A comprehensive study of chronic diabetes complications in streptozotocin-induced diabetic rat

Objective: The purpose of this study was to provide a reference of chronic diabetes complications by investigating the prolonged hyperglycemia effects on hematological, biochemical and histopathological changes (liver, kidney, spleen, cardiac muscle, adrenal gland, and endocrine pancreas) in diabe...

Full description

Bibliographic Details
Main Authors:	Al-Mahmood, Sinan, Abdul Razak, Tariq, Che Abdullah, Shahrin T, NA, Nik Nur Fatnoon, Mohamed, Abdul Hadi, Al-Ani, Imad Matloub Dally
Format:	Article
Language:	English English
Published:	Directorate of Research and Public Services Universitas Indonesia 2016
Subjects:	R Medicine (General) RB Pathology
Online Access:	http://irep.iium.edu.my/51478/ http://irep.iium.edu.my/51478/ http://irep.iium.edu.my/51478/ http://irep.iium.edu.my/51478/1/5889-11652-1-PB.pdf http://irep.iium.edu.my/51478/4/51478_A%20comprehensive%20study%20of%20chronic%20diabetes_wos.pdf

id	iium-51478
recordtype	eprints
spelling	iium-514782017-10-09T01:23:34Z http://irep.iium.edu.my/51478/ A comprehensive study of chronic diabetes complications in streptozotocin-induced diabetic rat Al-Mahmood, Sinan Abdul Razak, Tariq Che Abdullah, Shahrin T NA, Nik Nur Fatnoon Mohamed, Abdul Hadi Al-Ani, Imad Matloub Dally R Medicine (General) RB Pathology Objective: The purpose of this study was to provide a reference of chronic diabetes complications by investigating the prolonged hyperglycemia effects on hematological, biochemical and histopathological changes (liver, kidney, spleen, cardiac muscle, adrenal gland, and endocrine pancreas) in diabetic rats induced by streptozotocin. Methods: Ten adult female Sprague-Dawley of uniform age were divided into two Groups. Group 1 was made diabetic by single intraperitoneal injection of streptozotocin (60 mg/kg/bw) whereas Group 2 served as control. After six months, the rats were anesthetized using pentobarbital. Cardiac puncture was performed to get 3 ml of the blood sample; following 12 hours of an overnight fast. Serum chemistry test and complete blood analysis for lipid profile and blood glucose test; liver and renal functions were performed. Tissue specimens of liver, kidney, spleen, cardiac muscle, adrenal gland, and endocrine pancreas were fixed in 10% formal saline and processed for histological study. Results: There were severe histopathological changes in the affected organs; and the presence of a significant abnormality of lipid profile, liver, and renal functions. Conclusions: The presence of histopathological changes with abnormal biochemical changes is related to the chronic absence of insulin production in the destroyed β –cells which reflect the diabetic complications in a human being. Directorate of Research and Public Services Universitas Indonesia 2016-08 Article PeerReviewed application/pdf en http://irep.iium.edu.my/51478/1/5889-11652-1-PB.pdf application/pdf en http://irep.iium.edu.my/51478/4/51478_A%20comprehensive%20study%20of%20chronic%20diabetes_wos.pdf Al-Mahmood, Sinan and Abdul Razak, Tariq and Che Abdullah, Shahrin T and NA, Nik Nur Fatnoon and Mohamed, Abdul Hadi and Al-Ani, Imad Matloub Dally (2016) A comprehensive study of chronic diabetes complications in streptozotocin-induced diabetic rat. Makara Journal of Health Research, 20 (2). pp. 48-56. ISSN 2356-3664 E-ISSN 2356-3656 http://journal.ui.ac.id/index.php/health/article/view/5889/3688 10.7454/msk.v20i2.5889
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	R Medicine (General) RB Pathology
spellingShingle	R Medicine (General) RB Pathology Al-Mahmood, Sinan Abdul Razak, Tariq Che Abdullah, Shahrin T NA, Nik Nur Fatnoon Mohamed, Abdul Hadi Al-Ani, Imad Matloub Dally A comprehensive study of chronic diabetes complications in streptozotocin-induced diabetic rat
description	Objective: The purpose of this study was to provide a reference of chronic diabetes complications by investigating the prolonged hyperglycemia effects on hematological, biochemical and histopathological changes (liver, kidney, spleen, cardiac muscle, adrenal gland, and endocrine pancreas) in diabetic rats induced by streptozotocin. Methods: Ten adult female Sprague-Dawley of uniform age were divided into two Groups. Group 1 was made diabetic by single intraperitoneal injection of streptozotocin (60 mg/kg/bw) whereas Group 2 served as control. After six months, the rats were anesthetized using pentobarbital. Cardiac puncture was performed to get 3 ml of the blood sample; following 12 hours of an overnight fast. Serum chemistry test and complete blood analysis for lipid profile and blood glucose test; liver and renal functions were performed. Tissue specimens of liver, kidney, spleen, cardiac muscle, adrenal gland, and endocrine pancreas were fixed in 10% formal saline and processed for histological study. Results: There were severe histopathological changes in the affected organs; and the presence of a significant abnormality of lipid profile, liver, and renal functions. Conclusions: The presence of histopathological changes with abnormal biochemical changes is related to the chronic absence of insulin production in the destroyed β –cells which reflect the diabetic complications in a human being.
format	Article
author	Al-Mahmood, Sinan Abdul Razak, Tariq Che Abdullah, Shahrin T NA, Nik Nur Fatnoon Mohamed, Abdul Hadi Al-Ani, Imad Matloub Dally
author_facet	Al-Mahmood, Sinan Abdul Razak, Tariq Che Abdullah, Shahrin T NA, Nik Nur Fatnoon Mohamed, Abdul Hadi Al-Ani, Imad Matloub Dally
author_sort	Al-Mahmood, Sinan
title	A comprehensive study of chronic diabetes complications in streptozotocin-induced diabetic rat
title_short	A comprehensive study of chronic diabetes complications in streptozotocin-induced diabetic rat
title_full	A comprehensive study of chronic diabetes complications in streptozotocin-induced diabetic rat
title_fullStr	A comprehensive study of chronic diabetes complications in streptozotocin-induced diabetic rat
title_full_unstemmed	A comprehensive study of chronic diabetes complications in streptozotocin-induced diabetic rat
title_sort	comprehensive study of chronic diabetes complications in streptozotocin-induced diabetic rat
publisher	Directorate of Research and Public Services Universitas Indonesia
publishDate	2016
url	http://irep.iium.edu.my/51478/ http://irep.iium.edu.my/51478/ http://irep.iium.edu.my/51478/ http://irep.iium.edu.my/51478/1/5889-11652-1-PB.pdf http://irep.iium.edu.my/51478/4/51478_A%20comprehensive%20study%20of%20chronic%20diabetes_wos.pdf
first_indexed	2023-09-18T21:12:52Z
last_indexed	2023-09-18T21:12:52Z
_version_	1777411369226731520

A comprehensive study of chronic diabetes complications in streptozotocin-induced diabetic rat

Similar Items