Phenolics and flavonoids content determination and in-vitro antioxidant evaluation for radical scavenging activity of extracts from Dialium indum var. indum L. fruits

Phenolics and flavonoids content determination and in-vitro antioxidant evaluation for radical scavenging activity of extracts from Dialium indum var. indum L. fruits

Phenolic compounds are the largest group of phytochemicals, which have been studied to account for most plants antioxidant activity while flavonoids are the largest group of plants phenolics. High phenolics, flavonoids, saponins, and tannins content have been linked to the high antioxidant activity...

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Main Authors: Osman, Muhamad Faris, Mohd Hassan, Norazian
Format: Conference or Workshop Item
Language:English
English
Published: 2016
Subjects:
QD Chemistry
RS Pharmacy and materia medica
Online Access:http://irep.iium.edu.my/51280/
http://irep.iium.edu.my/51280/1/EXTENDED_ABSTRACTS-FARIS.pdf
http://irep.iium.edu.my/51280/7/51280.pdf
id iium-51280
recordtype eprints
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
English
topic QD Chemistry
RS Pharmacy and materia medica
spellingShingle QD Chemistry
RS Pharmacy and materia medica
Osman, Muhamad Faris
Mohd Hassan, Norazian
Phenolics and flavonoids content determination and in-vitro antioxidant evaluation for radical scavenging activity of extracts from Dialium indum var. indum L. fruits
description Phenolic compounds are the largest group of phytochemicals, which have been studied to account for most plants antioxidant activity while flavonoids are the largest group of plants phenolics. High phenolics, flavonoids, saponins, and tannins content have been linked to the high antioxidant activity of plant extracts. Intake of plants with high phenolics content is linked with reduced risk for chronic disease. Dialium indum or black velvet tamarind is known by Malaysians and Indonesians as keranji which is sold at local markets as sweets. This study was conducted to investigate the total phenolics and flavonoids content and in-vitro DPPH radical scavenging activity of D. indum var. indum L. fruits. Samples (mesocarp, pericarp, and seed of fruits) were extracted by using cold maceration with distilled water heated to 40 °C for 1 hour and then frozen at -80 °C. All samples were then freeze-dried. For direct methanolic extract, all samples were cold macerated in methanol, filtered, then dried by using rotary evaporator at 40 °C. For consecutive organic solvents extraction, the same procedure for direct methanol extraction was used but all samples were cold macerated in n- hexane, dichloromethane, and methanol consecutively. The total phenolic (TPC) was determined using gallic acid standard curve while total flavonoid content (TFC) was determined using quercetin standard curve. Results were expressed as mg quercetin equivalence (QE) per gram of extract ± SEM. DPPH radical scavenging activity was employed to determine the percentage of inhibition of DPPH radical and IC50 were calculated. The results were compared with the standard ascorbic acid. Data were statistically analyzed using Analysis Toolpak, Microsoft Excel 2013. One-way analysis of variance (ANOVA) and Pearson correlation coefficient was determined and the significant difference was set at p < 0.05. Fractionated methanolic seed extract of the fruits contained highest amount of phenolics (93.51 mg GAE/g dry extract), followed by direct methanolic seed extract (59.95 mg GAE/g dry extract), and fractionated dichloromethane pericarp extract (43.26 mg GAE/g dry extract). Total flavonoids contents of the extracts showed that the fractionated dichloromethane pericarp extract had the highest amount of flavonoids (9.18 mg QE/g dry extract). A moderate positive correlation (r = 0.46) was obtained between total phenolics and total flavonoids content for all extracts tested. IC50 of fractionated dichloromethane pericarp extract was found to be 71.28 μg/mL while the standard ascorbic acid had IC50 of 1.44 μg/mL. There was significant difference (p < 0.05) in percent of DPPH radical inhibition at highest concentration tested (1000 μg/mL) between fractionated dichloromethane pericarp extract and the standard ascorbic acid. This study showed that the fractionated dichloromethane extract of D. indum var. indum L. fruit pericarp had positive results for flavonoids and DPPH radical scavenging activity. The results indicated that the flavonoids with antioxidant activity were semipolar in nature. This study acknowledged the in-vitro antioxidant properties of D. indum fruit pericarp; thus, indicated that this underutilized fruit has potential utilization in future natural products.
format Conference or Workshop Item
author Osman, Muhamad Faris
Mohd Hassan, Norazian
author_facet Osman, Muhamad Faris
Mohd Hassan, Norazian
author_sort Osman, Muhamad Faris
title Phenolics and flavonoids content determination and in-vitro antioxidant evaluation for radical scavenging activity of extracts from Dialium indum var. indum L. fruits
title_short Phenolics and flavonoids content determination and in-vitro antioxidant evaluation for radical scavenging activity of extracts from Dialium indum var. indum L. fruits
title_full Phenolics and flavonoids content determination and in-vitro antioxidant evaluation for radical scavenging activity of extracts from Dialium indum var. indum L. fruits
title_fullStr Phenolics and flavonoids content determination and in-vitro antioxidant evaluation for radical scavenging activity of extracts from Dialium indum var. indum L. fruits
title_full_unstemmed Phenolics and flavonoids content determination and in-vitro antioxidant evaluation for radical scavenging activity of extracts from Dialium indum var. indum L. fruits
title_sort phenolics and flavonoids content determination and in-vitro antioxidant evaluation for radical scavenging activity of extracts from dialium indum var. indum l. fruits
publishDate 2016
url http://irep.iium.edu.my/51280/
http://irep.iium.edu.my/51280/1/EXTENDED_ABSTRACTS-FARIS.pdf
http://irep.iium.edu.my/51280/7/51280.pdf
first_indexed 2023-09-18T21:12:35Z
last_indexed 2023-09-18T21:12:35Z
_version_ 1777411351589683200
spelling iium-512802016-07-26T03:46:12Z http://irep.iium.edu.my/51280/ Phenolics and flavonoids content determination and in-vitro antioxidant evaluation for radical scavenging activity of extracts from Dialium indum var. indum L. fruits Osman, Muhamad Faris Mohd Hassan, Norazian QD Chemistry RS Pharmacy and materia medica Phenolic compounds are the largest group of phytochemicals, which have been studied to account for most plants antioxidant activity while flavonoids are the largest group of plants phenolics. High phenolics, flavonoids, saponins, and tannins content have been linked to the high antioxidant activity of plant extracts. Intake of plants with high phenolics content is linked with reduced risk for chronic disease. Dialium indum or black velvet tamarind is known by Malaysians and Indonesians as keranji which is sold at local markets as sweets. This study was conducted to investigate the total phenolics and flavonoids content and in-vitro DPPH radical scavenging activity of D. indum var. indum L. fruits. Samples (mesocarp, pericarp, and seed of fruits) were extracted by using cold maceration with distilled water heated to 40 °C for 1 hour and then frozen at -80 °C. All samples were then freeze-dried. For direct methanolic extract, all samples were cold macerated in methanol, filtered, then dried by using rotary evaporator at 40 °C. For consecutive organic solvents extraction, the same procedure for direct methanol extraction was used but all samples were cold macerated in n- hexane, dichloromethane, and methanol consecutively. The total phenolic (TPC) was determined using gallic acid standard curve while total flavonoid content (TFC) was determined using quercetin standard curve. Results were expressed as mg quercetin equivalence (QE) per gram of extract ± SEM. DPPH radical scavenging activity was employed to determine the percentage of inhibition of DPPH radical and IC50 were calculated. The results were compared with the standard ascorbic acid. Data were statistically analyzed using Analysis Toolpak, Microsoft Excel 2013. One-way analysis of variance (ANOVA) and Pearson correlation coefficient was determined and the significant difference was set at p < 0.05. Fractionated methanolic seed extract of the fruits contained highest amount of phenolics (93.51 mg GAE/g dry extract), followed by direct methanolic seed extract (59.95 mg GAE/g dry extract), and fractionated dichloromethane pericarp extract (43.26 mg GAE/g dry extract). Total flavonoids contents of the extracts showed that the fractionated dichloromethane pericarp extract had the highest amount of flavonoids (9.18 mg QE/g dry extract). A moderate positive correlation (r = 0.46) was obtained between total phenolics and total flavonoids content for all extracts tested. IC50 of fractionated dichloromethane pericarp extract was found to be 71.28 μg/mL while the standard ascorbic acid had IC50 of 1.44 μg/mL. There was significant difference (p < 0.05) in percent of DPPH radical inhibition at highest concentration tested (1000 μg/mL) between fractionated dichloromethane pericarp extract and the standard ascorbic acid. This study showed that the fractionated dichloromethane extract of D. indum var. indum L. fruit pericarp had positive results for flavonoids and DPPH radical scavenging activity. The results indicated that the flavonoids with antioxidant activity were semipolar in nature. This study acknowledged the in-vitro antioxidant properties of D. indum fruit pericarp; thus, indicated that this underutilized fruit has potential utilization in future natural products. 2016 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/51280/1/EXTENDED_ABSTRACTS-FARIS.pdf application/pdf en http://irep.iium.edu.my/51280/7/51280.pdf Osman, Muhamad Faris and Mohd Hassan, Norazian (2016) Phenolics and flavonoids content determination and in-vitro antioxidant evaluation for radical scavenging activity of extracts from Dialium indum var. indum L. fruits. In: Research Symposium 2016: Learn, Discover, Innovate, 12-13 April 2016, Kuala Lumpur, Malaysia. (Unpublished)

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