A novel aqueous micellar two phase system composed of surfactant and mannitol for purification of polygalacturonase enzyme from durio zibethinus murry and recycling phase components

A novel aqueous micellar two-phase system composed of surfactant and mannitol was employed for the first time to purify polygalacturonase from Durio zibethinus Murray. The influences of different parameters such as type of surfactants, composition of surfactant/mannitol, pH and crude load on polygal...

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Main Authors: Amid, Mehrnoush, Asmadi, Farhana Azmira, Hussina, Muhaini, Manap, Mohd Yazid, Sarker, Md. Zaidul Islam, Hean, Chong Gun
Format: Article
Language:English
English
Published: Taylor & Francis Group, LLC 2016
Subjects:
Online Access:http://irep.iium.edu.my/51508/
http://irep.iium.edu.my/51508/
http://irep.iium.edu.my/51508/
http://irep.iium.edu.my/51508/5/51508_A%20Novel%20Aqueous%20Micellar_SCOPUS.pdf
http://irep.iium.edu.my/51508/16/51508_A%20novel%20aqueous%20micellar%20two-phase%20system.pdf
id iium-51508
recordtype eprints
spelling iium-515082018-06-21T02:47:19Z http://irep.iium.edu.my/51508/ A novel aqueous micellar two phase system composed of surfactant and mannitol for purification of polygalacturonase enzyme from durio zibethinus murry and recycling phase components Amid, Mehrnoush Asmadi, Farhana Azmira Hussina, Muhaini Manap, Mohd Yazid Sarker, Md. Zaidul Islam Hean, Chong Gun Q Science (General) T Technology (General) A novel aqueous micellar two-phase system composed of surfactant and mannitol was employed for the first time to purify polygalacturonase from Durio zibethinus Murray. The influences of different parameters such as type of surfactants, composition of surfactant/mannitol, pH and crude load on polygalacturonase partitioning were investigated. The results indicated that the polygalacturonase was recovered in 25% Tween 80 and 22% mannitol, at 68.2% of Tie line length, crude load of 30% at pH 5.0. The enzyme was successfully recovered with a high purification factor of 16.1 and yield of 97.3% while phase components were also recovered and recycled above 95%. Taylor & Francis Group, LLC 2016-04-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/51508/5/51508_A%20Novel%20Aqueous%20Micellar_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/51508/16/51508_A%20novel%20aqueous%20micellar%20two-phase%20system.pdf Amid, Mehrnoush and Asmadi, Farhana Azmira and Hussina, Muhaini and Manap, Mohd Yazid and Sarker, Md. Zaidul Islam and Hean, Chong Gun (2016) A novel aqueous micellar two phase system composed of surfactant and mannitol for purification of polygalacturonase enzyme from durio zibethinus murry and recycling phase components. Separation Science and Technology, 51 (6). pp. 968-975. ISSN 0149-6395 E-ISSN 1520-5754 http://www.tandfonline.com/doi/abs/10.1080/01496395.2016.1142562 10.1080/01496395.2016.1142562
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
English
topic Q Science (General)
T Technology (General)
spellingShingle Q Science (General)
T Technology (General)
Amid, Mehrnoush
Asmadi, Farhana Azmira
Hussina, Muhaini
Manap, Mohd Yazid
Sarker, Md. Zaidul Islam
Hean, Chong Gun
A novel aqueous micellar two phase system composed of surfactant and mannitol for purification of polygalacturonase enzyme from durio zibethinus murry and recycling phase components
description A novel aqueous micellar two-phase system composed of surfactant and mannitol was employed for the first time to purify polygalacturonase from Durio zibethinus Murray. The influences of different parameters such as type of surfactants, composition of surfactant/mannitol, pH and crude load on polygalacturonase partitioning were investigated. The results indicated that the polygalacturonase was recovered in 25% Tween 80 and 22% mannitol, at 68.2% of Tie line length, crude load of 30% at pH 5.0. The enzyme was successfully recovered with a high purification factor of 16.1 and yield of 97.3% while phase components were also recovered and recycled above 95%.
format Article
author Amid, Mehrnoush
Asmadi, Farhana Azmira
Hussina, Muhaini
Manap, Mohd Yazid
Sarker, Md. Zaidul Islam
Hean, Chong Gun
author_facet Amid, Mehrnoush
Asmadi, Farhana Azmira
Hussina, Muhaini
Manap, Mohd Yazid
Sarker, Md. Zaidul Islam
Hean, Chong Gun
author_sort Amid, Mehrnoush
title A novel aqueous micellar two phase system composed of surfactant and mannitol for purification of polygalacturonase enzyme from durio zibethinus murry and recycling phase components
title_short A novel aqueous micellar two phase system composed of surfactant and mannitol for purification of polygalacturonase enzyme from durio zibethinus murry and recycling phase components
title_full A novel aqueous micellar two phase system composed of surfactant and mannitol for purification of polygalacturonase enzyme from durio zibethinus murry and recycling phase components
title_fullStr A novel aqueous micellar two phase system composed of surfactant and mannitol for purification of polygalacturonase enzyme from durio zibethinus murry and recycling phase components
title_full_unstemmed A novel aqueous micellar two phase system composed of surfactant and mannitol for purification of polygalacturonase enzyme from durio zibethinus murry and recycling phase components
title_sort novel aqueous micellar two phase system composed of surfactant and mannitol for purification of polygalacturonase enzyme from durio zibethinus murry and recycling phase components
publisher Taylor & Francis Group, LLC
publishDate 2016
url http://irep.iium.edu.my/51508/
http://irep.iium.edu.my/51508/
http://irep.iium.edu.my/51508/
http://irep.iium.edu.my/51508/5/51508_A%20Novel%20Aqueous%20Micellar_SCOPUS.pdf
http://irep.iium.edu.my/51508/16/51508_A%20novel%20aqueous%20micellar%20two-phase%20system.pdf
first_indexed 2023-09-18T21:12:55Z
last_indexed 2023-09-18T21:12:55Z
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