Cocoa pod husk, a new source of hydrolase enzymes for preparation of cross‑linked enzyme aggregate

Cocoa pod husk (CPH) is a by-product of cocoa production obtained after removing the beans from the fruit. The analysis of CPH has shown that it contains high amounts of protein. This study is aimed to utilize this protein source in hydrolase enzyme production. In this study, seven hydrolase enzym...

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Main Authors:	Yusof, Faridah, Khanahmadi, Soofia, Amid, Azura, Mahmod, Safa Senan
Format:	Article
Language:	English English
Published:	Springerplus 2016
Subjects:	T Technology (General) TP155 Chemical engineering
Online Access:	http://irep.iium.edu.my/51185/ http://irep.iium.edu.my/51185/ http://irep.iium.edu.my/51185/ http://irep.iium.edu.my/51185/1/51185_Cocoa%20pod%20husk.pdf http://irep.iium.edu.my/51185/2/51185_Cocoa%20pod%20husk_WOS.pdf

id	iium-51185
recordtype	eprints
spelling	iium-511852017-04-05T07:29:35Z http://irep.iium.edu.my/51185/ Cocoa pod husk, a new source of hydrolase enzymes for preparation of cross‑linked enzyme aggregate Yusof, Faridah Khanahmadi, Soofia Amid, Azura Mahmod, Safa Senan T Technology (General) TP155 Chemical engineering Cocoa pod husk (CPH) is a by-product of cocoa production obtained after removing the beans from the fruit. The analysis of CPH has shown that it contains high amounts of protein. This study is aimed to utilize this protein source in hydrolase enzyme production. In this study, seven hydrolase enzymes (amylase, fructosyltransferase, mannanase,glucosidase, glucanase, lipase and protease) were screened from CPH for the first time for feasible industrial production. Among these hydrolases, lipase was chosen for the next steps of experiments as it has a lot of applications in different industries.The extraction of high active lipase from CPH has been done under optimum conditions.The condition that was optimum for the three major factors was achieved using Face centered central composite design (FCCCD) with response surface methodology (RSM) to obtain the highest enzyme activity of crude lipase from CPH. The optimum condition of extraction is used for preparation of cross-linked enzyme aggregate (CLEA). For the production of immobilized biocatalyst, the technique of CLEA is considered as an effective technique for its industrially attractive advantages. Referring to the results of OFAT, CLEA-lipase was prepared in the best condition at the presence of 30 mM ammonium sulphate, 70 mM glutaraldehyde with 0.23 mM Bovine serum albumin as an additive. Immobilization effectively improved the stability of lipase against various organic solvents Springerplus 2016 Article PeerReviewed application/pdf en http://irep.iium.edu.my/51185/1/51185_Cocoa%20pod%20husk.pdf application/pdf en http://irep.iium.edu.my/51185/2/51185_Cocoa%20pod%20husk_WOS.pdf Yusof, Faridah and Khanahmadi, Soofia and Amid, Azura and Mahmod, Safa Senan (2016) Cocoa pod husk, a new source of hydrolase enzymes for preparation of cross‑linked enzyme aggregate. SpringerPlus, 5 (57). pp. 1-18. ISSN 2193-1801 http://springerplus.springeropen.com/articles/10.1186/s40064-015-1621-3 DOI 10.1186/s40064‑015‑1621‑3
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	T Technology (General) TP155 Chemical engineering
spellingShingle	T Technology (General) TP155 Chemical engineering Yusof, Faridah Khanahmadi, Soofia Amid, Azura Mahmod, Safa Senan Cocoa pod husk, a new source of hydrolase enzymes for preparation of cross‑linked enzyme aggregate
description	Cocoa pod husk (CPH) is a by-product of cocoa production obtained after removing the beans from the fruit. The analysis of CPH has shown that it contains high amounts of protein. This study is aimed to utilize this protein source in hydrolase enzyme production. In this study, seven hydrolase enzymes (amylase, fructosyltransferase, mannanase,glucosidase, glucanase, lipase and protease) were screened from CPH for the first time for feasible industrial production. Among these hydrolases, lipase was chosen for the next steps of experiments as it has a lot of applications in different industries.The extraction of high active lipase from CPH has been done under optimum conditions.The condition that was optimum for the three major factors was achieved using Face centered central composite design (FCCCD) with response surface methodology (RSM) to obtain the highest enzyme activity of crude lipase from CPH. The optimum condition of extraction is used for preparation of cross-linked enzyme aggregate (CLEA). For the production of immobilized biocatalyst, the technique of CLEA is considered as an effective technique for its industrially attractive advantages. Referring to the results of OFAT, CLEA-lipase was prepared in the best condition at the presence of 30 mM ammonium sulphate, 70 mM glutaraldehyde with 0.23 mM Bovine serum albumin as an additive. Immobilization effectively improved the stability of lipase against various organic solvents
format	Article
author	Yusof, Faridah Khanahmadi, Soofia Amid, Azura Mahmod, Safa Senan
author_facet	Yusof, Faridah Khanahmadi, Soofia Amid, Azura Mahmod, Safa Senan
author_sort	Yusof, Faridah
title	Cocoa pod husk, a new source of hydrolase enzymes for preparation of cross‑linked enzyme aggregate
title_short	Cocoa pod husk, a new source of hydrolase enzymes for preparation of cross‑linked enzyme aggregate
title_full	Cocoa pod husk, a new source of hydrolase enzymes for preparation of cross‑linked enzyme aggregate
title_fullStr	Cocoa pod husk, a new source of hydrolase enzymes for preparation of cross‑linked enzyme aggregate
title_full_unstemmed	Cocoa pod husk, a new source of hydrolase enzymes for preparation of cross‑linked enzyme aggregate
title_sort	cocoa pod husk, a new source of hydrolase enzymes for preparation of cross‑linked enzyme aggregate
publisher	Springerplus
publishDate	2016
url	http://irep.iium.edu.my/51185/ http://irep.iium.edu.my/51185/ http://irep.iium.edu.my/51185/ http://irep.iium.edu.my/51185/1/51185_Cocoa%20pod%20husk.pdf http://irep.iium.edu.my/51185/2/51185_Cocoa%20pod%20husk_WOS.pdf
first_indexed	2023-09-18T21:12:26Z
last_indexed	2023-09-18T21:12:26Z
_version_	1777411342272036864

Cocoa pod husk, a new source of hydrolase enzymes for preparation of cross‑linked enzyme aggregate

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