Adipose tissue in halal meat can be a source of adiponectin protein
The objective of this study was to isolate and quantify total protein and adiponectin from halal meat sources of chicken, beef and lamb adipose tissues. Abdominal adipose tissues were removed from chicken, beef and lamb rinsed with ice-cold phosphate-buffered saline (PBS) solution. Delipidation of a...
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2016
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iium-510952019-09-03T02:19:56Z http://irep.iium.edu.my/51095/ Adipose tissue in halal meat can be a source of adiponectin protein Azahari , Nuraniza Khattak, Muhammad Muzaffar Ali Khan Mohd Shukri, Nor Azwani QP Physiology TX341 Nutrition. Foods and food supply The objective of this study was to isolate and quantify total protein and adiponectin from halal meat sources of chicken, beef and lamb adipose tissues. Abdominal adipose tissues were removed from chicken, beef and lamb rinsed with ice-cold phosphate-buffered saline (PBS) solution. Delipidation of aforementioned tissues were prepared by chloroform/methanol extractions modified from Bligh and Dyer method. The protein concentration was determined by using Protein Assay Bicinchoninate Kit. After that, quantification of the adiponectin protein in chicken, beef and lamb tissues were assessed by using ELISA assay kit according to manufacturer’s instructions. The experiment was conducted in triplicates and the results were presented as means ± SD. Results indicate that the extraction of 10 gram subcutaneous adipose tissues from chicken, beef and lamb yielded about 0.1 gram of protein amount which was only 1 % from total tissue mass. The protein concentration in adipose tissue of chicken, beef and lamb were 1.25 ± 0.05, 1.75 ± 0.05 & 2.53 ± 0.07 mg/ml respectively. Then, Adiponectin levels in chicken, beef and lamb were 158 ± 4.5 ng/ml, 24 ± 0.5 µg/ml and 37 ± 0.98 ng/ml respectively. Adiponectin is one of the promising candidate for future pharmacological treatment strategies for insulin resistance and metabolic diseases. 2016-07 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/51095/7/51095.pdf Azahari , Nuraniza and Khattak, Muhammad Muzaffar Ali Khan and Mohd Shukri, Nor Azwani (2016) Adipose tissue in halal meat can be a source of adiponectin protein. In: 5th Annual Global Healthcare Conference (GHC 2016), 18th-19th July 2016, Singapore. (Unpublished) |
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Digital Repository |
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Local University |
| institution |
International Islamic University Malaysia |
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IIUM Repository |
| collection |
Online Access |
| language |
English |
| topic |
QP Physiology TX341 Nutrition. Foods and food supply |
| spellingShingle |
QP Physiology TX341 Nutrition. Foods and food supply Azahari , Nuraniza Khattak, Muhammad Muzaffar Ali Khan Mohd Shukri, Nor Azwani Adipose tissue in halal meat can be a source of adiponectin protein |
| description |
The objective of this study was to isolate and quantify total protein and adiponectin from halal meat sources of chicken, beef and lamb adipose tissues. Abdominal adipose tissues were removed from chicken, beef and lamb rinsed with ice-cold phosphate-buffered saline (PBS) solution. Delipidation of aforementioned tissues were prepared by chloroform/methanol extractions modified from Bligh and Dyer method. The protein concentration was determined by using Protein Assay Bicinchoninate Kit. After that, quantification of the adiponectin protein in chicken, beef and lamb tissues were assessed by using ELISA assay kit according to manufacturer’s instructions. The experiment was conducted in triplicates and the results were presented as means ± SD. Results indicate that the extraction of 10 gram subcutaneous adipose tissues from chicken, beef and lamb yielded about 0.1 gram of protein amount which was only 1 % from total tissue mass. The protein concentration in adipose tissue of chicken, beef and lamb were 1.25 ± 0.05, 1.75 ± 0.05 & 2.53 ± 0.07 mg/ml respectively. Then, Adiponectin levels in chicken, beef and lamb were 158 ± 4.5 ng/ml, 24 ± 0.5 µg/ml and 37 ± 0.98 ng/ml respectively. Adiponectin is one of the promising candidate for future pharmacological treatment strategies for insulin resistance and metabolic diseases. |
| format |
Conference or Workshop Item |
| author |
Azahari , Nuraniza Khattak, Muhammad Muzaffar Ali Khan Mohd Shukri, Nor Azwani |
| author_facet |
Azahari , Nuraniza Khattak, Muhammad Muzaffar Ali Khan Mohd Shukri, Nor Azwani |
| author_sort |
Azahari , Nuraniza |
| title |
Adipose tissue in halal meat can be a source of
adiponectin protein |
| title_short |
Adipose tissue in halal meat can be a source of
adiponectin protein |
| title_full |
Adipose tissue in halal meat can be a source of
adiponectin protein |
| title_fullStr |
Adipose tissue in halal meat can be a source of
adiponectin protein |
| title_full_unstemmed |
Adipose tissue in halal meat can be a source of
adiponectin protein |
| title_sort |
adipose tissue in halal meat can be a source of
adiponectin protein |
| publishDate |
2016 |
| url |
http://irep.iium.edu.my/51095/ http://irep.iium.edu.my/51095/7/51095.pdf |
| first_indexed |
2023-09-18T21:12:18Z |
| last_indexed |
2023-09-18T21:12:18Z |
| _version_ |
1777411333217583104 |