Identification of aldolase C, actin-related protein 2 homolog, and enolase 1 as potential biomarkers for electrically stunned chickens

Halal slaughtered chicken is a major concern among Muslims. Currently,stunning methods has been adopted worldwide aiming at maximizing production. However, stunning treatment which is not in compliance with the Islamic teaching will end up with unlawful chickens for Muslim consumption. In this study...

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Main Authors:	Abu Samah, Norshahida, Amid, Azura, Yusof, Faridah
Other Authors:	Ab. Manan, Siti Khadijah
Format:	Book Chapter
Language:	English
Published:	Springer Singapore 2016
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/51398/ http://irep.iium.edu.my/51398/ http://irep.iium.edu.my/51398/1/51398_Identification%20of%20aldolase%20C%2C%20actin-related%20protein%202%20homolog%2C%20and%20enolase%201%20as%20potential%20biomarkers%20for%20electrically%20stunned%20chickens.pdf

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spelling	iium-513982016-10-24T04:28:02Z http://irep.iium.edu.my/51398/ Identification of aldolase C, actin-related protein 2 homolog, and enolase 1 as potential biomarkers for electrically stunned chickens Abu Samah, Norshahida Amid, Azura Yusof, Faridah TP248.13 Biotechnology Halal slaughtered chicken is a major concern among Muslims. Currently,stunning methods has been adopted worldwide aiming at maximizing production. However, stunning treatment which is not in compliance with the Islamic teaching will end up with unlawful chickens for Muslim consumption. In this study, proteomics methods have been used to identify potential biomarkers for the effects of electrical stimulation on the protein profiles of chicken’s brain. The protein profiles were constructed in order to detect any differences in the protein expression and modifications. The electrical stimulation was conducted with different current and voltage. The different voltages studied were 10, 40, and 70 V while the values for current studied were 0.25, 0.5, and 0.75 A. The profiles from these electrically stimulated chicken were compared to the 0 A, 0 V non-stunned sample. After the proteomics analyses using 2D Platinum ImageMaster 6.0 and matrix-assisted laser desorption/ionization with time-of-flight mass spectrometry (MALDI-TOF) identification, aldolase C, actin-related protein 2 homolog (ARP2), and enolase 1 were identified to be positively present in the brain sample of electrically stimulated samples. The over expression of the proteins were further confirmed at the transcriptional level by real-time PCR. Results from MALDI-TOF and real-time PCRwere in agreement. Therefore, this identification of biomarkers for electrically stimulated chicken’s brain provides a novel approach on differentiating the stunned chickens from the non-stunned ones. Springer Singapore Ab. Manan, Siti Khadijah Abd Rahman, Fadilah Sahri, Mardhiyyah 2016 Book Chapter PeerReviewed application/pdf en http://irep.iium.edu.my/51398/1/51398_Identification%20of%20aldolase%20C%2C%20actin-related%20protein%202%20homolog%2C%20and%20enolase%201%20as%20potential%20biomarkers%20for%20electrically%20stunned%20chickens.pdf Abu Samah, Norshahida and Amid, Azura and Yusof, Faridah (2016) Identification of aldolase C, actin-related protein 2 homolog, and enolase 1 as potential biomarkers for electrically stunned chickens. In: Contemporary issues and development in the global Halal industry: selected papers from the International Halal Conference 2014. Springer Singapore, Singapore, pp. 519-530. ISBN 978-981-10-1450-5 http://link.springer.com/chapter/10.1007%2F978-981-10-1452-9_47
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Abu Samah, Norshahida Amid, Azura Yusof, Faridah Identification of aldolase C, actin-related protein 2 homolog, and enolase 1 as potential biomarkers for electrically stunned chickens
description	Halal slaughtered chicken is a major concern among Muslims. Currently,stunning methods has been adopted worldwide aiming at maximizing production. However, stunning treatment which is not in compliance with the Islamic teaching will end up with unlawful chickens for Muslim consumption. In this study, proteomics methods have been used to identify potential biomarkers for the effects of electrical stimulation on the protein profiles of chicken’s brain. The protein profiles were constructed in order to detect any differences in the protein expression and modifications. The electrical stimulation was conducted with different current and voltage. The different voltages studied were 10, 40, and 70 V while the values for current studied were 0.25, 0.5, and 0.75 A. The profiles from these electrically stimulated chicken were compared to the 0 A, 0 V non-stunned sample. After the proteomics analyses using 2D Platinum ImageMaster 6.0 and matrix-assisted laser desorption/ionization with time-of-flight mass spectrometry (MALDI-TOF) identification, aldolase C, actin-related protein 2 homolog (ARP2), and enolase 1 were identified to be positively present in the brain sample of electrically stimulated samples. The over expression of the proteins were further confirmed at the transcriptional level by real-time PCR. Results from MALDI-TOF and real-time PCRwere in agreement. Therefore, this identification of biomarkers for electrically stimulated chicken’s brain provides a novel approach on differentiating the stunned chickens from the non-stunned ones.
author2	Ab. Manan, Siti Khadijah
author_facet	Ab. Manan, Siti Khadijah Abu Samah, Norshahida Amid, Azura Yusof, Faridah
format	Book Chapter
author	Abu Samah, Norshahida Amid, Azura Yusof, Faridah
author_sort	Abu Samah, Norshahida
title	Identification of aldolase C, actin-related protein 2 homolog, and enolase 1 as potential biomarkers for electrically stunned chickens
title_short	Identification of aldolase C, actin-related protein 2 homolog, and enolase 1 as potential biomarkers for electrically stunned chickens
title_full	Identification of aldolase C, actin-related protein 2 homolog, and enolase 1 as potential biomarkers for electrically stunned chickens
title_fullStr	Identification of aldolase C, actin-related protein 2 homolog, and enolase 1 as potential biomarkers for electrically stunned chickens
title_full_unstemmed	Identification of aldolase C, actin-related protein 2 homolog, and enolase 1 as potential biomarkers for electrically stunned chickens
title_sort	identification of aldolase c, actin-related protein 2 homolog, and enolase 1 as potential biomarkers for electrically stunned chickens
publisher	Springer Singapore
publishDate	2016
url	http://irep.iium.edu.my/51398/ http://irep.iium.edu.my/51398/ http://irep.iium.edu.my/51398/1/51398_Identification%20of%20aldolase%20C%2C%20actin-related%20protein%202%20homolog%2C%20and%20enolase%201%20as%20potential%20biomarkers%20for%20electrically%20stunned%20chickens.pdf
first_indexed	2023-09-18T21:12:45Z
last_indexed	2023-09-18T21:12:45Z
_version_	1777411362394210304

Identification of aldolase C, actin-related protein 2 homolog, and enolase 1 as potential biomarkers for electrically stunned chickens

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