In vitro anticancer activity of Au, Ag nanoparticles synthesized using Commelina nudiflora L. aqueous extract against HCT-116 colon cancer cells

Recently, metal nanoparticles have been getting great medical and social interests due to their potential physico-chemical properties such as higher affinity, low molecular weight, and larger surface area. The biosynthesized gold and silver nanoparticles are spherical, triangular in shape with an av...

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Main Authors:	Kuppusamy, Palaniselvam, Ichwan, Solachuddin J. A., Al-Zikri, Putri Nur Hidayah, Suriyah, Wastuti Hidayati, Soundharrajan, Ilavenil, Govindan, Natanamurugaraj, Maniam, Gaanty Pragas, Yusoff, Mashitah
Format:	Article
Language:	English English
Published:	Springer 2016
Subjects:	RB Pathology RM Therapeutics. Pharmacology RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/50688/ http://irep.iium.edu.my/50688/ http://irep.iium.edu.my/50688/ http://irep.iium.edu.my/50688/1/Biological_Trace_Elements.pdf http://irep.iium.edu.my/50688/4/50688-In_vitro_anticancer_activity_of_Au%2C_Ag_nanoparticles_synthesized_SCOPUS-In-press.pdf

id	iium-50688
recordtype	eprints
spelling	iium-506882016-12-30T07:10:01Z http://irep.iium.edu.my/50688/ In vitro anticancer activity of Au, Ag nanoparticles synthesized using Commelina nudiflora L. aqueous extract against HCT-116 colon cancer cells Kuppusamy, Palaniselvam Ichwan, Solachuddin J. A. Al-Zikri, Putri Nur Hidayah Suriyah, Wastuti Hidayati Soundharrajan, Ilavenil Govindan, Natanamurugaraj Maniam, Gaanty Pragas Yusoff, Mashitah RB Pathology RM Therapeutics. Pharmacology RS Pharmacy and materia medica Recently, metal nanoparticles have been getting great medical and social interests due to their potential physico-chemical properties such as higher affinity, low molecular weight, and larger surface area. The biosynthesized gold and silver nanoparticles are spherical, triangular in shape with an average size of 24–150 nm as reported in our earlier studies. The biological properties of synthesized gold and silver nanoparticles are demonstrated in this paper. The different in vitro assays such as MTT, flow cytometry, and reverse transcription polymerase chain reaction (RT-qPCR) techniques were used to evaluate the in vitro anticancer properties of synthesized metal nanoparticles. The biosynthesized gold and silver nanoparticles have shown reduced cell viability and increased cytotoxicity in HCT-116 colon cancer cells with IC50 concentration of 200 and 100 μg/ml, respectively. The flow cytometry experiments revealed that the IC50 concentrations of gold and silver nanoparticle-treated cells that have significant changes were observed in the sub-G1 cell cycle phase compared with the positive control. Additionally, the relative messenger RNA (mRNA) gene expressions of HCT-116 cells were studied by RT-qPCR techniques. The pro-apoptotic genes such as PUMA (++), Caspase-3 (+), Caspase-8 (++), and Caspase-9 (++) were upregulated in the treated HCT-116 cells compared with cisplatin. Overall, these findings have proved that the synthesized gold and silver nanoparticles could be potent anti-colon cancer drugs. Springer 2016-03-09 Article PeerReviewed application/pdf en http://irep.iium.edu.my/50688/1/Biological_Trace_Elements.pdf application/pdf en http://irep.iium.edu.my/50688/4/50688-In_vitro_anticancer_activity_of_Au%2C_Ag_nanoparticles_synthesized_SCOPUS-In-press.pdf Kuppusamy, Palaniselvam and Ichwan, Solachuddin J. A. and Al-Zikri, Putri Nur Hidayah and Suriyah, Wastuti Hidayati and Soundharrajan, Ilavenil and Govindan, Natanamurugaraj and Maniam, Gaanty Pragas and Yusoff, Mashitah (2016) In vitro anticancer activity of Au, Ag nanoparticles synthesized using Commelina nudiflora L. aqueous extract against HCT-116 colon cancer cells. Biological Trace Element Research, 173 (2). pp. 1-9. ISSN 01634984 E-ISSN 1559-0720 (In Press) http://link.springer.com/article/10.1007%2Fs12011-016-0666-7 10.1007/s12011-016-0666-7
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RB Pathology RM Therapeutics. Pharmacology RS Pharmacy and materia medica
spellingShingle	RB Pathology RM Therapeutics. Pharmacology RS Pharmacy and materia medica Kuppusamy, Palaniselvam Ichwan, Solachuddin J. A. Al-Zikri, Putri Nur Hidayah Suriyah, Wastuti Hidayati Soundharrajan, Ilavenil Govindan, Natanamurugaraj Maniam, Gaanty Pragas Yusoff, Mashitah In vitro anticancer activity of Au, Ag nanoparticles synthesized using Commelina nudiflora L. aqueous extract against HCT-116 colon cancer cells
description	Recently, metal nanoparticles have been getting great medical and social interests due to their potential physico-chemical properties such as higher affinity, low molecular weight, and larger surface area. The biosynthesized gold and silver nanoparticles are spherical, triangular in shape with an average size of 24–150 nm as reported in our earlier studies. The biological properties of synthesized gold and silver nanoparticles are demonstrated in this paper. The different in vitro assays such as MTT, flow cytometry, and reverse transcription polymerase chain reaction (RT-qPCR) techniques were used to evaluate the in vitro anticancer properties of synthesized metal nanoparticles. The biosynthesized gold and silver nanoparticles have shown reduced cell viability and increased cytotoxicity in HCT-116 colon cancer cells with IC50 concentration of 200 and 100 μg/ml, respectively. The flow cytometry experiments revealed that the IC50 concentrations of gold and silver nanoparticle-treated cells that have significant changes were observed in the sub-G1 cell cycle phase compared with the positive control. Additionally, the relative messenger RNA (mRNA) gene expressions of HCT-116 cells were studied by RT-qPCR techniques. The pro-apoptotic genes such as PUMA (++), Caspase-3 (+), Caspase-8 (++), and Caspase-9 (++) were upregulated in the treated HCT-116 cells compared with cisplatin. Overall, these findings have proved that the synthesized gold and silver nanoparticles could be potent anti-colon cancer drugs.
format	Article
author	Kuppusamy, Palaniselvam Ichwan, Solachuddin J. A. Al-Zikri, Putri Nur Hidayah Suriyah, Wastuti Hidayati Soundharrajan, Ilavenil Govindan, Natanamurugaraj Maniam, Gaanty Pragas Yusoff, Mashitah
author_facet	Kuppusamy, Palaniselvam Ichwan, Solachuddin J. A. Al-Zikri, Putri Nur Hidayah Suriyah, Wastuti Hidayati Soundharrajan, Ilavenil Govindan, Natanamurugaraj Maniam, Gaanty Pragas Yusoff, Mashitah
author_sort	Kuppusamy, Palaniselvam
title	In vitro anticancer activity of Au, Ag nanoparticles synthesized using Commelina nudiflora L. aqueous extract against HCT-116 colon cancer cells
title_short	In vitro anticancer activity of Au, Ag nanoparticles synthesized using Commelina nudiflora L. aqueous extract against HCT-116 colon cancer cells
title_full	In vitro anticancer activity of Au, Ag nanoparticles synthesized using Commelina nudiflora L. aqueous extract against HCT-116 colon cancer cells
title_fullStr	In vitro anticancer activity of Au, Ag nanoparticles synthesized using Commelina nudiflora L. aqueous extract against HCT-116 colon cancer cells
title_full_unstemmed	In vitro anticancer activity of Au, Ag nanoparticles synthesized using Commelina nudiflora L. aqueous extract against HCT-116 colon cancer cells
title_sort	in vitro anticancer activity of au, ag nanoparticles synthesized using commelina nudiflora l. aqueous extract against hct-116 colon cancer cells
publisher	Springer
publishDate	2016
url	http://irep.iium.edu.my/50688/ http://irep.iium.edu.my/50688/ http://irep.iium.edu.my/50688/ http://irep.iium.edu.my/50688/1/Biological_Trace_Elements.pdf http://irep.iium.edu.my/50688/4/50688-In_vitro_anticancer_activity_of_Au%2C_Ag_nanoparticles_synthesized_SCOPUS-In-press.pdf
first_indexed	2023-09-18T21:11:40Z
last_indexed	2023-09-18T21:11:40Z
_version_	1777411293878157312

In vitro anticancer activity of Au, Ag nanoparticles synthesized using Commelina nudiflora L. aqueous extract against HCT-116 colon cancer cells

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