PLGA microcapsules with novel dimpled surfaces for pulmonary delivery of DNA

We describe the fabrication of DNA-loaded poly(lactic-co-glycolic acid) (PLGA) microcapsules with novel surface morphologies that will be of use in pulmonary delivery. Our approach was to examine surface morphology and DNA encapsulation efficiency as a function of primary emulsion stability; using...

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Main Authors:	Mohamed, Farahidah, van der Walle, Christopher
Format:	Article
Language:	English
Published:	Elsevier 2006
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/5112/ http://irep.iium.edu.my/5112/ http://irep.iium.edu.my/5112/ http://irep.iium.edu.my/5112/2/1st_published_paper.pdf

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recordtype	eprints
spelling	iium-51122013-01-14T07:31:11Z http://irep.iium.edu.my/5112/ PLGA microcapsules with novel dimpled surfaces for pulmonary delivery of DNA Mohamed, Farahidah van der Walle, Christopher RS Pharmacy and materia medica We describe the fabrication of DNA-loaded poly(lactic-co-glycolic acid) (PLGA) microcapsules with novel surface morphologies that will be of use in pulmonary delivery. Our approach was to examine surface morphology and DNA encapsulation efficiency as a function of primary emulsion stability; using two surfactant series based on hydrophile–lipophile balance and hydrophobe molecular weight. Hydrophilic non-ionic surfactants yielded the most stable water-in-dichloromethane emulsions (HLB values >8). These surfactants normally favor convex (o/w) interfacial curvatures and therefore this atypical behavior suggested a relatively high surfactant solvation in the dichloromethane ‘oil’ phase. This was consistent with the large fall in the glass transition temperature for microspheres prepared with Tween 20, which therefore efficiently penetrated the PLGA matrix and acted as a plasiticizer. Blends of Pluronic triblock copolymers performed poorly as water-in-dichloromethane emulsifiers, and were therefore used to generate hollow microspheres (‘microcapsules’) with low densities (0.24 g/cm3). Although the Pluronic-stabilized emulsions resulted in lower DNA loading (15–28%), microspheres (∼8�m) with novel dimpled surfaces were fabricated. The depth and definition of the dimples was greatest for triblock copolymers with high MW hydrophobe blocks. By cascade impaction, the geometric mean weight diameter of the microcapsules was 3.43�m, suggesting that they will be of interest as biodegradable pulmonary delivery vehicles. Elsevier 2006-01-18 Article PeerReviewed application/pdf en http://irep.iium.edu.my/5112/2/1st_published_paper.pdf Mohamed, Farahidah and van der Walle, Christopher (2006) PLGA microcapsules with novel dimpled surfaces for pulmonary delivery of DNA. International Journal of Pharmaceutics, 311. pp. 97-107. ISSN 0378-5173 http://www.journals.elsevier.com/international-journal-of-pharmaceutics/ doi:10.1016/j.ijpharm.2005.12.016
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Mohamed, Farahidah van der Walle, Christopher PLGA microcapsules with novel dimpled surfaces for pulmonary delivery of DNA
description	We describe the fabrication of DNA-loaded poly(lactic-co-glycolic acid) (PLGA) microcapsules with novel surface morphologies that will be of use in pulmonary delivery. Our approach was to examine surface morphology and DNA encapsulation efficiency as a function of primary emulsion stability; using two surfactant series based on hydrophile–lipophile balance and hydrophobe molecular weight. Hydrophilic non-ionic surfactants yielded the most stable water-in-dichloromethane emulsions (HLB values >8). These surfactants normally favor convex (o/w) interfacial curvatures and therefore this atypical behavior suggested a relatively high surfactant solvation in the dichloromethane ‘oil’ phase. This was consistent with the large fall in the glass transition temperature for microspheres prepared with Tween 20, which therefore efficiently penetrated the PLGA matrix and acted as a plasiticizer. Blends of Pluronic triblock copolymers performed poorly as water-in-dichloromethane emulsifiers, and were therefore used to generate hollow microspheres (‘microcapsules’) with low densities (0.24 g/cm3). Although the Pluronic-stabilized emulsions resulted in lower DNA loading (15–28%), microspheres (∼8�m) with novel dimpled surfaces were fabricated. The depth and definition of the dimples was greatest for triblock copolymers with high MW hydrophobe blocks. By cascade impaction, the geometric mean weight diameter of the microcapsules was 3.43�m, suggesting that they will be of interest as biodegradable pulmonary delivery vehicles.
format	Article
author	Mohamed, Farahidah van der Walle, Christopher
author_facet	Mohamed, Farahidah van der Walle, Christopher
author_sort	Mohamed, Farahidah
title	PLGA microcapsules with novel dimpled surfaces for pulmonary delivery of DNA
title_short	PLGA microcapsules with novel dimpled surfaces for pulmonary delivery of DNA
title_full	PLGA microcapsules with novel dimpled surfaces for pulmonary delivery of DNA
title_fullStr	PLGA microcapsules with novel dimpled surfaces for pulmonary delivery of DNA
title_full_unstemmed	PLGA microcapsules with novel dimpled surfaces for pulmonary delivery of DNA
title_sort	plga microcapsules with novel dimpled surfaces for pulmonary delivery of dna
publisher	Elsevier
publishDate	2006
url	http://irep.iium.edu.my/5112/ http://irep.iium.edu.my/5112/ http://irep.iium.edu.my/5112/ http://irep.iium.edu.my/5112/2/1st_published_paper.pdf
first_indexed	2023-09-18T20:13:36Z
last_indexed	2023-09-18T20:13:36Z
_version_	1777407640111939584

PLGA microcapsules with novel dimpled surfaces for pulmonary delivery of DNA

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