Screening of various parameters of enterobacter aerogenes batch culture for biohydrogen production

Hydrogen (H2) gas has been widely known as a potential renewable energy. Throughout the advancements of technologies, a method of producing H2 gas which is known as biohydrogen was developed. Biohydrogen has a potential to solve the current greenhouse effects since it is an eco-friendly and harmle...

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Main Authors:	Mohamad Puad, Noor Illi, Mamat, Nur Amira, Azmi, Azlin Suhaida
Format:	Conference or Workshop Item
Language:	English
Published:	International Association of Computer Science & Information Technology (IACSIT ) Press 2016
Subjects:	T Technology (General)
Online Access:	http://irep.iium.edu.my/51018/ http://irep.iium.edu.my/51018/ http://irep.iium.edu.my/51018/ http://irep.iium.edu.my/51018/15/51018-new.pdf

id	iium-51018
recordtype	eprints
spelling	iium-510182017-01-16T00:23:17Z http://irep.iium.edu.my/51018/ Screening of various parameters of enterobacter aerogenes batch culture for biohydrogen production Mohamad Puad, Noor Illi Mamat, Nur Amira Azmi, Azlin Suhaida T Technology (General) Hydrogen (H2) gas has been widely known as a potential renewable energy. Throughout the advancements of technologies, a method of producing H2 gas which is known as biohydrogen was developed. Biohydrogen has a potential to solve the current greenhouse effects since it is an eco-friendly and harmless gas that is operated at ambient temperature and atmospheric pressure using renewable substrates as a source. In this study, Enterobacter aerogenes (E. aerogenes) was used as the biohydrogen producing microorganism. The parameters that influence the biohydrogen production such as glucose, glycerol, initial pH, inoculum size, tryptone, yeast extract, agitation rate and temperature were screened using Plackett-Burman design tool (Design Expert 6.08 software). The results were analyzed using Minitab 17 software and fitted into the secondary-order polynomial regression equation for further analysis. Based on the results, for the bioreactor fermentation, glucose, glycerol, tryptone, inoculum size and agitation rate have negative effects to the biohydrogen production whereas initial pH, yeast extract and temperature gave positive effects. For the shake flask experiments, some of the low level of the parameters gave greater influence on hydrogen yield (glucose, tryptone, agitation rate and temperature) while some of the high level of the parameters gave greater influenced on the hydrogen yield (glycerol, initial pH, inoculum size and yeast extract). The highest hydrogen yield achieved in the bioreactor was 10 ppm while in the shake flask experiments, the highest yield recorded was 24 ppm. International Association of Computer Science & Information Technology (IACSIT ) Press 2016 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/51018/15/51018-new.pdf Mohamad Puad, Noor Illi and Mamat, Nur Amira and Azmi, Azlin Suhaida (2016) Screening of various parameters of enterobacter aerogenes batch culture for biohydrogen production. In: 2016 5th International Conference on Environment, Energy and Biotechnology (ICEEB 2016), 23rd-25th May 2016, Jeju Island, Republic of Korea. http://www.ipcbee.com/list-29-1.html 10.7763/IPCBEE.
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	T Technology (General)
spellingShingle	T Technology (General) Mohamad Puad, Noor Illi Mamat, Nur Amira Azmi, Azlin Suhaida Screening of various parameters of enterobacter aerogenes batch culture for biohydrogen production
description	Hydrogen (H2) gas has been widely known as a potential renewable energy. Throughout the advancements of technologies, a method of producing H2 gas which is known as biohydrogen was developed. Biohydrogen has a potential to solve the current greenhouse effects since it is an eco-friendly and harmless gas that is operated at ambient temperature and atmospheric pressure using renewable substrates as a source. In this study, Enterobacter aerogenes (E. aerogenes) was used as the biohydrogen producing microorganism. The parameters that influence the biohydrogen production such as glucose, glycerol, initial pH, inoculum size, tryptone, yeast extract, agitation rate and temperature were screened using Plackett-Burman design tool (Design Expert 6.08 software). The results were analyzed using Minitab 17 software and fitted into the secondary-order polynomial regression equation for further analysis. Based on the results, for the bioreactor fermentation, glucose, glycerol, tryptone, inoculum size and agitation rate have negative effects to the biohydrogen production whereas initial pH, yeast extract and temperature gave positive effects. For the shake flask experiments, some of the low level of the parameters gave greater influence on hydrogen yield (glucose, tryptone, agitation rate and temperature) while some of the high level of the parameters gave greater influenced on the hydrogen yield (glycerol, initial pH, inoculum size and yeast extract). The highest hydrogen yield achieved in the bioreactor was 10 ppm while in the shake flask experiments, the highest yield recorded was 24 ppm.
format	Conference or Workshop Item
author	Mohamad Puad, Noor Illi Mamat, Nur Amira Azmi, Azlin Suhaida
author_facet	Mohamad Puad, Noor Illi Mamat, Nur Amira Azmi, Azlin Suhaida
author_sort	Mohamad Puad, Noor Illi
title	Screening of various parameters of enterobacter aerogenes batch culture for biohydrogen production
title_short	Screening of various parameters of enterobacter aerogenes batch culture for biohydrogen production
title_full	Screening of various parameters of enterobacter aerogenes batch culture for biohydrogen production
title_fullStr	Screening of various parameters of enterobacter aerogenes batch culture for biohydrogen production
title_full_unstemmed	Screening of various parameters of enterobacter aerogenes batch culture for biohydrogen production
title_sort	screening of various parameters of enterobacter aerogenes batch culture for biohydrogen production
publisher	International Association of Computer Science & Information Technology (IACSIT ) Press
publishDate	2016
url	http://irep.iium.edu.my/51018/ http://irep.iium.edu.my/51018/ http://irep.iium.edu.my/51018/ http://irep.iium.edu.my/51018/15/51018-new.pdf
first_indexed	2023-09-18T21:12:11Z
last_indexed	2023-09-18T21:12:11Z
_version_	1777411326458462208

Screening of various parameters of enterobacter aerogenes batch culture for biohydrogen production

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