Antimicrobial Susceptibility of Klebsiella pneumoniae and Escherichia coli with Extended-Spectrum β-lactamase associated Genes in Hospital Tengku Ampuan Afzan,Kuantan, Pahang

Background: To assess antimicrobial susceptibility of extended-spectrum β-lactamase- (ESBL-) producing Klebsiella pneumoniae and Escherichia coli isolates from Hospital Tengku Ampuan Afzan (HTAA), as well as to identify ESBL genes. Methods: Non-duplicate K. pneumoniae and E. coli isolates w...

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Main Authors:	Yahya Mohsen, Saleh Mahdi, Hamzah, Hairul Aini, Mustafa Mahmoud, Mohammed Imad Al-Deen, Baharudin, Roesnita
Format:	Article
Language:	English English
Published:	Universiti Sains Malaysia 2016
Subjects:	QR Microbiology
Online Access:	http://irep.iium.edu.my/50909/ http://irep.iium.edu.my/50909/ http://irep.iium.edu.my/50909/1/50909_-_Antimicrobial_Susceptibility_of_Klebsiella_pneumoniae_and_Escherichia_coli_with_Extended-Spectrum_%CE%B2-lactamase_associated_Genes_in_Hospital_Tengku_Ampuan_Afzan%2CKuantan%2C_Pahang.pdf http://irep.iium.edu.my/50909/4/50909_Antimicrobial%20Susceptibility%20of%20Klebsiella%20%281%29.pdf

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recordtype	eprints
spelling	iium-509092017-08-03T07:48:47Z http://irep.iium.edu.my/50909/ Antimicrobial Susceptibility of Klebsiella pneumoniae and Escherichia coli with Extended-Spectrum β-lactamase associated Genes in Hospital Tengku Ampuan Afzan,Kuantan, Pahang Yahya Mohsen, Saleh Mahdi Hamzah, Hairul Aini Mustafa Mahmoud, Mohammed Imad Al-Deen Baharudin, Roesnita QR Microbiology Background: To assess antimicrobial susceptibility of extended-spectrum β-lactamase- (ESBL-) producing Klebsiella pneumoniae and Escherichia coli isolates from Hospital Tengku Ampuan Afzan (HTAA), as well as to identify ESBL genes. Methods: Non-duplicate K. pneumoniae and E. coli isolates were recovered from various clinical samples. Isolates were screened for antimicrobial resistance by disc diffusion method. Isolates resistant to oxyimino-cephalosporins were subjected to phenotypic ESBL production. Detection of resistance genes was then performed using primers specific for ESBL genes(bla CTX-M,bla SHV and bla TEM). Results: Piperacillin/tazobactam and carbapenems remained the active β-lactam antibioti against K. pneumoniae and E. coli. ESBLs were detected among 35.5%(39/110)of K. pneumoniae and 18.8%(28/149)of E. coli isolates. CTX-M β-lactamase was detected in 90% of all ESBL-positive isolates, whereas bla SHV and bla TEM genes were found among 56% and 52% of them, respectively. Twenty-eight percent(28%)of the total ESBL-positive isolates harboured the three ESBL genes, while 50% carried two of the tested ESBL genes. Conclusion ESBLs encoded by at least one ESBL genes are frequently isolated among K. pneumoniae and E. coli in HTAA. The significant proportion rate of the resistant determinants is alarming, thus monitoring their transmission and dissemination is essential to control it at an early phase. Universiti Sains Malaysia 2016-03 Article PeerReviewed application/pdf en http://irep.iium.edu.my/50909/1/50909_-_Antimicrobial_Susceptibility_of_Klebsiella_pneumoniae_and_Escherichia_coli_with_Extended-Spectrum_%CE%B2-lactamase_associated_Genes_in_Hospital_Tengku_Ampuan_Afzan%2CKuantan%2C_Pahang.pdf application/pdf en http://irep.iium.edu.my/50909/4/50909_Antimicrobial%20Susceptibility%20of%20Klebsiella%20%281%29.pdf Yahya Mohsen, Saleh Mahdi and Hamzah, Hairul Aini and Mustafa Mahmoud, Mohammed Imad Al-Deen and Baharudin, Roesnita (2016) Antimicrobial Susceptibility of Klebsiella pneumoniae and Escherichia coli with Extended-Spectrum β-lactamase associated Genes in Hospital Tengku Ampuan Afzan,Kuantan, Pahang. Malaysian Journal of Medical Sciences, 23 (2). pp. 14-20. ISSN 1394-195X E-ISSN 2180-4303 http://www.mjms.usm.my/default.asp
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	QR Microbiology
spellingShingle	QR Microbiology Yahya Mohsen, Saleh Mahdi Hamzah, Hairul Aini Mustafa Mahmoud, Mohammed Imad Al-Deen Baharudin, Roesnita Antimicrobial Susceptibility of Klebsiella pneumoniae and Escherichia coli with Extended-Spectrum β-lactamase associated Genes in Hospital Tengku Ampuan Afzan,Kuantan, Pahang
description	Background: To assess antimicrobial susceptibility of extended-spectrum β-lactamase- (ESBL-) producing Klebsiella pneumoniae and Escherichia coli isolates from Hospital Tengku Ampuan Afzan (HTAA), as well as to identify ESBL genes. Methods: Non-duplicate K. pneumoniae and E. coli isolates were recovered from various clinical samples. Isolates were screened for antimicrobial resistance by disc diffusion method. Isolates resistant to oxyimino-cephalosporins were subjected to phenotypic ESBL production. Detection of resistance genes was then performed using primers specific for ESBL genes(bla CTX-M,bla SHV and bla TEM). Results: Piperacillin/tazobactam and carbapenems remained the active β-lactam antibioti against K. pneumoniae and E. coli. ESBLs were detected among 35.5%(39/110)of K. pneumoniae and 18.8%(28/149)of E. coli isolates. CTX-M β-lactamase was detected in 90% of all ESBL-positive isolates, whereas bla SHV and bla TEM genes were found among 56% and 52% of them, respectively. Twenty-eight percent(28%)of the total ESBL-positive isolates harboured the three ESBL genes, while 50% carried two of the tested ESBL genes. Conclusion ESBLs encoded by at least one ESBL genes are frequently isolated among K. pneumoniae and E. coli in HTAA. The significant proportion rate of the resistant determinants is alarming, thus monitoring their transmission and dissemination is essential to control it at an early phase.
format	Article
author	Yahya Mohsen, Saleh Mahdi Hamzah, Hairul Aini Mustafa Mahmoud, Mohammed Imad Al-Deen Baharudin, Roesnita
author_facet	Yahya Mohsen, Saleh Mahdi Hamzah, Hairul Aini Mustafa Mahmoud, Mohammed Imad Al-Deen Baharudin, Roesnita
author_sort	Yahya Mohsen, Saleh Mahdi
title	Antimicrobial Susceptibility of Klebsiella pneumoniae and Escherichia coli with Extended-Spectrum β-lactamase associated Genes in Hospital Tengku Ampuan Afzan,Kuantan, Pahang
title_short	Antimicrobial Susceptibility of Klebsiella pneumoniae and Escherichia coli with Extended-Spectrum β-lactamase associated Genes in Hospital Tengku Ampuan Afzan,Kuantan, Pahang
title_full	Antimicrobial Susceptibility of Klebsiella pneumoniae and Escherichia coli with Extended-Spectrum β-lactamase associated Genes in Hospital Tengku Ampuan Afzan,Kuantan, Pahang
title_fullStr	Antimicrobial Susceptibility of Klebsiella pneumoniae and Escherichia coli with Extended-Spectrum β-lactamase associated Genes in Hospital Tengku Ampuan Afzan,Kuantan, Pahang
title_full_unstemmed	Antimicrobial Susceptibility of Klebsiella pneumoniae and Escherichia coli with Extended-Spectrum β-lactamase associated Genes in Hospital Tengku Ampuan Afzan,Kuantan, Pahang
title_sort	antimicrobial susceptibility of klebsiella pneumoniae and escherichia coli with extended-spectrum β-lactamase associated genes in hospital tengku ampuan afzan,kuantan, pahang
publisher	Universiti Sains Malaysia
publishDate	2016
url	http://irep.iium.edu.my/50909/ http://irep.iium.edu.my/50909/ http://irep.iium.edu.my/50909/1/50909_-_Antimicrobial_Susceptibility_of_Klebsiella_pneumoniae_and_Escherichia_coli_with_Extended-Spectrum_%CE%B2-lactamase_associated_Genes_in_Hospital_Tengku_Ampuan_Afzan%2CKuantan%2C_Pahang.pdf http://irep.iium.edu.my/50909/4/50909_Antimicrobial%20Susceptibility%20of%20Klebsiella%20%281%29.pdf
first_indexed	2023-09-18T21:12:02Z
last_indexed	2023-09-18T21:12:02Z
_version_	1777411317129281536

Antimicrobial Susceptibility of Klebsiella pneumoniae and Escherichia coli with Extended-Spectrum β-lactamase associated Genes in Hospital Tengku Ampuan Afzan,Kuantan, Pahang

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