbs investigation. In relation to this, Piper sarmentosum (daun kaduk) has received an attention due to its’ anti-cancer properties. It has been reported that both herbs induce anti-proliferative effects in human lung carcinoma cells, A549. Besides, one of the characteristics of anticancer herbs is the ability to induce cell death through apoptosis, which cause little side effects to the normal cells. In this study, the cell cycle profile and mechanism of cell death induced by daun kaduk was investigated using flow cytometer. The cell cycle profile changes were observed using Propidium iodide staining and it is found that P.sarmentosum arrested at G0/G1 phase in A549 cycles. Meanwhile, the Annexin V analysis revealed that P. sarmentosum showed significantly induction of apoptotic cells compared to untreated A549 cells. Furthermore, caspase analysis showed that P. sarmentosum induces apoptosis through mitochondrial pathway, by the activation of caspase 3 and caspase 9 significantly. Meanwhile, cytochrome c analysis reveals that P. sarmentosum induce a mitochondrial pathway of cell death mechanism by release of cytochrome c. Based on these preliminary findings, P. sarmentosum has a great potential as a dietary cancer treatment for lung cancer and may be used for lung cancer pharmacotherapy in clinical settings in future.
format	Conference or Workshop Item
author	Sirajudeen, Azila Mohd Ali, Aisyah Hanani Abdul Ghani, Radiah
author_facet	Sirajudeen, Azila Mohd Ali, Aisyah Hanani Abdul Ghani, Radiah
author_sort	Sirajudeen, Azila
title	Piper sarmentosum (Daun kaduk) induced apoptosis via mitochondrial pathway in human lung adenocarcinoma cells, A549
title_short	Piper sarmentosum (Daun kaduk) induced apoptosis via mitochondrial pathway in human lung adenocarcinoma cells, A549
title_full	Piper sarmentosum (Daun kaduk) induced apoptosis via mitochondrial pathway in human lung adenocarcinoma cells, A549
title_fullStr	Piper sarmentosum (Daun kaduk) induced apoptosis via mitochondrial pathway in human lung adenocarcinoma cells, A549
title_full_unstemmed	Piper sarmentosum (Daun kaduk) induced apoptosis via mitochondrial pathway in human lung adenocarcinoma cells, A549
title_sort	piper sarmentosum (daun kaduk) induced apoptosis via mitochondrial pathway in human lung adenocarcinoma cells, a549
publishDate	2016
url	http://irep.iium.edu.my/51628/ http://irep.iium.edu.my/51628/ http://irep.iium.edu.my/51628/2/POSTER_MRS_2016.pdf http://irep.iium.edu.my/51628/6/51628_abstract.pdf
first_indexed	2023-09-18T21:13:07Z
last_indexed	2023-09-18T21:13:07Z
_version_	1777411384769773568

Piper sarmentosum (Daun kaduk) induced apoptosis via mitochondrial pathway in human lung adenocarcinoma cells, A549

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