Optimization of lignin peroxidase production and stability by Phanerochaete chrysosporium using sewage-treatment-plant sludge as substrate in a stirred-tank bioreactor
A laboratory-scale study was carried out to produce lignin peroxidase (ligninase) by white rot fungus (Phanerochaete chrysosporium) using sewage-treatmentplant (STP) sludge as the major substrate. The optimization was done using full-factorial design (FFD) with agitation and aeration as the two para...
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iium-50652017-12-07T04:36:52Z http://irep.iium.edu.my/5065/ Optimization of lignin peroxidase production and stability by Phanerochaete chrysosporium using sewage-treatment-plant sludge as substrate in a stirred-tank bioreactor Alam, Md. Zahangir Mansor, Mariatul F. Khan Chowdhury, Ahmed Jalal QP Physiology TP Chemical technology TP248.13 Biotechnology A laboratory-scale study was carried out to produce lignin peroxidase (ligninase) by white rot fungus (Phanerochaete chrysosporium) using sewage-treatmentplant (STP) sludge as the major substrate. The optimization was done using full-factorial design (FFD) with agitation and aeration as the two parameters. Nine experiments indicated by the FFD were fermented in a stirred-tank bioreactor for 3 days. A second-order quadratic model was developed using the regression analysis of the experimental results with the linear, quadratic, and interaction effects of the parameters. Analysis of variance (ANOVA) showed a high coefficient of determination (R2) value of 0.972, thus indicating a satisfactory fit of the quadratic model with the experimental data. Using statistical analysis, the optimum aeration and agitation rates were determined to be 2.0 vvm and 200 rpm, respectively, with a maximum activity of 225 U l-1 in the first 3 days of fermentation. The validation experiment showed the maximum activity of lignin peroxidase was 744 U l-1 after 5 days of fermentation. The results for the tests of the stability of lignin peroxidase showed that the activity was more than 80% of the maximum for the first 12 h of incubation at an optimum pH of 5 and temperature of 55�C. Springer 2009 Article PeerReviewed application/pdf en http://irep.iium.edu.my/5065/1/Paper-LiP-JIMB.pdf Alam, Md. Zahangir and Mansor, Mariatul F. and Khan Chowdhury, Ahmed Jalal (2009) Optimization of lignin peroxidase production and stability by Phanerochaete chrysosporium using sewage-treatment-plant sludge as substrate in a stirred-tank bioreactor. Journal of Industrial Microbiology & Biotechnology, 36 (5). pp. 757-764. ISSN 1367-5435 http://dx.doi.org/10.1007/s10295-009-0548-5 doi:10.1007/s10295-009-0548-5 |
| repository_type |
Digital Repository |
| institution_category |
Local University |
| institution |
International Islamic University Malaysia |
| building |
IIUM Repository |
| collection |
Online Access |
| language |
English |
| topic |
QP Physiology TP Chemical technology TP248.13 Biotechnology |
| spellingShingle |
QP Physiology TP Chemical technology TP248.13 Biotechnology Alam, Md. Zahangir Mansor, Mariatul F. Khan Chowdhury, Ahmed Jalal Optimization of lignin peroxidase production and stability by Phanerochaete chrysosporium using sewage-treatment-plant sludge as substrate in a stirred-tank bioreactor |
| description |
A laboratory-scale study was carried out to produce lignin peroxidase (ligninase) by white rot fungus (Phanerochaete chrysosporium) using sewage-treatmentplant (STP) sludge as the major substrate. The optimization was done using full-factorial design (FFD) with agitation and aeration as the two parameters. Nine experiments indicated by the FFD were fermented in a stirred-tank bioreactor for 3 days. A second-order quadratic model was developed using the regression analysis of the experimental results with the linear, quadratic, and interaction effects of
the parameters. Analysis of variance (ANOVA) showed a
high coefficient of determination (R2) value of 0.972, thus
indicating a satisfactory fit of the quadratic model with the experimental data. Using statistical analysis, the optimum aeration and agitation rates were determined to be 2.0 vvm and 200 rpm, respectively, with a maximum activity of 225 U l-1 in the first 3 days of fermentation. The validation experiment showed the maximum activity of lignin
peroxidase was 744 U l-1 after 5 days of fermentation. The results for the tests of the stability of lignin peroxidase
showed that the activity was more than 80% of the maximum
for the first 12 h of incubation at an optimum pH of 5
and temperature of 55�C. |
| format |
Article |
| author |
Alam, Md. Zahangir Mansor, Mariatul F. Khan Chowdhury, Ahmed Jalal |
| author_facet |
Alam, Md. Zahangir Mansor, Mariatul F. Khan Chowdhury, Ahmed Jalal |
| author_sort |
Alam, Md. Zahangir |
| title |
Optimization of lignin peroxidase production and stability by Phanerochaete chrysosporium using sewage-treatment-plant sludge as substrate in a stirred-tank bioreactor |
| title_short |
Optimization of lignin peroxidase production and stability by Phanerochaete chrysosporium using sewage-treatment-plant sludge as substrate in a stirred-tank bioreactor |
| title_full |
Optimization of lignin peroxidase production and stability by Phanerochaete chrysosporium using sewage-treatment-plant sludge as substrate in a stirred-tank bioreactor |
| title_fullStr |
Optimization of lignin peroxidase production and stability by Phanerochaete chrysosporium using sewage-treatment-plant sludge as substrate in a stirred-tank bioreactor |
| title_full_unstemmed |
Optimization of lignin peroxidase production and stability by Phanerochaete chrysosporium using sewage-treatment-plant sludge as substrate in a stirred-tank bioreactor |
| title_sort |
optimization of lignin peroxidase production and stability by phanerochaete chrysosporium using sewage-treatment-plant sludge as substrate in a stirred-tank bioreactor |
| publisher |
Springer |
| publishDate |
2009 |
| url |
http://irep.iium.edu.my/5065/ http://irep.iium.edu.my/5065/ http://irep.iium.edu.my/5065/ http://irep.iium.edu.my/5065/1/Paper-LiP-JIMB.pdf |
| first_indexed |
2023-09-18T20:13:33Z |
| last_indexed |
2023-09-18T20:13:33Z |
| _version_ |
1777407637335310336 |