A novel aqueous micellar two phase system composed of surfactant and mannitol for purification of polygalacturonase enzyme from durio zibethinus murry and recycling phase components
A novel aqueous micellar two-phase system composed of surfactant and mannitol was employed for the first time to purify polygalacturonase from Durio zibethinus Murray. The influences of different parameters such as type of surfactants, composition of surfactant/mannitol, pH and crude load on polygal...
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| Format: | Article |
| Language: | English English |
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Taylor & Francis Group, LLC
2016
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| Online Access: | http://irep.iium.edu.my/51508/ http://irep.iium.edu.my/51508/ http://irep.iium.edu.my/51508/ http://irep.iium.edu.my/51508/5/51508_A%20Novel%20Aqueous%20Micellar_SCOPUS.pdf http://irep.iium.edu.my/51508/16/51508_A%20novel%20aqueous%20micellar%20two-phase%20system.pdf |
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iium-51508 |
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eprints |
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iium-515082018-06-21T02:47:19Z http://irep.iium.edu.my/51508/ A novel aqueous micellar two phase system composed of surfactant and mannitol for purification of polygalacturonase enzyme from durio zibethinus murry and recycling phase components Amid, Mehrnoush Asmadi, Farhana Azmira Hussina, Muhaini Manap, Mohd Yazid Sarker, Md. Zaidul Islam Hean, Chong Gun Q Science (General) T Technology (General) A novel aqueous micellar two-phase system composed of surfactant and mannitol was employed for the first time to purify polygalacturonase from Durio zibethinus Murray. The influences of different parameters such as type of surfactants, composition of surfactant/mannitol, pH and crude load on polygalacturonase partitioning were investigated. The results indicated that the polygalacturonase was recovered in 25% Tween 80 and 22% mannitol, at 68.2% of Tie line length, crude load of 30% at pH 5.0. The enzyme was successfully recovered with a high purification factor of 16.1 and yield of 97.3% while phase components were also recovered and recycled above 95%. Taylor & Francis Group, LLC 2016-04-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/51508/5/51508_A%20Novel%20Aqueous%20Micellar_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/51508/16/51508_A%20novel%20aqueous%20micellar%20two-phase%20system.pdf Amid, Mehrnoush and Asmadi, Farhana Azmira and Hussina, Muhaini and Manap, Mohd Yazid and Sarker, Md. Zaidul Islam and Hean, Chong Gun (2016) A novel aqueous micellar two phase system composed of surfactant and mannitol for purification of polygalacturonase enzyme from durio zibethinus murry and recycling phase components. Separation Science and Technology, 51 (6). pp. 968-975. ISSN 0149-6395 E-ISSN 1520-5754 http://www.tandfonline.com/doi/abs/10.1080/01496395.2016.1142562 10.1080/01496395.2016.1142562 |
| repository_type |
Digital Repository |
| institution_category |
Local University |
| institution |
International Islamic University Malaysia |
| building |
IIUM Repository |
| collection |
Online Access |
| language |
English English |
| topic |
Q Science (General) T Technology (General) |
| spellingShingle |
Q Science (General) T Technology (General) Amid, Mehrnoush Asmadi, Farhana Azmira Hussina, Muhaini Manap, Mohd Yazid Sarker, Md. Zaidul Islam Hean, Chong Gun A novel aqueous micellar two phase system composed of surfactant and mannitol for purification of polygalacturonase enzyme from durio zibethinus murry and recycling phase components |
| description |
A novel aqueous micellar two-phase system composed of surfactant and mannitol was employed for the first time to purify polygalacturonase from Durio zibethinus Murray. The influences of different parameters such as type of surfactants, composition of surfactant/mannitol, pH and crude load on polygalacturonase partitioning were investigated. The results indicated that the polygalacturonase was recovered in 25% Tween 80 and 22% mannitol, at 68.2% of Tie line length, crude load of 30% at pH 5.0. The enzyme was successfully recovered with a high purification factor of 16.1 and yield of 97.3% while phase components were also recovered and recycled above 95%. |
| format |
Article |
| author |
Amid, Mehrnoush Asmadi, Farhana Azmira Hussina, Muhaini Manap, Mohd Yazid Sarker, Md. Zaidul Islam Hean, Chong Gun |
| author_facet |
Amid, Mehrnoush Asmadi, Farhana Azmira Hussina, Muhaini Manap, Mohd Yazid Sarker, Md. Zaidul Islam Hean, Chong Gun |
| author_sort |
Amid, Mehrnoush |
| title |
A novel aqueous micellar two phase system composed of surfactant and mannitol for purification of polygalacturonase enzyme from durio zibethinus murry and recycling phase components |
| title_short |
A novel aqueous micellar two phase system composed of surfactant and mannitol for purification of polygalacturonase enzyme from durio zibethinus murry and recycling phase components |
| title_full |
A novel aqueous micellar two phase system composed of surfactant and mannitol for purification of polygalacturonase enzyme from durio zibethinus murry and recycling phase components |
| title_fullStr |
A novel aqueous micellar two phase system composed of surfactant and mannitol for purification of polygalacturonase enzyme from durio zibethinus murry and recycling phase components |
| title_full_unstemmed |
A novel aqueous micellar two phase system composed of surfactant and mannitol for purification of polygalacturonase enzyme from durio zibethinus murry and recycling phase components |
| title_sort |
novel aqueous micellar two phase system composed of surfactant and mannitol for purification of polygalacturonase enzyme from durio zibethinus murry and recycling phase components |
| publisher |
Taylor & Francis Group, LLC |
| publishDate |
2016 |
| url |
http://irep.iium.edu.my/51508/ http://irep.iium.edu.my/51508/ http://irep.iium.edu.my/51508/ http://irep.iium.edu.my/51508/5/51508_A%20Novel%20Aqueous%20Micellar_SCOPUS.pdf http://irep.iium.edu.my/51508/16/51508_A%20novel%20aqueous%20micellar%20two-phase%20system.pdf |
| first_indexed |
2023-09-18T21:12:55Z |
| last_indexed |
2023-09-18T21:12:55Z |
| _version_ |
1777411372445859840 |